BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0068.0005.p (102 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309491|ref|YP_003773583.1| cytoplasmic protein [Herbaspiri... 74 2e-15 >gi|300309491|ref|YP_003773583.1| cytoplasmic protein [Herbaspirillum seropedicae SmR1] Length = 153 Score = 74.3 bits (181), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 44/143 (30%), Positives = 58/143 (40%), Gaps = 46/143 (32%) Query: 1 MEQRVDFYKASPEVLKGMIALENVVGKLGLETSLLELVRLRVSLINGCAFCVDMHTADAR 60 +QR+ K + +I VV + G++ LLELV LRVS +NGCAFCVDMH+ D Sbjct: 4 FKQRLPHAKLAAPAFNALIKASEVVHQSGVDPKLLELVFLRVSQLNGCAFCVDMHSHDLL 63 Query: 61 KAGETEAR----------------------------------------------AQFTDA 74 + GE + R F D Sbjct: 64 QKGEDQQRLNTLAAWREIPFFTDAERAALNWAERFTHLHQQDADKEDAAFVQLQQHFNDT 123 Query: 75 ELVNLTLAINTINAWNRFAIGFR 97 E+ NLT A+ INAWNR + R Sbjct: 124 EIANLTFAVAIINAWNRLGVSMR 146 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 228,867 Number of extensions: 5286 Number of successful extensions: 20 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 20 Number of HSP's successfully gapped: 1 Length of query: 102 Length of database: 1,619,870 Length adjustment: 71 Effective length of query: 31 Effective length of database: 1,283,685 Effective search space: 39794235 Effective search space used: 39794235 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 123 (52.0 bits)