BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0155.p (91 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311593|ref|YP_003775685.1| ATP-dependent DNA ligase [Herba... 124 1e-30 >gi|300311593|ref|YP_003775685.1| ATP-dependent DNA ligase [Herbaspirillum seropedicae SmR1] Length = 555 Score = 124 bits (312), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 61/79 (77%), Positives = 71/79 (89%) Query: 1 LTDAEIARVDRIIRSSTVEKFGPVRSVKPSMVFEIGFEGIAASPRHKAGIAVRFPRILRI 60 LTDAEIA VD++IR ST+EKFGPVRSV+PSMVFEIGFEGIA S RHKAG+AVRFPRILR Sbjct: 468 LTDAEIAVVDQVIRKSTIEKFGPVRSVQPSMVFEIGFEGIALSSRHKAGMAVRFPRILRQ 527 Query: 61 RDDKPIAEADSLSTLQALL 79 R DK + +AD+L+TL+ALL Sbjct: 528 RLDKTVEQADTLATLRALL 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 258,126 Number of extensions: 8966 Number of successful extensions: 24 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 24 Number of HSP's successfully gapped: 1 Length of query: 91 Length of database: 1,619,870 Length adjustment: 61 Effective length of query: 30 Effective length of database: 1,331,035 Effective search space: 39931050 Effective search space used: 39931050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)