BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0070.0002.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 118 1e-28 gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 117 3e-28 gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 86 8e-19 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 118 bits (296), Expect = 1e-28, Method: Composition-based stats. Identities = 68/123 (55%), Positives = 92/123 (74%), Gaps = 1/123 (0%) Query: 1 VRLVLPTGYTFNLNGTNIYLTMAALFLAQAGGIEVSLWQQLSLLAICMLTSKGATSVTGS 60 V LV+PTGY+FNL+GT IYLTMAA+F+AQA ++ Q+L+LL + +LTSKGA +TGS Sbjct: 293 VGLVIPTGYSFNLDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGS 352 Query: 61 GFIALAATLSTLQIVPVGGIVLLLGVERLM-KCRSLSNVIGNCLACVVVCAWDKALDRER 119 GFI LAATLS + VPV G+ L+LG++R M + R+L+N IGN +A +VV W LD ER Sbjct: 353 GFIVLAATLSAVGHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSER 412 Query: 120 MAQ 122 + + Sbjct: 413 LTK 415 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 117 bits (294), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 58/120 (48%), Positives = 88/120 (73%), Gaps = 1/120 (0%) Query: 1 VRLVLPTGYTFNLNGTNIYLTMAALFLAQAGGIEVSLWQQLSLLAICMLTSKGATSVTGS 60 V LV+PTGY+FNL+ +IYLTMAALF+AQA +S+ ++LAI ++TSKGA V GS Sbjct: 293 VGLVIPTGYSFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGS 352 Query: 61 GFIALAATLSTLQIVPVGGIVLLLGVERLMK-CRSLSNVIGNCLACVVVCAWDKALDRER 119 + LAATL+T+ +PV G+VL+L ++ + R+L N++GNC+A VV+ +W++ +D+ R Sbjct: 353 AIVILAATLTTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWERDIDKVR 412 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 86.3 bits (212), Expect = 8e-19, Method: Composition-based stats. Identities = 46/114 (40%), Positives = 72/114 (63%), Gaps = 2/114 (1%) Query: 3 LVLPTGYTFNLNGTNIYLTMAALFLAQAGGIEVSLWQQLSLLAICMLTSKGATSVTGSGF 62 V+P GY+FNL+G+ IY T A LF+AQA GI + + Q++++ + MLTSKG V + Sbjct: 294 FVMPMGYSFNLDGSMIYCTFATLFIAQAYGIHMPISTQITMMLVLMLTSKGIAGVPRASL 353 Query: 63 IALAATLSTLQIVPVGGIVLLLGVERLMKC-RSLSNVIGNCLACVVVCAWDKAL 115 + +AATL I P G++++LG++ + RS +N +GN +A VV W+ L Sbjct: 354 VVIAATLHQFNI-PEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWEGGL 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 294,983 Number of extensions: 8244 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 37 Number of HSP's successfully gapped: 3 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)