BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0136.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 263 1e-71 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 256 1e-69 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 225 3e-60 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 179 1e-46 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 173 8e-45 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 263 bits (671), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 115/202 (56%), Positives = 157/202 (77%) Query: 7 KAFIPVPILHKAGGLTPAELDIVRRHPVLGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGY 66 KAFIP +L+K G L+ E +++HP LGY +L ++PN+P YA +VCLHHHEKIDG GY Sbjct: 198 KAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGY 257 Query: 67 PDNLSGAGISLLSRMASICDVYDAVTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFV 126 P+ L I+L SRMA+ICDVYDA+TSDRPYK+ WDPAE+++AMASW+GHFD+ + AFV Sbjct: 258 PEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTGHFDRAMFLAFV 317 Query: 127 KTLGIYPIGSLLKMESGRLGLVIRQSAGGLTKPVVKAFRYDNNGDIIDSEIIDLSDPAAS 186 KTLGIYP+GS++K+ESGR G VIRQ+A LT+PV+K F YD++ + D+EI+DL + Sbjct: 318 KTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDTEILDLLANPEA 377 Query: 187 DSILQRGGEEWLKYNGLNKLWM 208 D+I+ RGGEEW + + L+ + + Sbjct: 378 DTIVARGGEEWARLSHLDAMQL 399 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 256 bits (653), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 118/197 (59%), Positives = 151/197 (76%) Query: 7 KAFIPVPILHKAGGLTPAELDIVRRHPVLGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGY 66 KAFIP+ IL+K L+ E I++RHP LGY++L +P Y ++VCLHHHEKIDG+GY Sbjct: 192 KAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGY 251 Query: 67 PDNLSGAGISLLSRMASICDVYDAVTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFV 126 P G IS+ SRMA++CDVYDAVTS+RPYK GWDPAESL+ MASW GHFD+ + AFV Sbjct: 252 PSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQGHFDRPIFLAFV 311 Query: 127 KTLGIYPIGSLLKMESGRLGLVIRQSAGGLTKPVVKAFRYDNNGDIIDSEIIDLSDPAAS 186 K LGIYPIGS++K++SGR LVIRQ+ LTKPVVKAF + ++++SEIIDLS P + Sbjct: 312 KALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVESEIIDLSAPGQT 371 Query: 187 DSILQRGGEEWLKYNGL 203 D+I +R G++WLK NGL Sbjct: 372 DAIAERSGQDWLKSNGL 388 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 225 bits (573), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 102/193 (52%), Positives = 138/193 (71%) Query: 7 KAFIPVPILHKAGGLTPAELDIVRRHPVLGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGY 66 KAFI IL+K G LT E +++RHP LGY +LI+ P++P YA +VC HHHE++DG GY Sbjct: 195 KAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGY 254 Query: 67 PDNLSGAGISLLSRMASICDVYDAVTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFV 126 PD L I+L +RM ++CDVYDA+TS+RPYK GWDPAE+++ MA W GHFD+ LL AFV Sbjct: 255 PDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMARWEGHFDRRLLLAFV 314 Query: 127 KTLGIYPIGSLLKMESGRLGLVIRQSAGGLTKPVVKAFRYDNNGDIIDSEIIDLSDPAAS 186 KTLGIYP+GSL+++ESG G+VIRQ+ LT+PV+K + ++DL + Sbjct: 315 KTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGPHETTIGGVLDLMAWPRT 374 Query: 187 DSILQRGGEEWLK 199 D+I+ RG EEW + Sbjct: 375 DTIIGRGDEEWTR 387 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 179 bits (454), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 87/175 (49%), Positives = 117/175 (66%) Query: 7 KAFIPVPILHKAGGLTPAELDIVRRHPVLGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGY 66 K +P+ IL+K LT E VR H V G+ L + I + A++V LHHHEKIDG+GY Sbjct: 201 KMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGY 260 Query: 67 PDNLSGAGISLLSRMASICDVYDAVTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFV 126 P NL IS++++M ++CDVYDA+TS+RPYK GWDPA S+ MA+ +GHFD + AFV Sbjct: 261 PFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFV 320 Query: 127 KTLGIYPIGSLLKMESGRLGLVIRQSAGGLTKPVVKAFRYDNNGDIIDSEIIDLS 181 K +GIYP GS + ++SGRLG+V+ Q L P VK F N ++SEIIDL+ Sbjct: 321 KAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYSTANRMPLESEIIDLA 375 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 173 bits (439), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 85/178 (47%), Positives = 112/178 (62%) Query: 7 KAFIPVPILHKAGGLTPAELDIVRRHPVLGYNHLIKNPNIPQYAIEVCLHHHEKIDGTGY 66 K +P IL+K LT E V+ HP+ G+ L + P I ++V LHHHEK+DGTGY Sbjct: 204 KMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQELPGIGDAPLDVSLHHHEKMDGTGY 263 Query: 67 PDNLSGAGISLLSRMASICDVYDAVTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFV 126 P LSG IS+ +RM +ICDVYDA+TSDRPYK GWDP+ S+ MA GHFD +L AFV Sbjct: 264 PYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDPSRSIQHMAQSKGHFDFDVLQAFV 323 Query: 127 KTLGIYPIGSLLKMESGRLGLVIRQSAGGLTKPVVKAFRYDNNGDIIDSEIIDLSDPA 184 K +GIYP+GS + M++G L +V+ Q L P+V+ F N + +IDL A Sbjct: 324 KAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLVRIFYSTNKKIAVTPTLIDLRRSA 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 712,767 Number of extensions: 28183 Number of successful extensions: 73 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 70 Number of HSP's successfully gapped: 9 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)