BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0048.p (126 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 99 8e-23 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 89 1e-19 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 84 2e-18 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 83 6e-18 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 76 1e-15 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 99.4 bits (246), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 50/119 (42%), Positives = 72/119 (60%), Gaps = 1/119 (0%) Query: 1 MVKQGGGVILNIASMYGTVAAPERAAYCASKAAVVMLTKVMAVEWAPSGLRVNALAPGYV 60 M+ +G G I+NI S+ + P A Y A+K AV M TK MA++W G++VN L PGY Sbjct: 142 MIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYF 201 Query: 61 QTALVDELVRTGRMDLDALVKRTPARRLGQPHEIATLALFLASDSASYINGQAIVADGG 119 +T + LV + D LV RTP+RR G ++ A+FLASD++ ++NG + DGG Sbjct: 202 KTEMNMALVNDAKF-TDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGG 259 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 88.6 bits (218), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 51/131 (38%), Positives = 79/131 (60%), Gaps = 9/131 (6%) Query: 1 MVKQGGGVILNIASMYGTVAA-PERAAYCASKAAVVMLTKVMAVEWAPSGLRVNALAPGY 59 M+ + G I+NIAS +V R AY ASKAAVV LTK +A ++ G+R NA+ PG Sbjct: 124 MLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAICPGT 183 Query: 60 VQTALVDELV-----RTGRMDLD---ALVKRTPARRLGQPHEIATLALFLASDSASYING 111 +++ +++ + TG+ + + A V R P R+G+ E+A LAL+LASD +++ G Sbjct: 184 IESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASDESNFTTG 243 Query: 112 QAIVADGGWSS 122 + DGGWS+ Sbjct: 244 SIHMIDGGWSN 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 84.3 bits (207), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 49/120 (40%), Positives = 70/120 (58%), Gaps = 2/120 (1%) Query: 1 MVKQGGGVILNIASMYGTVAAPERAAYCASKAAVVMLTKVMAVEWAPSGLRVNALAPGYV 60 MVKQ G I+NI+S+ G A R AY SKAAV+ LT+ MA+E A G+ VN++APG V Sbjct: 131 MVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPV 190 Query: 61 QTALVDELVRTGRMDLDALVKRTPARRLGQPHEIATLALFLASDSASYINGQAIVADGGW 120 T + + ++ + P RR G EIA+ +LA++ ++YI G I DGG+ Sbjct: 191 DTPMTQAM--HSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGGF 248 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 83.2 bits (204), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 48/124 (38%), Positives = 70/124 (56%), Gaps = 9/124 (7%) Query: 1 MVKQGGGVILNIASMYGTVAAPERAAYCASKAAVVMLTKVMAVEWAPSGLRVNALAPGYV 60 +++Q G I+N ASM Y ASK V LTK +A+ +A G+RVNA+APG++ Sbjct: 120 LLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWI 179 Query: 61 QT----ALVDELVRTGRMDLDALVKRTPARRLGQPHEIATLALFLASDSASYINGQAIVA 116 T AL D+ VR G ++ RTP R GQP ++ + FL S +AS++ G + Sbjct: 180 ATPLTQALQDDPVRAG-----PILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPV 234 Query: 117 DGGW 120 DGG+ Sbjct: 235 DGGY 238 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 75.9 bits (185), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 44/113 (38%), Positives = 64/113 (56%), Gaps = 1/113 (0%) Query: 7 GVILNIASMYGTVAAPERAAYCASKAAVVMLTKVMAVEWAPSGLRVNALAPGYVQTALVD 66 G I+N+AS+ G A +AY ASK AV LT+ MA+++ G+R+NA+ P TA+ Sbjct: 140 GCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHTAMTA 199 Query: 67 ELVRTGRMDLDALVKRTPARRLGQPHEIATLALFLASDSASYINGQAIVADGG 119 ++ + LD +R R P EIA + FLAS AS++NG + DGG Sbjct: 200 DMADDPPL-LDKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 402,675 Number of extensions: 14781 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 35 Length of query: 126 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 51 Effective length of database: 1,264,745 Effective search space: 64501995 Effective search space used: 64501995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)