BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0068.0011.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 331 3e-92 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 111 5e-26 gi|300310642|ref|YP_003774734.1| electron transfer component of ... 82 5e-17 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 74 9e-15 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 64 1e-11 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 331 bits (849), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 163/239 (68%), Positives = 189/239 (79%), Gaps = 4/239 (1%) Query: 1 VVVRRVDESEEITSFYFAPEDGKAIMEYHPGQYIGLCVTVDGEEMRRQYSLSA----APN 56 +V R+V ESEEITSF+F P+DG ++++ PGQYIGL + VDGEE RRQYSLSA A Sbjct: 159 IVSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQ 218 Query: 57 DKMYRISVKREPNGKVSNFLHDHMTVGKQIDLMPPSGEFTLTPGERPLVLISGGVGITPM 116 YRISVKREP GKVS LHD + VG ++L PP+GEFTL ++PL LISGGVGITPM Sbjct: 219 PPQYRISVKREPGGKVSRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGITPM 278 Query: 117 LAMLDAALPSKRPVVFIHAARHGGVHAFGKKIADLAQEHPQLQHYFCYEQPREQDQAPHA 176 ++ML AALPS RP+ FIHAARHGGVHAF +++ +LA HPQLQ FCYEQPR+ D APHA Sbjct: 279 MSMLAAALPSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGDAAPHA 338 Query: 177 TGYIDRDKLAGWLPESMDVDAYFVGPKSFMKAIKRHLHELGVPAAQSRYEFFGPAASLD 235 TG I+R L WLP + D+DAYFVGPKSFMKAIKR L ELGVPAAQSRYEFFGPAASLD Sbjct: 339 TGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPAASLD 397 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 111 bits (278), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 70/233 (30%), Positives = 117/233 (50%), Gaps = 12/233 (5%) Query: 10 EEITSFYFAPEDGKAIMEYHPGQYIGLCVTVDGEEMRRQYSLSAAPNDKMYRISVKREPN 69 + I + P + + Y GQ++ L ++GE++RR YS+ +A ++ R+++KR P Sbjct: 18 DTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSICSAVQERQLRVAIKRAPG 77 Query: 70 GKVSNFLHDHMTVGKQIDLMPPSGEFT--LTPGERPLVL-ISGGVGITPMLAMLDAALPS 126 G SN+ ++ G++ID+MPP G F L G R L + G GITPM++++ L S Sbjct: 78 GLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAAGSGITPMMSIIKTTLLS 137 Query: 127 K--RPVVFIHAARHGGVHAFGKKIADLAQEHPQLQHYFCYEQPREQDQAPHATGYIDRDK 184 + ++A R F +++ DL + + + Y REQ G IDR K Sbjct: 138 EPHSHFTLVYANRASSSVIFKEELTDLKDAYLE-RFNVVYVMSREQQDVELFNGRIDRAK 196 Query: 185 ----LAGWLPESMDVDAYFV-GPKSFMKAIKRHLHELGVPAAQSRYEFFGPAA 232 A W+ + DVDA F+ GP+ ++A+ L G+P Q + E F + Sbjct: 197 CDAFFASWI-DLKDVDAAFLCGPEEMVQAVAASLQAHGLPKTQIKTELFAAST 248 >gi|300310642|ref|YP_003774734.1| electron transfer component of chlorobenzoate 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 81.6 bits (200), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 62/214 (28%), Positives = 97/214 (45%), Gaps = 14/214 (6%) Query: 18 APEDGKAIMEYHPGQYIGLCVTVDGEEMRRQYSLSAAPNDKMYRISVKREPNGKVSNFLH 77 AP + A + PGQY+ + V G R YS S+ +K P G +SN+L Sbjct: 129 APANAPAFL---PGQYVN--IGVPGSGQHRSYSFSSPSGASRMSFLIKNVPGGLMSNWLA 183 Query: 78 DHMTVGKQIDLMPPSGEFTLTPGERPLVLISGGVGITPMLAMLD--AALPSKRPVVFIHA 135 + G ++L P G F L P +RP+++++GG G+ P L+ML+ A +PV I+ Sbjct: 184 GA-SEGATLELTGPLGSFYLRPVKRPVLMLAGGTGLAPFLSMLEELAQQGLDQPVHLIYG 242 Query: 136 ARHGGVHAFGKKIADLAQEHPQLQHYFCYEQPREQDQAPHA-TGYIDRDKLAGWLPESMD 194 +++ LA P C P HA GY+ + A L + + Sbjct: 243 VTRDQDLVLVERLQALADRLPGFSFTTCVADPATT----HARQGYVTQHMPAAVLHDG-N 297 Query: 195 VDAYFVGPKSFMKAIKRHLHELGVPAAQSRYEFF 228 VD Y GP ++A+++H G+ A YE F Sbjct: 298 VDVYLCGPPPMVEAVQKHFQATGMQPASFYYEKF 331 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 74.3 bits (181), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 63/232 (27%), Positives = 107/232 (46%), Gaps = 18/232 (7%) Query: 2 VVRRVDESEEITSFYFAPEDGKAIMEYHPGQYIGLCVTVDGEEMRRQYSLSAAPNDK-MY 60 V R +E+ +I + DG A+ + G +I V + G + RQYSL AP+++ Y Sbjct: 8 VAARREEAHDIIALELVAVDGAALPAFTAGAHID--VHLPGG-LVRQYSLCNAPHEQHRY 64 Query: 61 RISVKREPNGKVSNF-LHDHMTVGKQIDLMPPSGEFTLTPGERPLVLISGGVGITPMLAM 119 +I V R+ N + + +H+ + +G + + P +F L P + L L++GG+G+TP+L M Sbjct: 65 QIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALVPAQHSL-LLAGGIGVTPILCM 123 Query: 120 LDAALPSKRPVVFIHAARHGGVHAFGKKIADLAQEHPQLQHYFCYEQPREQDQAPHATGY 179 +A + + AR AF ++IA + HY E ++ Sbjct: 124 AEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQK---------- 173 Query: 180 IDRDKLAGWLPESMDVDAYFVGPKSFMKAIKRHLHELGVPAAQSRYEFFGPA 231 +D D L + + Y GP F+ + G P AQ +E+FG A Sbjct: 174 LDLDALLAQADPATHL--YVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAA 223 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 43/120 (35%), Positives = 65/120 (54%), Gaps = 6/120 (5%) Query: 3 VRRVDES-EEITSFYFAPEDGKAIMEYHPGQYIGLCVTVDGEEMRRQYSLSAAPNDK-MY 60 VR V ++ ++I +DG A+ + PG ++ L + DG + R YSL P + Y Sbjct: 20 VRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQ-DG--LVRAYSLCNTPQQRDCY 76 Query: 61 RISVKREPNGKV-SNFLHDHMTVGKQIDLMPPSGEFTLTPGERPLVLISGGVGITPMLAM 119 I+VKREP+ + S FLH+ + G I PP F L R +L++ G+GITP+ AM Sbjct: 77 LIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHAM 136 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 828,309 Number of extensions: 35996 Number of successful extensions: 113 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 7 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)