BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0140.p (152 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309491|ref|YP_003773583.1| cytoplasmic protein [Herbaspiri... 110 7e-26 >gi|300309491|ref|YP_003773583.1| cytoplasmic protein [Herbaspirillum seropedicae SmR1] Length = 153 Score = 110 bits (274), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 54/143 (37%), Positives = 84/143 (58%), Gaps = 3/143 (2%) Query: 3 KRIDYANASPETYKAFGGVYSSLLKGSLPKALIDLVYLRVSQINGCAFCIDMHTRDLLKS 62 +R+ +A + + A + + + L++LV+LRVSQ+NGCAFC+DMH+ DLL+ Sbjct: 6 QRLPHAKLAAPAFNALIKASEVVHQSGVDPKLLELVFLRVSQLNGCAFCVDMHSHDLLQK 65 Query: 63 GLAIDKLVLVPVWHEGGALFSNRERAALAWAESVTRVAETGV--PDADYAAAAAEFNAKE 120 G +L + W E F++ ERAAL WAE T + + DA + FN E Sbjct: 66 GEDQQRLNTLAAWRE-IPFFTDAERAALNWAERFTHLHQQDADKEDAAFVQLQQHFNDTE 124 Query: 121 LADLTYAIGLMNAFNRFGVAFRP 143 +A+LT+A+ ++NA+NR GV+ RP Sbjct: 125 IANLTFAVAIINAWNRLGVSMRP 147 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 377,954 Number of extensions: 12184 Number of successful extensions: 39 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 37 Number of HSP's successfully gapped: 1 Length of query: 152 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 75 Effective length of database: 1,255,275 Effective search space: 94145625 Effective search space used: 94145625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)