BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0067.0012.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314026|ref|YP_003778118.1| hypothetical protein Hsero_4749... 184 3e-48 >gi|300314026|ref|YP_003778118.1| hypothetical protein Hsero_4749 [Herbaspirillum seropedicae SmR1] Length = 154 Score = 184 bits (466), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 83/125 (66%), Positives = 103/125 (82%) Query: 19 TARADTLKSEAEVRQLADKVMAAAGKGQTDDAYAAMTPYTLTDVNTLDRARLTARTSRMQ 78 +A+ADTLK+EAEVR LADKVMA AG G+TDDAY ++PY+L D+ L+ AR AR+SRM Sbjct: 28 SAQADTLKTEAEVRALADKVMAEAGAGRTDDAYGLLSPYSLVDIRALENARTQARSSRMA 87 Query: 79 LEQTIGGSVGYEFIRSEKVGESLLKLVYIEKAEKQAIPWQFIFYKTASGWALSNFSNGDN 138 +E +G SVGYEFIRSEKVG+SLLKL YIEK E+QAIPW FIFYK +GWA+S FSNG + Sbjct: 88 IEALVGNSVGYEFIRSEKVGQSLLKLTYIEKTERQAIPWMFIFYKAPTGWAVSTFSNGAD 147 Query: 139 INSLF 143 +++LF Sbjct: 148 VDALF 152 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.128 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 319,337 Number of extensions: 10082 Number of successful extensions: 38 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 38 Number of HSP's successfully gapped: 1 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)