BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0002.p (449 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 733 0.0 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 112 6e-26 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 103 4e-23 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 102 7e-23 gi|300312669|ref|YP_003776761.1| Mg(2+) transport ATPase [Herbas... 87 2e-18 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 733 bits (1893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/444 (84%), Positives = 407/444 (91%), Gaps = 6/444 (1%) Query: 11 LTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKT 70 ++VTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKT Sbjct: 286 VSVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKT 345 Query: 71 GTITLGNRQASAFLPANGVSEQELADIAQLASLADETPEGRSIVVLAKKRFNLREREMGS 130 GTITLGNRQA+AF+PA GV+E+ELAD+AQLASLADETPEGRSIVVLAK RFNLREREM + Sbjct: 346 GTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMAN 405 Query: 131 LGAVFIPFTAQTRLSGVDIGHRQIRKGAADAIRKLAEQAGQQVPGEVAAAVDIVARRGST 190 LGA FIPF+AQTR+SG+D+GHR+IRKGAADAIRKL A Q++P +V + ++ +ARRG T Sbjct: 406 LGAEFIPFSAQTRVSGIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRGGT 465 Query: 191 PLVVAETDA------ANGSIRVLGVIELKDIVKGGIKERFSELRRMGIKTIMITGDNRLT 244 PLVV E A IR+LG +ELKDIVKGGIKERF ELRRMGIKT+MITGDNRLT Sbjct: 466 PLVVIEWHAEAAMINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLT 525 Query: 245 AAAIAAEAGVDDFLAEATPENKLSLIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNT 304 AAAIAAEAGVDDFLAEATPE KL+LIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMN+ Sbjct: 526 AAAIAAEAGVDDFLAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNS 585 Query: 305 GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFAT 364 GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMF Sbjct: 586 GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVG 645 Query: 365 TYPQLAALDVMHLNNPSSAILSAVIFNALVIVVLIPLALRGVRYRALGAAVLLRRNLLIY 424 +YPQL AL+VMHL +P SAI+SAVIFNALVIV LIPLALRGVRYRALGAAVLLRRNLLIY Sbjct: 646 SYPQLGALNVMHLASPDSAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIY 705 Query: 425 GLGGLIVPFVGIKLIDVLLGVFGL 448 G+GGL+VPFVGIKLID+LL FGL Sbjct: 706 GVGGLLVPFVGIKLIDLLLSAFGL 729 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 112 bits (280), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 124/437 (28%), Positives = 190/437 (43%), Gaps = 74/437 (16%) Query: 6 YVRGILTVTAL--IALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVD 63 Y G T L IAL + L+P + +L+ G RM +ANV+ A+E G + Sbjct: 254 YSHGRWTEALLMGIALSMSLLPEELPIILTIFPAIGAWRMARANVLTRRLSAIETLGSIS 313 Query: 64 VLLLDKTGTIT----------LGNRQASAFLPANGVSEQELADIAQLASLADET----PE 109 VL DKTGT+T + R G++ E + + A+LA P Sbjct: 314 VLCTDKTGTLTENRMRVVQGMIAGRHMYFDESDIGITSPEWRTLLETAALASSPRPTDPM 373 Query: 110 GRSIVVLA---KKRFNLREREMGSLGAVFIPFTAQTR-----LSGVDIGHRQI-RKGAAD 160 ++ LA KRF + L + PFT R G D + KGA + Sbjct: 374 EKAFHQLAGESAKRFA--PPALTQLLQEY-PFTPALRAMTMVWKGDDDAPLLVATKGAPE 430 Query: 161 AIRKLAEQAGQQVPGEVAAAVDIVARRGSTPLVVAETDAANGS-----------IRVLGV 209 AI L P EV + + GS L V A N S +R LG+ Sbjct: 431 AIASLC----GLTPAEVQQMQNDIMHLGSHGLRVIAVAACNWSGALPASQAQFGLRYLGL 486 Query: 210 IELKDIVKGGIKERFSELRRMGIKTIMITGDNRLTAAAIAAEAGVD-------------- 255 + L D ++ G+ + + I+ IMITGD+ TA IA +AG+ Sbjct: 487 LALADPLRPGVPDAIQRCHQAEIRVIMITGDHPSTAQNIARQAGISADQVLLGDEVQAMA 546 Query: 256 -----------DFLAEATPENKLSLIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNT 304 + A TP KL ++ + +G+++AMTGDG NDAPAL A V +AM Sbjct: 547 EHELARRLRTTNVCARITPLQKLRIVAALKDDGEIIAMTGDGVNDAPALKAAHVGIAMGA 606 Query: 305 -GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQML-MTRGSLTTFSIANDVAKYFAIIPAMF 362 GT A+EAG+++ LD N ++E + +G+++ RG++ + A++P +F Sbjct: 607 RGTDVAREAGSLILLDDNFASIVEGILMGRRIFSRMRGAMIYVLAMHMPIAGMALLPVVF 666 Query: 363 ATT---YP-QLAALDVM 375 YP Q+A L+++ Sbjct: 667 GWPLILYPMQIAFLELI 683 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 103 bits (256), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 90/324 (27%), Positives = 152/324 (46%), Gaps = 25/324 (7%) Query: 17 IALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLG 76 +A++V P +G +AG + ++ A+E A V+ + DKTGT+TLG Sbjct: 376 VAVMVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLTLG 435 Query: 77 NRQASAFLPANGVSEQELADIAQLASLADETPEGRSIVVLAKKRFNLRE-----REMGSL 131 + + EL +A E P GR++ A + R + L Sbjct: 436 HPRLLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSAQAARQAEQVRSVAGL 495 Query: 132 GAVFIPFTAQTRLSGVDIGHRQIRKGAADAIRKLAEQAGQQVPGEVAAAVDIVARRGSTP 191 G + GV G RQ+ G++ +++L A P A A + A+ + Sbjct: 496 G-----------IEGVVEG-RQLMLGSSRWMQQLQAHA----PALQARAEQLQAQGRTLS 539 Query: 192 LVVAETDAANGSIRVLGVIELKDIVKGGIKERFSELRRMGIKTIMITGDNRLTAAAIAAE 251 + D+ + VLG++ D +K G + + L RMG+++ M++GDN A +A+E Sbjct: 540 WLARRNDS---GVEVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDNLGAAQQVASE 596 Query: 252 AGVDDFLAEATPENKLSLIRQ-HQAEGKLVAMTGDGTNDAPALAQADVAVAMNTGTQAAK 310 G+ + AE P +K + Q ++VAM GDG NDAPALA ADV +AM+TGT A Sbjct: 597 LGIQEVRAEVLPADKAEAVTALRQGGAQVVAMVGDGVNDAPALAAADVGMAMSTGTDVAM 656 Query: 311 EAGNMVDLDSNPTKLIEIVEIGKQ 334 +A + + +P + + ++I ++ Sbjct: 657 QAAGVTLMHGDPGLVADAIDISRR 680 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 102 bits (254), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 97/332 (29%), Positives = 163/332 (49%), Gaps = 39/332 (11%) Query: 10 ILTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDK 69 +L V A LV P TI V+G++ + ++ G +E + L LDK Sbjct: 417 VLLVIACPCALVISTPVTI--------VSGLASAARHGILVKGGVYLEQGRQLRALALDK 468 Query: 70 TGTITLGNRQASAFLPANGVSEQELADIAQLA-SLADETPEGRSIVVLAKKRFNLRE-RE 127 TGT+T G + + + ++Q+ + QLA SLA + S + A++ E E Sbjct: 469 TGTLTFGKPRVTDVVSLQAQADQQA--LLQLAVSLAARSDHPVSRAIAAQEEIATVEVSE 526 Query: 128 MGSLGAVFIPFTAQTRLSG--VDIGHRQIRKGAADAIRKLAEQAGQQVPGEVAAAVDIVA 185 +L + Q R++G + +G+ +L + GQ P E+ A + Sbjct: 527 FAALAGRGV----QGRINGQLLHLGNH-----------RLVHELGQCSP-ELEARLQAFE 570 Query: 186 RRGSTPLVVAETDAANGSIRVLGVIELKDIVKGGIKERFSELRRMGIKTIMITGDNRLTA 245 ++G T V+ +G ++L + + D V+ ++ +ELR +G++ IM+TGDN TA Sbjct: 571 KQGKTTTVLC----LDGQPQLL--VAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTA 624 Query: 246 AAIAAEAGVDDFLAEATPENKLSLIRQ-HQAEG-KLVAMTGDGTNDAPALAQADVAVAMN 303 AIAA+ G+ D + PE+KL I + Q G + V M GDG NDAPALA+AD+ AM Sbjct: 625 QAIAAQTGISDARGDQLPEDKLKAIEELSQRHGVRGVGMVGDGINDAPALARADIGFAMG 684 Query: 304 -TGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 334 GT A E ++ +D + K+ + + + ++ Sbjct: 685 AAGTDTALETADVALMDDDLRKIPDFIRLSRK 716 >gi|300312669|ref|YP_003776761.1| Mg(2+) transport ATPase [Herbaspirillum seropedicae SmR1] Length = 920 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 108/411 (26%), Positives = 172/411 (41%), Gaps = 66/411 (16%) Query: 5 DYVRGILT-VTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVD 63 D+V L ++ + L ++P + L A G +SR VI A++ G +D Sbjct: 335 DWVEAFLFGLSVAVGLTPEMLPMIVTSTL-AKGAVALSR---KKVIVKRLDAIQNFGAMD 390 Query: 64 VLLLDKTGTITLGNRQASAFLPANGVSEQELAD-IAQLASLADETPEG-RSIVVLAKKRF 121 VL DKTGT+T Q FL + E D + Q A L G ++++ +A Sbjct: 391 VLCTDKTGTLT----QDKIFLERHTDIHGEPDDEVLQYAYLNSYYQTGLKNLLDVAVLEH 446 Query: 122 NLREREMGSLGAVF-----IPFTAQTRLSGVDIGHRQ-----IRKGAADAIRKLAEQA-- 169 +REM S+ + + IPF Q R V + R+ I KGA + I + +A Sbjct: 447 AELQREM-SIASAYRKVDEIPFDFQRRRMSVVVSEREDHHELICKGAVEEIVSVCSRARV 505 Query: 170 -GQQVPGEVAAAVDIVARRGS----------------TPLVVAETDAANGSIRVLGVIEL 212 G+ VP A +I A GS P + A + ++G I Sbjct: 506 NGEVVPMTPALLEEIRATTGSLNAEGLRVVGVAAKDLPPTKEVYSLADESDLVLIGYIAF 565 Query: 213 KDIVKGGIKERFSELRRMGIKTIMITGDNRLTAAAIAAEAGV---------------DDF 257 D K + LR G+K ++TGDN L A I E G+ D Sbjct: 566 LDPPKESTAPALAALRAHGVKVKILTGDNELVTAKICREVGLEVKGMLLGSYVEKMSDAE 625 Query: 258 LAEA----------TPENKLSLIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNTGTQ 307 L+EA +P +K ++R G +V GDG NDAPAL AD+ ++++T Sbjct: 626 LSEAVETVTVFAKLSPAHKERIVRVLHDRGHVVGFMGDGINDAPALRAADIGISVDTAVD 685 Query: 308 AAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAII 358 AKEA +++ L+ + L E V G++ + +++ F+++ Sbjct: 686 IAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKYIKMTASSNFGNVFSVL 736 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,227,761 Number of extensions: 48095 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 289 Number of HSP's successfully gapped: 7 Length of query: 449 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 361 Effective length of database: 1,203,190 Effective search space: 434351590 Effective search space used: 434351590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)