BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0001.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 82 2e-17 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 82 2e-17 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 78 2e-16 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 75 2e-15 gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein... 70 7e-14 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 81.6 bits (200), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 46/121 (38%), Positives = 75/121 (61%), Gaps = 11/121 (9%) Query: 21 GRVVAVSSFVAHVYRLGESGFPAS---AAAKAGIEALVKSLAVQLAQSGATVNAVAPGYV 77 GR++ ++S V GE+G P AAAKAG+ + ++LA ++ TVN VAPG++ Sbjct: 138 GRIINITSVV------GEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFI 191 Query: 78 QKSDPNKSAIDPAAWAAALERIPMQRLGQPQEIANVIGFLLSPAASYVTGQVWHIDGGLT 137 +D + A++ AA L++IP+ RLG +EIA + FL SP A+Y+TG +++GG+ Sbjct: 192 -DTDMTR-ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGMY 249 Query: 138 L 138 + Sbjct: 250 M 250 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 81.6 bits (200), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 49/135 (36%), Positives = 75/135 (55%), Gaps = 16/135 (11%) Query: 10 GLTNFLSPF--------XAGRVVAVSSFVAHVYRLGESGFPASAAAKAGIEALVKSLAVQ 61 G N L+P AGR+V ++S + G+ + +AAKAG+ K+LA++ Sbjct: 118 GFYNVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNY---SAAKAGVIGATKALALE 174 Query: 62 LAQSGATVNAVAPGYVQKSDPNKSAIDPAAWAAALERIPMQRLGQPQEIANVIGFLLSPA 121 LA+ TVN VAPG + + ID L+ IP +R G+P+E+A + FL+SP Sbjct: 175 LAKRDITVNCVAPGLIDTDMTEDAPIDEI-----LKLIPARRAGKPEEVAAAVAFLMSPD 229 Query: 122 ASYVTGQVWHIDGGL 136 A+Y+T QV ++GGL Sbjct: 230 AAYITRQVISVNGGL 244 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 78.2 bits (191), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 40/94 (42%), Positives = 55/94 (58%) Query: 42 PASAAAKAGIEALVKSLAVQLAQSGATVNAVAPGYVQKSDPNKSAIDPAAWAAALERIPM 101 P +A+K G+ L KSLA+ A G VNAVAPG++ DP L R P+ Sbjct: 144 PGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRAGPILARTPL 203 Query: 102 QRLGQPQEIANVIGFLLSPAASYVTGQVWHIDGG 135 R GQP+++ V+ FL SPAAS++TG + +DGG Sbjct: 204 GRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 75.5 bits (184), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 41/118 (34%), Positives = 70/118 (59%), Gaps = 5/118 (4%) Query: 21 GRVVAVSSFVAHVYRLGESGFPASAAAKAGIEALVKSLAVQLAQSGATVNAVAPGYVQKS 80 GR++ +SS + G++ + + AKAGI +LA ++A G TVN V+PGY+ + Sbjct: 133 GRIINISSVNGQKGQFGQTNY---STAKAGIHGFTMALAQEVATKGVTVNTVSPGYI-GT 188 Query: 81 DPNKSAIDPAAWAAALERIPMQRLGQPQEIANVIGFLLSPAASYVTGQVWHIDGGLTL 138 D + AI P + IP++RLG+P+EIA+++ ++ S Y TG + ++GGL + Sbjct: 189 DMVR-AIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGLYM 245 >gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 70.1 bits (170), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 45/118 (38%), Positives = 68/118 (57%), Gaps = 11/118 (9%) Query: 21 GRVVAVSSFVAHVYRLGESGFP---ASAAAKAGIEALVKSLAVQLAQSGATVNAVAPGYV 77 GR+V ++S G+ G P A +AAKAG+ AL KSL +LA+SG VNA+AP V Sbjct: 131 GRIVNIASLA------GKEGTPNASAYSAAKAGVLALTKSLGKELAESGVLVNAIAPAAV 184 Query: 78 QKSDPNKSAIDPAAWAAALERIPMQRLGQPQEIANVIGFLLSPAASYVTGQVWHIDGG 135 + + + + PA + + PM RLG E+A++ +L S + S+ TG V+ + GG Sbjct: 185 KTALLGQ--MSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDLSGG 240 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 459,601 Number of extensions: 18137 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 22 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)