BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0135.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310562|ref|YP_003774654.1| composite two-component transcr... 144 2e-35 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 79 6e-16 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 75 8e-15 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 72 9e-14 gi|300313070|ref|YP_003777162.1| two component sensor histidine ... 62 1e-10 >gi|300310562|ref|YP_003774654.1| composite two-component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 604 Score = 144 bits (362), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 102/263 (38%), Positives = 147/263 (55%), Gaps = 15/263 (5%) Query: 100 SPENTIVTVYICIVLAGMTGGSVASLSSFWPAYYVYALPTVLPFAMRSFAHGTHLFSVLG 159 SP +I+ + VLAG+ G S++ LS P + + + + A + VL Sbjct: 121 SPGGSILAIG---VLAGVAGNSMSILSPVLPVFISFCIVELGMLAGWVWIMHDPSLQVLS 177 Query: 160 VLSLFLLGVNLAYSRVIQRTVRDAIALRFENNALIRQLTEEKER-------AEQANRSKS 212 +L + L +R R + AI LR+EN LIR+L E ER AEQAN +KS Sbjct: 178 AAALLYVASLLVQARNSARAAQAAITLRYENTELIRRLQHESERTQQALAQAEQANAAKS 237 Query: 213 QFLAAASHDLRQPVHALGLYIATLRTVAQAPHIEPGTVDMLAGRLQSALTGMSQLLNVLL 272 +FLAAASHDLRQP+HA GL++ L T ++P D++A +L+ A + +L+ LL Sbjct: 238 KFLAAASHDLRQPIHAQGLFLEVLNTTP----LDPMQRDVVA-KLRRAADNCADMLHTLL 292 Query: 273 DISRLDAGVVRIEKHRFSVQETFTLLANQFAQAAAAKGLQLKVRPTTAWLDTDPVLLHNI 332 D SR++AGVV + RF VQ ++ + A A AKGL + R + A + D L I Sbjct: 293 DYSRIEAGVVEPQVTRFRVQALVGRISQEMAAQAEAKGLVFRCRESRAVVSADASLTELI 352 Query: 333 LANLLSNAVRYTENGKILLACRR 355 L NL+SNA+RYT+ G IL+ACR+ Sbjct: 353 LRNLVSNAIRYTDRGGILVACRQ 375 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 79.3 bits (194), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 51/162 (31%), Positives = 85/162 (52%), Gaps = 7/162 (4%) Query: 196 QLTEEKERAEQANRSKSQFLAAASHDLRQPVHALGLYIATLRTVAQAPHIEPGTVDMLAG 255 +L KE AE AN +KS+F+A SH+LR P++++ Y L A P P D + G Sbjct: 673 ELQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIP---PARKDAI-G 728 Query: 256 RLQSALTGMSQLLNVLLDISRLDAGVVRIEKHRFSVQETFTLLANQFAQAAAAKGLQLKV 315 ++ + + L++ LLDI++++AG +R+ +++ + FA A KGL + Sbjct: 729 VIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQKGLHFRF 788 Query: 316 RPTT---AWLDTDPVLLHNILANLLSNAVRYTENGKILLACR 354 + + D L IL NLL NAV++T++G + L R Sbjct: 789 DKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLR 830 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 75.5 bits (184), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 55/158 (34%), Positives = 86/158 (54%), Gaps = 5/158 (3%) Query: 197 LTEEKERAEQANRSKSQFLAAASHDLRQPVHALGLYIATLRTVAQAPHIEPGTVDMLAGR 256 L E+ E+AN+ KS+FLA SH+LR P+++ L +A L + ++ V A Sbjct: 461 LVEKATELERANQYKSEFLANMSHELRTPLNST-LILAKLLADNKQGNLSDEQV-RFAQT 518 Query: 257 LQSALTGMSQLLNVLLDISRLDAGVVRIEKHRFSVQETFTLLANQFAQAAAAKGLQLKVR 316 + SA + L+N +LD+S+++AG + I F + + L+ FA A KGL KV Sbjct: 519 ISSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVE 578 Query: 317 PTTA---WLDTDPVLLHNILANLLSNAVRYTENGKILL 351 ++TD L IL NLLSNA+++TE G++ L Sbjct: 579 VEPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSL 616 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 72.0 bits (175), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 49/170 (28%), Positives = 87/170 (51%), Gaps = 12/170 (7%) Query: 187 RFENNALIRQLTEEKERAEQANRSKSQFLAAASHDLRQPVHA-LGLYIATLRTVAQAPHI 245 R E +AL+ T KE A+ AN +KS FLA SH++R P++ LGL + + + Sbjct: 326 RREEDALL--ATRLKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQR 383 Query: 246 EPGTVDMLAGRLQSALTGMSQLLNVLLDISRLDAGVVRIEKHRFSVQETFTLLANQFAQA 305 + A + A G+ +++N +LD S++++G + +E F L++ + + Sbjct: 384 D------YADSIHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPS 437 Query: 306 AAAKGLQLKVR---PTTAWLDTDPVLLHNILANLLSNAVRYTENGKILLA 352 K +Q + P + DP L +L NLLSNA+++T+ G + L+ Sbjct: 438 LQRKDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLS 487 >gi|300313070|ref|YP_003777162.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 939 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 42/142 (29%), Positives = 73/142 (51%), Gaps = 4/142 (2%) Query: 206 QANRSKSQFLAAASHDLRQPVHALGLYIATLRTVAQAPHIEPGTVDMLAGRLQSALTGMS 265 ++ R ++ L+A SHDLR P+ +L + + +A ++ M A + M Sbjct: 703 ESERLRNSLLSAISHDLRTPLTSL---VGSADVLAANARLDDDARAM-ARTISEQSQKMV 758 Query: 266 QLLNVLLDISRLDAGVVRIEKHRFSVQETFTLLANQFAQAAAAKGLQLKVRPTTAWLDTD 325 L+N LLD++RL AGVV + + +++E A+A + + ++ P L D Sbjct: 759 GLMNNLLDMARLQAGVVTLNRQWNALEELAGSALRLLAKALEGRQVDTRIAPELPLLRVD 818 Query: 326 PVLLHNILANLLSNAVRYTENG 347 VLL +LANL+ NA++Y+ G Sbjct: 819 AVLLERVLANLVENAIKYSPPG 840 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.133 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 986,694 Number of extensions: 32181 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 8 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)