BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0075.0051.p
         (396 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312272|ref|YP_003776364.1| dihydrolipoamide succinyltransf...    98   1e-21
gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransfer...    77   3e-15

>gi|300312272|ref|YP_003776364.1| dihydrolipoamide
           succinyltransferase component of 2-oxoglutarate
           dehydrogenase complex protein [Herbaspirillum
           seropedicae SmR1]
          Length = 413

 Score = 98.2 bits (243), Expect = 1e-21,   Method: Compositional matrix adjust.
 Identities = 97/368 (26%), Positives = 155/368 (42%), Gaps = 30/368 (8%)

Query: 3   ILIRMPEIAANATSAVIKEWSRKEGDSIRIGETLAEIETDKALIEFDADQAGILGKILAP 62
           I +++P+++ +   A + +W +K G+ +   E L +IETDK ++E  +  AG++ +I+  
Sbjct: 4   IEVKVPQLSESVAEATLLQWHKKVGEPVSRDENLIDIETDKVVLELPSPDAGVITQIIKA 63

Query: 63  AGKDIEVGAPIAVLFAEGETSVDIAAVLAAAGVGTASGTAPAAAPAPAAPFAPAPAVSAT 122
            G  +  G  IA+L  +    V              + T   AAPAP A   P P   A 
Sbjct: 64  DGATVVAGEVIAILDTDASAQV--------------APTEVKAAPAPQATNEPTPV--AA 107

Query: 123 AQRAPAGGRVFVSPLARRLARDRGIGLQGVQGSGPHGRIVKRDVDALAAATASKPVNVNA 182
            + A  G      P A +L  D  +    V G+G  GR+ K DV    +A A+ P     
Sbjct: 108 PELASKGD--VAMPAAAKLLADNNLSTSQVTGTGKDGRVTKGDVLGALSAPAAAPAAAAK 165

Query: 183 AATSTSVASSASKATDAGYVEIAHSGMRRTIARRLSESKSTIPHFYLSAACKMDKLMALR 242
            A +   A   +         +  S +R  +A RL +S+ST           M  ++ LR
Sbjct: 166 PALAQVAAPVKAGLESRPEQRVPMSRLRARVAERLVQSQSTNAILTTFNEINMQPVIDLR 225

Query: 243 AEINAGSSR----KISVNDFIVKAVAAALKASPQMNVGWTDSALRQYAQADISVAVSTDG 298
            +      +    K+    F VKAV  ALK  P +N     + +  +   DI VAV +  
Sbjct: 226 NKYKDKFEKEHGVKLGFMSFFVKAVVHALKKYPIVNASVDGNDIVYHGYFDIGVAVGSPR 285

Query: 299 GLITPVVRAADSKSLSAVSAEIADLAGFXIGAGGISGRQLHHQQSG---HVRRAGVLGDH 355
           GL+ P++R AD  S++ +  +I +      G     G+    + SG    V   G  G  
Sbjct: 286 GLVVPILRDADQMSIADIEKKIGEF-----GQKAKDGKLSIEELSGGTFTVSNGGTFGSM 340

Query: 356 QSAAGSNP 363
            S    NP
Sbjct: 341 LSTPIINP 348


>gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransferase
           component of pyruvate dehydrogenase complex
           [Herbaspirillum seropedicae SmR1]
          Length = 554

 Score = 76.6 bits (187), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 99/345 (28%), Positives = 156/345 (45%), Gaps = 37/345 (10%)

Query: 5   IRMPEIAANATSAVIKEWSRKEGDSIRIGETLAEIETDKALIEFDADQAGILGKILAPAG 64
           I +P+I       VI E   K GD+++  ++L  +E+DKA +E  +  AG++ ++    G
Sbjct: 122 IEVPDIGDFKEVEVI-EVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVKLG 180

Query: 65  KDIEVGAPIAVLFAEGETSVDIAAVLAAAGVGTASGTAPAAAPAPAAPFAPAPAVSATAQ 124
             +  G+ IA +  E       AA  AA     A+  APA A APA   A A    ATA 
Sbjct: 181 DKVSKGSIIATI--EAAGGAPAAAPAAAPAAAPAAAAAPAPASAPAPAAAAAVPAIATAS 238

Query: 125 RAPAGGRVFVSPLARRLARDRGIGLQGVQGSGPHGRIVKRDVDALAAATASKPVNVNAAA 184
               GG+   SP  R+ AR+ G+ L  V  +GP GRI++ DV             V A +
Sbjct: 239 ATSTGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKG-------VMAGS 291

Query: 185 TSTSVASSASKATDAG-----YVEIAHSGMRRTIARRLSESKS-----------TIPHFY 228
           TS + ++ A+  + AG     +  +  S    T  + LS  K             IPH  
Sbjct: 292 TSVAASAPATNGSGAGMNLLPWPSLDFSKFGETELQPLSRIKKISGPNLHRNWVMIPHVT 351

Query: 229 LSAACKMDKLMALRAEIN-----AGSSRKISVNDFIVKAVAAALKASPQMNVGW----TD 279
                 + +L   R   N     A S  K+++  F++KA  +ALK  P  N        +
Sbjct: 352 QFDEADVTELEEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGEN 411

Query: 280 SALRQYAQADISVAVSTDGGLITPVVRAADSKSLSAVSAEIADLA 324
             L+++   +I  A  T  GL+ PV++ AD KS+  ++ E+ +L+
Sbjct: 412 LILKKF--YNIGFAADTPNGLVVPVIKNADQKSIGQIAIEMGELS 454


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.129    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,019,605
Number of extensions: 37098
Number of successful extensions: 208
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 200
Number of HSP's successfully gapped: 4
Length of query: 396
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 309
Effective length of database: 1,207,925
Effective search space: 373248825
Effective search space used: 373248825
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 131 (55.1 bits)