BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0142.p (636 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 330 2e-91 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 302 6e-83 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 273 5e-74 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 270 2e-73 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 140 4e-34 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 330 bits (847), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 226/647 (34%), Positives = 325/647 (50%), Gaps = 48/647 (7%) Query: 26 DYAIYMLDPQGKVTSWNKGAQQFTGYSSE-DILGS--HFSVFYPLPEREAGLPQXALRDG 82 D + +D G +T N Q GY++E D+LG H + P E ++ LR Sbjct: 451 DQGFFSIDSAGIITLCNAAFLQLLGYAAEKDVLGRPIHDVILQPSEETQSRTGCAVLRAA 510 Query: 83 K------FEDEGWRLRKDGTTYWASSIIDPIIADDGELLGFAKITRDITEHKLAAQTLQA 136 + + E + LR DGT++ P+ DG+L G D+TE + Q L Sbjct: 511 REGVSAHVDSENF-LRVDGTSFPVQYQTHPVW-QDGQLQGALCTFVDLTERRRGLQALSW 568 Query: 137 SEQQFRMLVQGVTDYAIYMLSPEGKVTNWNAGAQRIKGYTAEEVIGTHFSNFYTETD--R 194 SE + R + + L +G+ N R+ G + EE++G ++ D Sbjct: 569 SENRLRAIFNQAS-VCFSELDLQGRYLRVNQALCRMLGRSEEEMLGMTLADVTHPDDVPM 627 Query: 195 NDGLPERALMTAAEQGR-YEQEGLRVRKDGSTFWAHVIVDAIRSELGDLIGFAKVTRDVT 253 N L R + E GR + E +R DGSTFW + I G V+ D+T Sbjct: 628 NMSLFNRCI----EDGRSFTLEKRYLRPDGSTFWVSSDISRIVDGEGRPQAMIAVSTDIT 683 Query: 254 ERR------------------ETALALARANAALFQSQKMEALGQLTGGVAHDFNNLLAV 295 ERR E L +RA AAL Q QKMEA+GQLTGGVAHDFNN+L + Sbjct: 684 ERRRAEEALRELNDTLEQRVVEEILERSRAEAALRQMQKMEAVGQLTGGVAHDFNNVLQI 743 Query: 296 ASSGLQLLTVQFPELENSRSMESIRRAL---SRGAILTQQLLSFARQQPLQPETHEVNTL 352 LQLL P L + RS + +R A+ RGA L+ QLL+FAR+QPLQP + + + Sbjct: 744 IGGNLQLLQSD-PRL-DERSRQRVRIAIGAVDRGAKLSSQLLAFARRQPLQPRSINLERV 801 Query: 353 IADFESVLRRAGNSSIQFDIRKHPTPVMVRLDATRFETSLLNLVVNATQAMPGGGKVTIA 412 + + + +LRRA +I R LD + E ++N +NA AMP GG++T+ Sbjct: 802 VQNMDDLLRRAVGENIDISTRVAHDLWNTMLDPNQVENLIINTAINARDAMPSGGRLTVQ 861 Query: 413 TDKLTLRDKAV---GTLPSGVYAKISVTDNGSGMTPEVQARVFEPFFTTKEAGQGTGLGL 469 D + L D L G Y ++ ++DNG+GM+PEV+ R FEPFFTTK G+GTGLGL Sbjct: 862 IDNVRLEDPVFLSQADLAPGEYVELQISDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGL 921 Query: 470 SQVYGFITQSGGEVTLSSEVGVGTCFTIYIPVDTDADAENAQNAD---KQRTETVLIVDD 526 S YGF+ QS G +++ SE G GT IY P D + + D ET+L+V+D Sbjct: 922 SMAYGFVKQSKGHISIHSEQGKGTTIRIYFPRCLDEEVRIDEVRDLTVHGGDETILVVED 981 Query: 527 QLDLLDITAELFRTMGYEAYTATNGAEALEVLERAPQIDILFTDVIMPNSISGIELAQIT 586 +L + ++GY A NG ALE+L+ ID++FTDV+MP S+ ELAQ Sbjct: 982 DPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTELAQQA 1041 Query: 587 RQRHPTTKIILASGHPFPALTSNQQLSKEFAFLNKPYQLADLARILR 633 R P +I+ SG+P + +L L+KPY++ LA+ +R Sbjct: 1042 RLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIR 1088 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 41/116 (35%), Positives = 62/116 (53%), Gaps = 13/116 (11%) Query: 521 VLIVDDQLDLLDITAELFRTMGYEAYTATNGAEALEVLERAPQIDILFTDVIMPNSISGI 580 +L+V+D +D + EL T+G++A +G EAL+ LE D+LFTDV +P +SG+ Sbjct: 1166 LLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQ-GFDVLFTDVNLPG-MSGV 1223 Query: 581 ELAQITRQRHPTTKIILASGHPFPALTSNQQLSKEFAF----LNKPYQLADLARIL 632 ELA+ R P +I ASG+ +++ F L KPY + L IL Sbjct: 1224 ELAKAALGRFPDLAVIFASGY-------GTMVARNVGFAAHALPKPYDIDQLQEIL 1272 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 302 bits (773), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 184/491 (37%), Positives = 286/491 (58%), Gaps = 27/491 (5%) Query: 158 PEGKVTNWNAGAQRIKGYTAEEVIGTHFSNFY-TETDRNDGLPERALMTAAEQGRYEQEG 216 P+ + N + GY++ EV+G + ETDR+ + AE+ E Sbjct: 64 PDNPIVFVNNAFINMTGYSSAEVVGKNCRFLQGPETDRS--VVAEVRRAVAERREIATEL 121 Query: 217 LRVRKDGSTFWAHVIVDAIRSELGDLIGFAKVTRDVTERRETALALARANAALFQSQKME 276 L RK+GSTFW + + + + G+L F D++ RR+ A AL Q++KME Sbjct: 122 LNYRKNGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRD-------AEQALGQARKME 174 Query: 277 ALGQLTGGVAHDFNNLLAVASSGLQLLTVQF----PELEN-SRSMESIRRALSRGAILTQ 331 ALGQLTGG+AHDFNNLL V + L ++ + P+L +RS +SIR+A + A+LTQ Sbjct: 175 ALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLTQ 234 Query: 332 QLLSFARQQPLQPETHEVNTLIADFESVLRR--AGNSSIQFDIRKHPTPVMVRLDATRFE 389 QLL+FAR+Q L+ T +N+L F ++ R G+ ++Q ++ + RLD T+ E Sbjct: 235 QLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNC--RLDPTQME 292 Query: 390 TSLLNLVVNATQAMPGGGKVTI--ATDKLTLRDKAVGT-LPSGVYAKISVTDNGSGMTPE 446 +LLN+++NA AM G G+V I A + L D+AV L G+Y I+VTD+G G+ P+ Sbjct: 293 VALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPD 352 Query: 447 VQARVFEPFFTTKEAGQGTGLGLSQVYGFITQSGGEVTLSSEVGVGTCFTIYIPVDTDAD 506 + RV +PFFTTK+ G+GTGLGLS VYGF+ QSGG V ++S GT TIY P D Sbjct: 353 ILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQDQV 412 Query: 507 AENAQNADKQRT----ETVLIVDDQLDLLDITAELFRTMGYEAYTATNGAEALEVLERAP 562 + ++ ++ E +L+VDD+L++ ++ + ++GY + AEAL+++ Sbjct: 413 GGGFEYDNRSQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEALQLIRSGR 472 Query: 563 QIDILFTDVIMPNSISGIELAQITRQRHPTTKIILASGHPFPALTSNQQLSKEFAFLNKP 622 +D+LFTD+IMP S++G+ LA+ ++ PT K++L +G+ +L EF L+KP Sbjct: 473 PVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESL-ERHGADGEFPVLDKP 531 Query: 623 YQLADLARILR 633 Y+ +LAR +R Sbjct: 532 YRHDELARKIR 542 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 273 bits (697), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 161/379 (42%), Positives = 221/379 (58%), Gaps = 12/379 (3%) Query: 268 ALFQSQKMEALGQLTGGVAHDFNNLLAVASSGLQLLTVQFPE---LENSRSMESIRRALS 324 AL SQK+EA+GQLTGG+AHDFNNLL S L L+ V+ + E R +E R+ Sbjct: 458 ALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRGEELLRYVELAARSTQ 517 Query: 325 RGAILTQQLLSFARQQPLQPETHEVNTLIADFESVLRRAGNSSIQFDIRKHPTPVMVRLD 384 R A LTQ+LL+FAR+Q L P+ VN LI+ E ++RR +I ++ +D Sbjct: 518 RAASLTQRLLAFARRQTLAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWPTFVD 577 Query: 385 ATRFETSLLNLVVNATQAMPGGGKVTIATDKLTLRDKAVGT--LPSGVYAKISVTDNGSG 442 + E++LLNL +NA AMPGGG++TI T L D+ LP G Y ISVTD+G G Sbjct: 578 GPQLESALLNLCINARDAMPGGGRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVG 637 Query: 443 MTPEVQARVFEPFFTTKEAGQGTGLGLSQVYGFITQSGGEVTLSSEVGVGTCFTIYIPV- 501 M P+V AR F+PFFTTK G GTGLGLS VYGF QSGG+V L SEVG GT +IY+P Sbjct: 638 MPPDVLARAFDPFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYLPRH 697 Query: 502 --DTD----ADAENAQNADKQRTETVLIVDDQLDLLDITAELFRTMGYEAYTATNGAEAL 555 D D ADA + A E VL+VDD+ + + AE+ GY+ A +G AL Sbjct: 698 QHDGDEQDEADALDETAAGDGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAEDGPGAL 757 Query: 556 EVLERAPQIDILFTDVIMPNSISGIELAQITRQRHPTTKIILASGHPFPALTSNQQLSKE 615 +L+ +ID+L TDV +P ++G ++A R P ++ +G+ A N L + Sbjct: 758 RILQSKQRIDLLITDVGLPGGLNGRQVADAGRAARPDLHVLFITGYAENAAFDNGHLERG 817 Query: 616 FAFLNKPYQLADLARILRS 634 + KP+++ LAR ++S Sbjct: 818 MQLMTKPFKMEALARRVQS 836 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 270 bits (690), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 173/476 (36%), Positives = 253/476 (53%), Gaps = 47/476 (9%) Query: 200 ERALMTA------AEQGRYEQEGLRVRKDGSTFW------AHVIVDAIRSELGDLIGFAK 247 +R L++A A G Y + R+DG +W + D I S+ ++ + Sbjct: 201 DRELLSARIREALARGGAYAHQYRTRRRDGRYYWLEANGRVEMTPDGIPSKFPGVLIDIE 260 Query: 248 VTRDVTERRETALALAR-------------------ANAALFQSQKMEALGQLTGGVAHD 288 R V R+ ALA R A AL Q+QKMEA+GQLTGG+AHD Sbjct: 261 GRRAVEAERDRALAALRTLNETLEQRVEERTAALLNAEEALRQAQKMEAVGQLTGGLAHD 320 Query: 289 FNNLLAVASSGLQLLTVQFPEL---ENSRSMESIRRALSRGAILTQQLLSFARQQPLQPE 345 FNN+LA S L+L+ V+ + + R + + A+ R A LTQ+LL+F+R+Q L P+ Sbjct: 321 FNNILAGISGSLELMKVRLAQGRIGDIERHLNGAQSAVKRAAALTQRLLAFSRRQTLDPK 380 Query: 346 THEVNTLIADFESVLRRAGNSSIQFDIRKHPTPVMVRLDATRFETSLLNLVVNATQAMPG 405 ++N ++A ++ R+ +I + +D + E +LLNL +NA AMP Sbjct: 381 PADLNRIVAGMHELIGRSVGPAIAVETIAAGGLWHTFVDIGQMENALLNLCINARDAMPH 440 Query: 406 GGKVTIATDKLTLRDKAVGTL----PSGVYAKISVTDNGSGMTPEVQARVFEPFFTTKEA 461 GG++TI T L D +G L P G Y +SV+D G+GM+PEV AR F+PFFTTK Sbjct: 441 GGRLTIETANRWLDD--IGALQRGVPPGQYVSLSVSDTGAGMSPEVVARAFDPFFTTKPT 498 Query: 462 GQGTGLGLSQVYGFITQSGGEVTLSSEVGVGTCFTIYIP-------VDTDADAENAQNAD 514 GQGTGLGLS VYGF QSGG V + SE+G G IY+P VD A E + Sbjct: 499 GQGTGLGLSMVYGFAGQSGGTVRIYSELGQGAMVCIYLPRHVGEGGVDDLALPEESHVPA 558 Query: 515 KQRTETVLIVDDQLDLLDITAELFRTMGYEAYTATNGAEALEVLERAPQIDILFTDVIMP 574 ++T+L+VDD+ + +T E+ R +GY A +G AL V+E +ID+L TDV +P Sbjct: 559 APGSKTILVVDDEPLVRMVTVEVLRDLGYSVLEAEDGPSALRVVEACQEIDLLLTDVGLP 618 Query: 575 NSISGIELAQITRQRHPTTKIILASGHPFPALTSNQQLSKEFAFLNKPYQLADLAR 630 N ++G +LA R P ++ +G+ A+ ++ L + L KP+ LAR Sbjct: 619 NGMNGRQLADAIRAPRPDLPVLFVTGYAENAVLNHGHLERGMQVLTKPFAADVLAR 674 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 140 bits (352), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 118/419 (28%), Positives = 184/419 (43%), Gaps = 72/419 (17%) Query: 140 QFRMLVQGVTDYAIYMLSPEG--KVTNWNAGAQRIKGYTAEEVIG--THFSNFYTETDRN 195 ++R ++ G T IY P G ++T + A R+ GY E++ + N D Sbjct: 34 RYRHII-GNTPAIIYSSVPSGDFRLTFVSENASRVLGYEPCEMLDDPNFWFNHIHPDDTA 92 Query: 196 DGLPERALMTAAEQGRYEQEGLRVR-KDGSTFWAHVIVDAIRSELGDLIGFAKVTRDVTE 254 A++ +Q YE R R +GS W H + IR G + D+T Sbjct: 93 QIFSSLAMLFVEDQKIYE---YRFRTSNGSYLWMHDTLRLIRDPDGTPQEVIGLMTDITG 149 Query: 255 RRETALALAR--------------ANAALFQSQKMEALGQLTGGVAHDFNNLLAVASSGL 300 R+E L R A A L QS+K+ ++GQL GVAH+ NN + S+ L Sbjct: 150 RKEMEDTLQRQAEEQRKLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANL 209 Query: 301 QLLTVQFPEL-ENSRSMESIRRALSRGAILTQQLLSFARQQPLQPETHEVNTLIADFESV 359 L L E + + ++ A+ +QL SF + L + +V L+A+ Sbjct: 210 NTLGNYVATLLEGIQLHQCLQGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNEG 269 Query: 360 LRRA---------------------------------GNSSIQFDI---RKHPTPVMVRL 383 L+R N+ +++ + +++ +V+ Sbjct: 270 LQRVRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIVKQYGELPLVKC 329 Query: 384 DATRFETSLLNLVVNATQAMPGGGKVTIATDKLTLRDKAVGTLPSGVYAKISVTDNGSGM 443 A+ +NL+VNA QA+ G VTI T + +G +S++D G G+ Sbjct: 330 LASELNQVFMNLLVNAAQAISERGTVTICTAR------------AGDKVSVSISDTGHGI 377 Query: 444 TPEVQARVFEPFFTTKEAGQGTGLGLSQVYGFITQSGGEVTLSSEVGVGTCFTIYIPVD 502 PE R+FEPFFTTK G GTGLGLS YG + + GG++ +SS G GT FTI +P+D Sbjct: 378 APENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPID 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,783,750 Number of extensions: 70800 Number of successful extensions: 436 Number of sequences better than 1.0e-08: 28 Number of HSP's gapped: 403 Number of HSP's successfully gapped: 30 Length of query: 636 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 545 Effective length of database: 1,188,985 Effective search space: 647996825 Effective search space used: 647996825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)