BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0066.0157.p
         (337 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311593|ref|YP_003775685.1| ATP-dependent DNA ligase [Herba...   131   6e-32

>gi|300311593|ref|YP_003775685.1| ATP-dependent DNA ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 555

 Score =  131 bits (330), Expect = 6e-32,   Method: Compositional matrix adjust.
 Identities = 64/95 (67%), Positives = 72/95 (75%)

Query: 211 VAPACAKTVAQRLVGWTDRGTRPTAAGFAELIAPPSEADRQRRGGQPYPFFLAHPLLADP 270
           VA   AK VAQR+VGWTD   RP A  FA LIAP   AD+  +GGQPYPFFLAHPL+  P
Sbjct: 163 VAQLDAKLVAQRMVGWTDGRARPDAERFARLIAPADSADQPLQGGQPYPFFLAHPLMDAP 222

Query: 271 ATLGDPGEWQIEWKYDGIRAQLVHREGRSWLWSRG 305
           ATLG   +WQ+EWKYDGIRAQLV R G++WLWSRG
Sbjct: 223 ATLGPIQQWQVEWKYDGIRAQLVRRHGQNWLWSRG 257



 Score =  114 bits (285), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 72/115 (62%), Positives = 77/115 (66%), Gaps = 6/115 (5%)

Query: 1   VVIDGEIVIRRGGGSDGNEGVIAPFADLQKRIGRKTLSARLLADLPAALIAYDLLELDGQ 60
            VIDGEIVIR        E V APF++LQKRIGRK LSARLLA+LPA LIAYDLLE DG 
Sbjct: 277 TVIDGEIVIRHPDAGLAAERV-APFSELQKRIGRKNLSARLLAELPAGLIAYDLLEQDGC 335

Query: 61  DLRGRPQQERRALLEKLAADVP--GLTLAPVIGPAEAADWSALATLRESARARGL 113
           DLRG PQ ERR  LE L    P   L LAP +    A DW ALA LR SAR RG+
Sbjct: 336 DLRGLPQSERRRRLEALVGSHPAAALMLAPTL---TAKDWDALAQLRASARERGV 387


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,263,395
Number of extensions: 58545
Number of successful extensions: 215
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 213
Number of HSP's successfully gapped: 2
Length of query: 337
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 251
Effective length of database: 1,212,660
Effective search space: 304377660
Effective search space used: 304377660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)