BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0127.p (221 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 181 5e-47 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 152 3e-38 gi|300313041|ref|YP_003777133.1| major facilitator superfamily p... 140 1e-34 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 134 6e-33 gi|300311780|ref|YP_003775872.1| major facilitator superfamily p... 129 1e-31 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 181 bits (458), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 96/211 (45%), Positives = 129/211 (61%), Gaps = 2/211 (0%) Query: 1 LIFNKLFFPAFDPLTGTLASFATYTVGFLARPLGGIVFGHFGDKIGRKSMLLITLLMMGI 60 LIF +LFFP DP T +AS ATY VG++ARP+G V GH GD GRK +LL + +MG Sbjct: 40 LIFPQLFFPKGDPTTAIIASLATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGF 99 Query: 61 PTIIIGLIPTYEQIGYWAAVILVIMRIIQGLAVGGEWGGAVLMAVEHAPKGKKGFFGSLP 120 TI +GL+PTY+Q+G WA +LVIMR+ QG AV GE GA M +EH+P G++G+F S Sbjct: 100 STIGVGLLPTYDQVGLWAPALLVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFT 159 Query: 121 QTGVAPGLILSSLAMAAVARLPEADMLG-WAWRIPFVASVALLIVGWFIRIKVAESPDFE 179 GV G IL++ +A A+ W WRIPF+ S ++I GW IR V E+P F+ Sbjct: 160 LQGVQAGQILAAAVFLPLAHYMPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFK 219 Query: 180 NMKKHGQEVKLPVVYVLKHHWKSLL-TVCGA 209 K + + PVV + ++L VC A Sbjct: 220 AADKEELKRRSPVVDAFRDCSPNMLRVVCMA 250 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 152 bits (383), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 88/203 (43%), Positives = 121/203 (59%), Gaps = 2/203 (0%) Query: 2 IFNKLFFPAFDPLTGTLASFATYTVGFLARPLGGIVFGHFGDKIGRKSMLLITLLMMGIP 61 I K FF DP T + + + GF+ RP G +VFG GD +GRK L+T+L+MG Sbjct: 44 IIAKQFFAGTDPNTAFIFALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGAS 103 Query: 62 TIIIGLIPTYEQIGYWAAVILVIMRIIQGLAVGGEWGGAVLMAVEHAPKGKKGFFGSLPQ 121 T I+G++P Y IG A +IL+I+RI+QGLA+GGE+GGA EHAP+G++G F + Q Sbjct: 104 TFIVGVLPNYNSIGITAPIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQ 163 Query: 122 TGVAPGLILSSLAMAAV-ARLPEADMLGWAWRIPFVASVALLIVGWFIRIKVAESPDFEN 180 T GL LS L + V + EA W WRIPF+ S+ LL + +IR+ + ESP F Sbjct: 164 TTATMGLFLSLLVILGVRTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLK 223 Query: 181 MKKHGQEVKLPVVYVLKHHWKSL 203 MK G+ K P+ WK+L Sbjct: 224 MKAEGKTSKAPLTEAF-GQWKNL 245 >gi|300313041|ref|YP_003777133.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 436 Score = 140 bits (352), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 80/202 (39%), Positives = 121/202 (59%), Gaps = 5/202 (2%) Query: 2 IFNKLFFPAFDP-LTGTLASFATYTVGFLARPLGGIVFGHFGDKIGRKSMLLITLLMMGI 60 + KLFFPA P L +F + G+LARP+GGI+ H+GD IGRK M +++ +M + Sbjct: 50 VVGKLFFPADMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAV 109 Query: 61 PTIIIGLIPTYEQIGYWAAVILVIMRIIQGLAVGGEWGGAVLMAVEHAPKGKKGF-FGSL 119 PT++IGL+PTY IG A ++L++MR +QG A+GGE GA + EH P + F GSL Sbjct: 110 PTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSL 169 Query: 120 PQTGVAPGLILSSLAMAAVARLPE-ADMLGWAWRIPFVASVALLIVGWFIRIKVAESPDF 178 G+ G++L SL + E A++ G+AWRIPF+ +V ++R + E+P F Sbjct: 170 -TAGITGGILLGSLMGIFINSAFEPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVF 228 Query: 179 ENMKKHGQEV-KLPVVYVLKHH 199 + +E +LP+ VL+ H Sbjct: 229 RELAARRKEANELPLKTVLREH 250 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 134 bits (337), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 73/191 (38%), Positives = 117/191 (61%), Gaps = 2/191 (1%) Query: 1 LIFNKLFFPAFDPLTGTLASFATYTVGFLARPLGGIVFGHFGDKIGRKSMLLITLLMMGI 60 +I KLFFP + LT L + AT+ VGF RP+GGI+ G + D++GRK+ L +T+L+M + Sbjct: 45 VIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAV 104 Query: 61 PTIIIGLIPTYEQIGYWAAVILVIMRIIQGLAVGGEWGGAVLMAVEHAPKGKKGFFGSLP 120 T +IGL PTYE IG +A +I+V+ R++QG + GGE G A E+AP+ ++ ++ S Sbjct: 105 GTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWI 164 Query: 121 QTGVAPGLIL-SSLAMAAVARLPEADMLGWAWRIPFVASVALLIVGWFIRIKVAESPDF- 178 Q + ++L +++ A L A + W WR+PF+ + + VG++IR + E+P F Sbjct: 165 QASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFL 224 Query: 179 ENMKKHGQEVK 189 E K +K Sbjct: 225 EEKDKTDSPLK 235 >gi|300311780|ref|YP_003775872.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 129 bits (325), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 67/182 (36%), Positives = 106/182 (58%), Gaps = 1/182 (0%) Query: 1 LIFNKLFFPAFDPLTGTLASFATYTVGFLARPLGGIVFGHFGDKIGRKSMLLITLLMMGI 60 L+F +FFP L GTL +F + FLARP+G +F GR+ L + L M+G Sbjct: 60 LVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGF 119 Query: 61 PTIIIGLIPTYEQIGYWAAVILVIMRIIQGLAVGGEWGGAVLMAVEHAPKGKKGFFGSLP 120 T I L+PT+E IG WA ++L ++R+ QGLA+GG W G + +AP+ K+G++ +L Sbjct: 120 STAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAALG 179 Query: 121 QTGVAPGLIL-SSLAMAAVARLPEADMLGWAWRIPFVASVALLIVGWFIRIKVAESPDFE 179 Q G G ++ +SL ++ L AD L W WR PF + A+ +V F R+++ +P++ Sbjct: 180 QLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYA 239 Query: 180 NM 181 + Sbjct: 240 KL 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.144 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 683,834 Number of extensions: 26245 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 12 Length of query: 221 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 139 Effective length of database: 1,231,600 Effective search space: 171192400 Effective search space used: 171192400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)