BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0087.p (605 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313709|ref|YP_003777801.1| L-glutamine:D-fructose-6-phosph... 1116 0.0 gi|300313838|ref|YP_003777930.1| glucosamine 6-phosphate synthas... 113 3e-26 gi|300312583|ref|YP_003776675.1| glutamine phosphoribosylpyropho... 71 2e-13 >gi|300313709|ref|YP_003777801.1| L-glutamine:D-fructose-6-phosphate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 605 Score = 1116 bits (2886), Expect = 0.0, Method: Compositional matrix adjust. Identities = 547/605 (90%), Positives = 584/605 (96%) Query: 1 MCGIVGAVAQKDVTPILLEGLKRLEYRGYDSCGVAVHMDGKLQRSRSTARVAELQKQVAE 60 MCGIVGAVAQ++VTPILLEGLKRLEYRGYDSCGVA+H+DGKLQRSRST+RVAELQ+Q+A+ Sbjct: 1 MCGIVGAVAQQNVTPILLEGLKRLEYRGYDSCGVALHVDGKLQRSRSTSRVAELQQQIAQ 60 Query: 61 THLAGFTGIAHTRWATHGAPATHNAHPHFSRDRIALVHNGIIENYEELRKELQGVGYVFE 120 + L+GFTGIAHTRWATHGAPATHNAHPHFSRDRIALVHNGIIEN++ELR ELQ VGYVFE Sbjct: 61 SGLSGFTGIAHTRWATHGAPATHNAHPHFSRDRIALVHNGIIENHDELRAELQAVGYVFE 120 Query: 121 SQTDTEVIAHLVDHMYTGDLFETVQQAVRRLAGAYAIAVFCVDEPHRVVAARQGSPLIVG 180 SQTDTEVIAHLVDHMY GDLF TVQQAVRRL GAYAIAVFC DEPHRVVAARQGSPLIVG Sbjct: 121 SQTDTEVIAHLVDHMYDGDLFNTVQQAVRRLTGAYAIAVFCADEPHRVVAARQGSPLIVG 180 Query: 181 VGEGQNFVASDAMALAGTTDQIIYLEEGDVVDLQLQRYWIVDAAGKSVTREVKTVQAFTS 240 VGEGQNFVASDAMALAGTTDQIIYLEEGDVVDLQLQ+ WIVDAAG+ REV+TVQA+TS Sbjct: 181 VGEGQNFVASDAMALAGTTDQIIYLEEGDVVDLQLQKVWIVDAAGRPAEREVRTVQAYTS 240 Query: 241 AVELGPYQHYMQKEIFEQPRAIADTLEGVSGIMPELFGDGAHKVFKGVDSVLILACGTSY 300 AVELGPYQHYMQKEIFEQPRAIADTLEGV GIMPELFGD A+KVFK +DS+LILACGTSY Sbjct: 241 AVELGPYQHYMQKEIFEQPRAIADTLEGVVGIMPELFGDKAYKVFKEIDSILILACGTSY 300 Query: 301 YSGLTAKYWLESIARIPVNVEIASEYRYRDSVPNPNSLVVTISQSGETADTLAALKHARA 360 Y+GLTAKYWLESIA+IPVNVEIASEYRYRDSVPNP SLVVTISQSGETADTLAALKHAR+ Sbjct: 301 YAGLTAKYWLESIAKIPVNVEIASEYRYRDSVPNPKSLVVTISQSGETADTLAALKHARS 360 Query: 361 QGMQHTLTICNVATSAMVRESQLAYITRAGVEVGVASTKAFTTQLAALFLLTLALAQTRG 420 GMQHTLTICNVATSAMVRE +LAYITRAGVEVGVASTKAFTTQLAALFLLTLALAQTRG Sbjct: 361 LGMQHTLTICNVATSAMVRECELAYITRAGVEVGVASTKAFTTQLAALFLLTLALAQTRG 420 Query: 421 LLSDEQEAEHLKAMRHLPAALQSVLALEPSIAAWAEAFARKENALFLGRGLHYPIALEGA 480 LL++EQEAEHLKAMRHLPAALQSVLALEPSI AWAE+FAR+ENALFLGRGLHYPIALEGA Sbjct: 421 LLTEEQEAEHLKAMRHLPAALQSVLALEPSIVAWAESFARRENALFLGRGLHYPIALEGA 480 Query: 481 LKLKEITYIHAEAYAAGELKHGPLALVTEEMPVVTVAPNDALIEKLKSNMQEVRARGGQL 540 LKLKEITYIHAEAYAAGELKHGPLALVT++MPVVT+APNDAL+EKLKSNMQEVRARGGQL Sbjct: 481 LKLKEITYIHAEAYAAGELKHGPLALVTDQMPVVTIAPNDALVEKLKSNMQEVRARGGQL 540 Query: 541 YVFADAGSSIASSEGVHVIRLPEHYGLLSPILHVVPLQLLAYHTALARGTDVDKPRNLAK 600 YVFAD S I+S+EG+HVIR+PEHYG+LSPILHVVPLQLL+YHTALARGTDVDKPRNLAK Sbjct: 541 YVFADGDSHISSAEGLHVIRMPEHYGVLSPILHVVPLQLLSYHTALARGTDVDKPRNLAK 600 Query: 601 SVTVE 605 SVTVE Sbjct: 601 SVTVE 605 >gi|300313838|ref|YP_003777930.1| glucosamine 6-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 88/268 (32%), Positives = 142/268 (52%), Gaps = 23/268 (8%) Query: 336 NSLVVTISQSGETADTLAALKHARAQGMQHTLTICNVATSAMVRESQLAYITRAGVEVGV 395 ++L + +SQSG++ D + +++ AQG T+ + N A S + +Q + AGVE V Sbjct: 84 DALAIAVSQSGQSPDVVEPIRYFHAQGAT-TVALVNQADSPLASAAQWSLPLHAGVESSV 142 Query: 396 ASTKAFTTQLAALFLLTLALAQTRGLLSDEQEAEHLKAMRHLPAALQSVLALEPSIAAWA 455 A+TK+F L A LLT + +A L A+ LP AL++ A + S A Sbjct: 143 AATKSFIASLVASALLTAHW---------QDDAALLNALAQLPDALRAACACDWSDAV-- 191 Query: 456 EAFARKENALFLGRGLHYPIALEGALKLKEITYIHAEAYAAGELKHGPLALVTEEMPVVT 515 +A + + +GRG+ +P+ALE ALK KE I AEA++ E+KHGP+AL+ E P++ Sbjct: 192 QALTPADRIMVVGRGIGFPLALEAALKCKETCAIQAEAFSGAEIKHGPIALIEEGYPLLM 251 Query: 516 VA---PNDALIEKLKSNMQEVRARGGQLYVFADAGSSIASSEGVHVIRLPEHYGLLSPIL 572 A P+ A + L + E+R RG ++ + A + + V P+ L PI+ Sbjct: 252 FAIRGPSQAGMLALAA---EMRVRGAKV-LLAAPEDVVERDLTLPVAATPD----LDPIV 303 Query: 573 HVVPLQLLAYHTALARGTDVDKPRNLAK 600 + ++A + ARG D D+PR+L K Sbjct: 304 AIQAFYVMAAQLSAARGMDADRPRHLNK 331 >gi|300312583|ref|YP_003776675.1| glutamine phosphoribosylpyrophosphate amidotransferase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 71.2 bits (173), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 60/222 (27%), Positives = 102/222 (45%), Gaps = 31/222 (13%) Query: 1 MCGIVGAVAQKDVTPILLEGLKRLEYRGYDSCGVAVHMDGKLQRSRSTARVAELQKQVAE 60 MCGIVG V+ V +L + L L++RG D+ G+A + ++ V ++ + Sbjct: 1 MCGIVGIVSHSPVNQMLYDALLLLQHRGQDAAGIATNHSNGFSMHKANGLVRDVFRTRNM 60 Query: 61 THLAGFTGIAHTRWATHGAPAT-HNAHPHFSRDR--IALVHNGIIENYEELRKEL-QGVG 116 L G TGI R+ T G+ ++ A P + I L HNG + N E+L+ E+ + Sbjct: 61 RSLPGNTGIGQVRYPTAGSSSSEEEAQPFYVNAPFGIILAHNGNLTNAEQLKIEMFKNDR 120 Query: 117 YVFESQTDTE----VIAHLVDHMYTG------DLFETVQQAVRRLAGAYAI-------AV 159 + +DTE V+AH + TG LF+ V +R+ G+YA+ + Sbjct: 121 RHINTDSDTEVLLNVLAHEIQQATTGYSLDPAALFKAVAMVHKRVRGSYAVVAQIAGYGL 180 Query: 160 FCVDEPHRVVAARQGSPLIVGVGEGQN----FVASDAMALAG 197 +P+ + P+ +G E +AS+++AL G Sbjct: 181 LGFRDPYGI------RPMCLGFNESDKGVEYMMASESVALEG 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,838,113 Number of extensions: 77953 Number of successful extensions: 281 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 3 Length of query: 605 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 514 Effective length of database: 1,188,985 Effective search space: 611138290 Effective search space used: 611138290 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)