BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0137.p (423 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 499 e-142 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 464 e-132 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 418 e-118 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 324 7e-90 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 319 3e-88 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 499 bits (1284), Expect = e-142, Method: Compositional matrix adjust. Identities = 235/413 (56%), Positives = 305/413 (73%), Gaps = 14/413 (3%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDVAHLKQARGSGIMYCWIDVEQGK 62 +LKKIKV++L LGMYFCGFEAAWLDHPFWKN+FLLK+ + L+QA SGI YCWID E+G Sbjct: 1 MLKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGL 60 Query: 63 DVAVEEPEQQEAEPEAGPESGAEPVSAEAAPGLRDELPVAIGADPFFEELEQAQRLCENA 122 DVA EE A P E EP A AAP P L A L E A Sbjct: 61 DVAQEEEAADSAAPLQETEPSPEPEHA-AAP-------------PIDAVLLDAMALRETA 106 Query: 123 KDTTKAMFADIRMGRVIDMDRCVDLVDQVTASIMRNSAAMTSIVRLKLADDYTYMHSVAV 182 K TKAMF++ RMG+V+DM+RCV +V+++ S++++ S++RLKLAD+YTY+HSVAV Sbjct: 107 KKATKAMFSEARMGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAV 166 Query: 183 CTLMVALGRTIGMDEPACKEAGLAGLLHDVGKAFIPVPILHKAGGLTPAELDIVRRHPVL 242 C LMVALGRT+G+DE AC+EAGL G LHDVGKAFIP +L+K G L+ E +++HP L Sbjct: 167 CALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPEL 226 Query: 243 GYNHLIKNPNIPQYAIEVCLHHHEKIDGTGYPDNLSGAGISLLSRMASICDVYDAVTSDR 302 GY +L ++PN+P YA +VCLHHHEKIDG GYP+ L I+L SRMA+ICDVYDA+TSDR Sbjct: 227 GYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDR 286 Query: 303 PYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQSAGG 362 PYK+ WDPAE+++AMASW+GHFD+ + AFVKTLGIYP+GS++K+ESGR G VIRQ+A Sbjct: 287 PYKRAWDPAEAISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQD 346 Query: 363 LTKPVVKAFRYDNNGDIIDSEIIDLSDPAASDSILQRGGEEWLKYNGLNKLWM 415 LT+PV+K F YD++ + D+EI+DL +D+I+ RGGEEW + + L+ + + Sbjct: 347 LTRPVLKVFHYDDSMTVTDTEILDLLANPEADTIVARGGEEWARLSHLDAMQL 399 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 464 bits (1195), Expect = e-132, Method: Compositional matrix adjust. Identities = 221/410 (53%), Positives = 291/410 (70%), Gaps = 22/410 (5%) Query: 1 MSLLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDVAHLKQARGSGIMYCWIDVEQ 60 M+ LKKI+V +L +GMYF GF+ AW+D PFW+ FL++ + + +AR +G YCWID +Q Sbjct: 1 MTRLKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQ 60 Query: 61 GKDVAVEEPEQQEAEPEAGPESGAEPVSAEAAPGLRDELPVAIGADPFFEELEQAQRLCE 120 G D+A AE + PE+ E P A G+ EL+ A RL E Sbjct: 61 GIDLA-------GAEADRLPET---------------EEPEAAGSSSSRSELDDALRLYE 98 Query: 121 NAKDTTKAMFADIRMGRVIDMDRCVDLVDQVTASIMRNSAAMTSIVRLKLADDYTYMHSV 180 NAK TT+ MF R+G+ +D++ C LV+++ S+ R+S A+ SI+RLK D+YTY+HS+ Sbjct: 99 NAKQTTQQMFKQARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSI 158 Query: 181 AVCTLMVALGRTIGMDEPACKEAGLAGLLHDVGKAFIPVPILHKAGGLTPAELDIVRRHP 240 AVCTLMVAL RT+GMD+ ACKEAGLAG LHDVGKAFIP+ IL+K L+ E I++RHP Sbjct: 159 AVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHP 218 Query: 241 VLGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGYPDNLSGAGISLLSRMASICDVYDAVTS 300 LGY++L +P Y ++VCLHHHEKIDG+GYP G IS+ SRMA++CDVYDAVTS Sbjct: 219 ALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTS 278 Query: 301 DRPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQSA 360 +RPYK GWDPAESL+ MASW GHFD+ + AFVK LGIYPIGS++K++SGR LVIRQ+ Sbjct: 279 ERPYKSGWDPAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNH 338 Query: 361 GGLTKPVVKAFRYDNNGDIIDSEIIDLSDPAASDSILQRGGEEWLKYNGL 410 LTKPVVKAF + ++++SEIIDLS P +D+I +R G++WLK NGL Sbjct: 339 VDLTKPVVKAFVTTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGL 388 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 418 bits (1074), Expect = e-118, Method: Compositional matrix adjust. Identities = 203/404 (50%), Positives = 277/404 (68%), Gaps = 17/404 (4%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDVAHLKQARGSGIMYCWIDVEQGK 62 +LKKIKV +L LGMYFCGFEA+WL HPFWK RFLL + L+QAR SGI YCWIDV +G+ Sbjct: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGR 60 Query: 63 DVAVEEPEQQEAEPEAGPESGAEPVSAEAAPGLRDELPVAIGADPFFEELEQAQRLCENA 122 DVA P + EA + A GAD + A L E+A Sbjct: 61 DVAGAAPAKAEAAAAPASPDQSAATHA--------------GADA---DRASAVALREHA 103 Query: 123 KDTTKAMFADIRMGRVIDMDRCVDLVDQVTASIMRNSAAMTSIVRLKLADDYTYMHSVAV 182 ++ T+ MF RMG+++++ +CV LV+++ S++R+ + S++RLKL D+YTY+HSV+V Sbjct: 104 REVTRQMFNAARMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSV 163 Query: 183 CTLMVALGRTIGMDEPACKEAGLAGLLHDVGKAFIPVPILHKAGGLTPAELDIVRRHPVL 242 C L++ LGRT+G+ E AC+EAGL+G LHDVGKAFI IL+K G LT E +++RHP L Sbjct: 164 CALVIGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPEL 223 Query: 243 GYNHLIKNPNIPQYAIEVCLHHHEKIDGTGYPDNLSGAGISLLSRMASICDVYDAVTSDR 302 GY +LI+ P++P YA +VC HHHE++DG GYPD L I+L +RM ++CDVYDA+TS+R Sbjct: 224 GYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSER 283 Query: 303 PYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQSAGG 362 PYK GWDPAE+++ MA W GHFD+ LL AFVKTLGIYP+GSL+++ESG G+VIRQ+ Sbjct: 284 PYKHGWDPAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPAD 343 Query: 363 LTKPVVKAFRYDNNGDIIDSEIIDLSDPAASDSILQRGGEEWLK 406 LT+PV+K + ++DL +D+I+ RG EEW + Sbjct: 344 LTRPVLKVLHLQGPHETTIGGVLDLMAWPRTDTIIGRGDEEWTR 387 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 324 bits (831), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 170/394 (43%), Positives = 241/394 (61%), Gaps = 13/394 (3%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDVAHLKQARGSGIMYCWIDVEQGK 62 +LK I +D+L LGMY W+ HPF + F ++ + L+ R I I+ +G+ Sbjct: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 Query: 63 DVAVEEPEQQEAEPEAGPESGAEPVSAEAAPGLRDELPVAIGADPFFEELEQAQRLCENA 122 DV EE E E + + + +A P R + + E E+AQR+ ++ Sbjct: 61 DVTREELE--EDDADQDAPAAPPADAANRNPASRTDTAI---------ERERAQRIISSS 109 Query: 123 KDTTKAMFADIRMGRVIDMDRCVDLVDQVTASIMRNSAAMTSIVRLKLADDYTYMHSVAV 182 K T MF++ RMG+ ID+ +LV ++TAS+ RN+ A+ S+ RLK DDYTY+HSVAV Sbjct: 110 KSTVLHMFSEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAV 169 Query: 183 CTLMVALGRTIGMDEPACKEAGLAGLLHDVGKAFIPVPILHKAGGLTPAELDIVRRHPVL 242 C +M++L +GM K+AG+AGLLHDVGK +P+ IL+K LT E VR H V Sbjct: 170 CAMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQ 229 Query: 243 GYNHLIKNPNIPQYAIEVCLHHHEKIDGTGYPDNLSGAGISLLSRMASICDVYDAVTSDR 302 G+ L + I + A++V LHHHEKIDG+GYP NL IS++++M ++CDVYDA+TS+R Sbjct: 230 GHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNR 289 Query: 303 PYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQSAGG 362 PYK GWDPA S+ MA+ +GHFD + AFVK +GIYP GS + ++SGRLG+V+ Q Sbjct: 290 PYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQ 349 Query: 363 LTKPVVKAFRYDNNGDIIDSEIIDLSDPAASDSI 396 L P VK F N ++SEIIDL+ +SD I Sbjct: 350 LLTPRVKVFYSTANRMPLESEIIDLAH--SSDKI 381 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 319 bits (817), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 167/390 (42%), Positives = 229/390 (58%), Gaps = 10/390 (2%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDVAHLKQARGSGIMYCWIDVEQGK 62 +LK I +L +GMY F AW P W+ FLL+ L + S I ID+ +G Sbjct: 1 MLKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGA 60 Query: 63 DVAVEEPEQQEA-EPEAGPESGAEPVSAEAAPGLRDELPVAIGADPFFEELEQAQRLCEN 121 DV +E EQ E G + E A G R ++ E E+A R+ + Sbjct: 61 DVQTDEAEQGNGVESSLGKNAEIEVAQALPEAGKRTDIQA---------ERERAARIISS 111 Query: 122 AKDTTKAMFADIRMGRVIDMDRCVDLVDQVTASIMRNSAAMTSIVRLKLADDYTYMHSVA 181 ++ T MF+ RMG+ ID+ DLV ++ AS+ RN+ AM S+VRLK AD+YTYMHSVA Sbjct: 112 SEKTVLTMFSQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVA 171 Query: 182 VCTLMVALGRTIGMDEPACKEAGLAGLLHDVGKAFIPVPILHKAGGLTPAELDIVRRHPV 241 VC +M++L +G+ ++AG+AGLLHD+GK +P IL+K LT E V+ HP+ Sbjct: 172 VCAMMISLADQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPL 231 Query: 242 LGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGYPDNLSGAGISLLSRMASICDVYDAVTSD 301 G+ L + P I ++V LHHHEK+DGTGYP LSG IS+ +RM +ICDVYDA+TSD Sbjct: 232 AGHRLLQELPGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSD 291 Query: 302 RPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQSAG 361 RPYK GWDP+ S+ MA GHFD +L AFVK +GIYP+GS + M++G L +V+ Q Sbjct: 292 RPYKSGWDPSRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPD 351 Query: 362 GLTKPVVKAFRYDNNGDIIDSEIIDLSDPA 391 L P+V+ F N + +IDL A Sbjct: 352 SLLAPLVRIFYSTNKKIAVTPTLIDLRRSA 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,297,391 Number of extensions: 52107 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 10 Length of query: 423 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 335 Effective length of database: 1,203,190 Effective search space: 403068650 Effective search space used: 403068650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)