BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0141.p (101 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313911|ref|YP_003778003.1| cations and cationic drugs memb... 57 3e-10 >gi|300313911|ref|YP_003778003.1| cations and cationic drugs membrane transport protein [Herbaspirillum seropedicae SmR1] Length = 110 Score = 57.0 bits (136), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 38/101 (37%), Positives = 59/101 (58%) Query: 1 LSIAGVLEIGFAFGMKASAGFTRLIPSLLTVATGLSSVILLSLSLRTLPVGTGYAVWTGI 60 L+IA V E+ +K+S GFTRL+P L+ VA S L+L+LRT+P YA+W+G+ Sbjct: 7 LAIAIVAEVIATTALKSSQGFTRLLPGLIVVAGYGVSFYCLALTLRTMPTSVAYAIWSGV 66 Query: 61 GAAGTALLGMVLLGDSIAPLRLLCIALILAGVIGLKLVATS 101 G L ++ + + IALI+AGV+ + L ++S Sbjct: 67 GLVLITLAAWLIHHQRLDWPAVAGIALIVAGVMVINLFSSS 107 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 231,639 Number of extensions: 5765 Number of successful extensions: 66 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 65 Number of HSP's successfully gapped: 1 Length of query: 101 Length of database: 1,619,870 Length adjustment: 70 Effective length of query: 31 Effective length of database: 1,288,420 Effective search space: 39941020 Effective search space used: 39941020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 123 (52.0 bits)