BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0052.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 540 e-155 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 404 e-114 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 89 6e-19 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 83 3e-17 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 76 4e-15 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 540 bits (1392), Expect = e-155, Method: Compositional matrix adjust. Identities = 257/323 (79%), Positives = 286/323 (88%), Gaps = 5/323 (1%) Query: 1 VELLKAGHQVVVFDNFCNSFPEAPRRVEKEXKKKITGKTLTLVKGDIRDRAAIEQALREH 60 VELLKAGH+V+VFDNFCNS PE+ +RV++ ITGKTL+LVKGDIR+R +E LRE Sbjct: 18 VELLKAGHEVIVFDNFCNSSPESVKRVQQ-----ITGKTLSLVKGDIRNREQLESVLREF 72 Query: 61 ACDAVIHFAGLKAVGESVVKPLEYYDNNVVGTVRLLEAMQAANVKTLVFSSSATVYGNPQ 120 C+AVIHFAGLKAVGESV +PL YYDNNVVGTVRLLEAMQA VKT+VFSSSATVYG+PQ Sbjct: 73 KCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIVFSSSATVYGDPQ 132 Query: 121 YLPLTESHPLSASNPYGRSKIVIEDMLRDFHIAHPDWCIALLRYFNPVGAHESGLIGEDP 180 YLPL E+HPLSASNPYGRSKIVIEDMLRD++ AHPDW IALLRYFNPVGAHESGLIGEDP Sbjct: 133 YLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPVGAHESGLIGEDP 192 Query: 181 QGIPNNLMPFVAQVAIGKRDKLNIWGGDYDTPDGTGVRDFIHVVDLAVGHLNALEYLKKP 240 GIPNNLMPFVAQVAIG+R++LNIWGGDY TPDGTGVRDFIHVVDLA GH+ AL+ L P Sbjct: 193 GGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLARGHVAALQALDAP 252 Query: 241 QCFAVNLGTGIGYSVLDVVKAFEKACGKTLPFEIAARRPGDIAACYADTALAKSTLGWEA 300 QCFAVNLGTG+GYSVLDV+KA+EKACGKTLP+ IA RRPGDIA+CYA+ A AK LGWEA Sbjct: 253 QCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYANPAEAKRKLGWEA 312 Query: 301 TRSLETMCADSWRWQQGNPNGFR 323 T LE MCADSW+WQQ NPNGF+ Sbjct: 313 TLGLEAMCADSWKWQQMNPNGFK 335 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 404 bits (1037), Expect = e-114, Method: Compositional matrix adjust. Identities = 193/316 (61%), Positives = 237/316 (75%), Gaps = 6/316 (1%) Query: 1 VELLKAGHQVVVFDNFCNSFPEAPRRVEKEXKKKITGKTLTLVKGDIRDRAAIEQALREH 60 VEL+ AG+ VVV DN CNS R+ +I+G+ V+GDIRDRA ++ + H Sbjct: 21 VELIHAGYAVVVLDNLCNSRASVIDRI-----AQISGQRPHFVQGDIRDRAVLDGIFKSH 75 Query: 61 ACDAVIHFAGLKAVGESVVKPLEYYDNNVVGTVRLLEAMQAANVKTLVFSSSATVYGNPQ 120 +AVIHFAGLKAVGESV +PL YYDNNV G+ L EAM A VK +VFSSSATVYG+P Sbjct: 76 RIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNIVFSSSATVYGDPA 135 Query: 121 YLPLTESHPLSASNPYGRSKIVIEDMLRDFHIAHPDWCIALLRYFNPVGAHESGLIGEDP 180 +P+TE PLSA+NPYGRSK+++E +L D H+A P W IALLRYFNPVGAHESGLIGEDP Sbjct: 136 SVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNPVGAHESGLIGEDP 195 Query: 181 QGIPNNLMPFVAQVAIGKRDKLNIWGGDYDTPDGTGVRDFIHVVDLAVGHLNALEYLKK- 239 GIPNNL+PF+AQVA G+R L+++G DY TPDGTGVRD+IHVVDLA+GHL L L++ Sbjct: 196 SGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVDLALGHLKTLRKLEQG 255 Query: 240 PQCFAVNLGTGIGYSVLDVVKAFEKACGKTLPFEIAARRPGDIAACYADTALAKSTLGWE 299 P + NLGTG G SVL+++ AFE+ACG LP+++ RRPGDIA CYA T A+ LGW Sbjct: 256 PGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIACCYAATERAERELGWR 315 Query: 300 ATRSLETMCADSWRWQ 315 A R +E MCAD+WRWQ Sbjct: 316 AQRGIEAMCADTWRWQ 331 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 91/316 (28%), Positives = 137/316 (43%), Gaps = 41/316 (12%) Query: 9 QVVVFDNFCNSFPEAPRRVEKEXKKKITGKTLTLVKGDIRDRAAIEQALREHACDAVIHF 68 +++++DNF E + K+ + KI V GDI ++ AL D V H Sbjct: 32 EILIYDNFLRGSRENLQNALKDPRVKIYD-----VGGDIMQTDILQSAL--EGADGVFHL 84 Query: 69 AGLKAVGESVVKPLEYYDNNVVGTVRLLEAMQAANVKTLVFSSSATVYGNPQYLPLTESH 128 A L + + P +D NV GT ++EA A VK LV+SSSA+VYG+ P+ E H Sbjct: 85 AALWLL-QCHEYPRSAFDVNVRGTFNVMEACVAKGVKRLVYSSSASVYGDAVREPMDEDH 143 Query: 129 PLSASNPYGRSKIVIEDMLRDFHIAHPDWCIALLRYFNPVGAHESGLIGEDPQGIPNNLM 188 P + N YG +KI E MLR FH + + LRY N G +D G Sbjct: 144 PYNNKNFYGATKIAGETMLRSFHHRYGLNYVG-LRYMNVYGPR------QDYHG------ 190 Query: 189 PFVAQV-----AIGKRDKLNIWGGDYDTPDGTGVRDFIHVVDLAVGHLNALEYLKKPQCF 243 ++A + AI K + I G DG+ DF+ V D + ++ A++ Q + Sbjct: 191 AYIAVIMKMLDAIDKGESPTIMG------DGSEAFDFVAVEDCGLANVCAMKAETADQYY 244 Query: 244 AVNLGTGIGYSVLDVVKAFEKACGKTLPFEIAARRPGDIA----ACYADTALAKSTLGWE 299 N+GTG S+ ++ + + G P A R + C A S +G+ Sbjct: 245 --NVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLVRNRIGC---PKKASSEIGFT 299 Query: 300 ATRSLETMCADSWRWQ 315 AT L W+ Sbjct: 300 ATIDLREGLKRLIEWR 315 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 90/327 (27%), Positives = 139/327 (42%), Gaps = 33/327 (10%) Query: 3 LLKAGHQVVVFDNFCNSFPEAPRRVEKEXKKKITGK---TLTLVKGDIRD----RAAIE- 54 LLK +VV DNF F E K +T + V+GDIR RAA+ Sbjct: 36 LLKLNQRVVGLDNFSTGFQHNL----DEVKALVTPEQWANFRFVEGDIRKLDDCRAAMHH 91 Query: 55 QALREHACDAVIHFAGLKAVGESVVKPLEYYDNNVVGTVRLLEAMQAANVKTLVFSSSAT 114 +AL + + V+H A L +V S+ P+ NN+ G + +L A + A VK V+++S++ Sbjct: 92 EALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVAARDARVKRFVYAASSS 151 Query: 115 VYGNPQYLPLTESHPLSASNPYGRSKIVIEDMLRDFHIAHPDWCIALLRYFNPVGAHESG 174 YG+ LP E +PY +K V E F + I LRYFN G Sbjct: 152 TYGDHPGLPKVEDRIGKPLSPYAVTKFVNELYADVFARTYGLQTIG-LRYFNIFGRR--- 207 Query: 175 LIGEDPQGIPNNLMPFVAQVAIGKRDKLNIWGGDYDTPDGTGVRDFIHVVDLAVGHLNAL 234 ++P G ++P AI K +++ I G DG RDF +V + +L A Sbjct: 208 ---QNPDGAYAAVIPKWIAAAI-KGEQIYING------DGETSRDFCYVDNAVQANLLAA 257 Query: 235 EYLKKPQCFAV-NLGTGIGYSVLDVVKAFEKACGKTLPFEIAAR------RPGDIAACYA 287 L+ V N+ G ++ ++ + T P A+ R GD+ A Sbjct: 258 CTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFPHLRDAQPIFREFRAGDVRHSLA 317 Query: 288 DTALAKSTLGWEATRSLETMCADSWRW 314 D + LG+E + + +S W Sbjct: 318 DIGKGRRLLGYEPSHRIGRGLKESMNW 344 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 75.9 bits (185), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 87/322 (27%), Positives = 125/322 (38%), Gaps = 29/322 (9%) Query: 2 ELLKAGHQVVVFDNFCNSFPEAPRRVEKEXKKKITGKTLTLVKGDIRDRAAIEQALREHA 61 LL G QV DNF V E ++GDI D A +A Sbjct: 34 RLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAA-AWANFRFIEGDICDAATCLRAC--DG 90 Query: 62 CDAVIHFAGLKAVGESVVKPLEYYDNNVVGTVRLLEAMQAANVKTLVFSSSATVYGNPQY 121 D V+H A L +V S+ P+ NNV G + +L A + A V V+++S++ YG+ Sbjct: 91 ADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVARFVYAASSSTYGDHPG 150 Query: 122 LPLTES---HPLSASNPYGRSKIVIEDMLRDFHIAHPDWCIALLRYFNPVGAHESGLIGE 178 LP E PLS PY +K V E F + I LRYFN G + Sbjct: 151 LPKVEEVIGKPLS---PYAVTKYVNELYAEVFARCYGMESIG-LRYFNVFGRR------Q 200 Query: 179 DPQGIPNNLMPFVAQVAIGKRDKLNIWGGDYDTPDGTGVRDFIHVVDLAVGHLNALEYLK 238 DP G ++P I + +L I G DG RDF ++ ++ +L A + Sbjct: 201 DPHGAYAAVIPQWVTAMI-RNGELRING------DGQTSRDFCYIDNVVQANLLAATAGE 253 Query: 239 KPQCFAVNLGTGIGYSVLDVVKAFEKACGKTLPFEIAAR------RPGDIAACYADTALA 292 + N+ S+ D+ + + P R R GDI AD A Sbjct: 254 EAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPHLQEHRPSYVGFRAGDIRDSQADIGKA 313 Query: 293 KSTLGWEATRSLETMCADSWRW 314 + LG+ T L + W Sbjct: 314 RQRLGYAPTHRLRAGLEQAMSW 335 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,013,512 Number of extensions: 42609 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 7 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)