BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0049.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 238 3e-64 gi|300313683|ref|YP_003777775.1| dihydroneopterin aldolase [Herb... 235 4e-63 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 65 6e-12 gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 63 3e-11 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 55 7e-09 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 238 bits (608), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 124/171 (72%), Positives = 138/171 (80%) Query: 6 PSRQQVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQTLVAEIAALGRRAIALP 65 PS +ALVTGAA+RIGR IALALA+ GWD+AVHY +S EA TLV EI LGRRA+A+ Sbjct: 4 PSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRALAVQ 63 Query: 66 CELADEAGVRALVRQAGRLGPVACVVNNASLFREDRAESFSMAALDAHMRANVGAPVLLA 125 EL DE VR+L+ QA LG ++CVVNNASLF D A SF L HM ANVGAPVLLA Sbjct: 64 AELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPVLLA 123 Query: 126 QALYDATPEGRQAVVINLLDQKLYNPNPDFLSYTLSKAALQSATTLLAQAL 176 QALY+ATP+G+QAVVINLLDQKLYNPNPDFLSYTLSKAAL SATTLLAQAL Sbjct: 124 QALYEATPQGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQAL 174 >gi|300313683|ref|YP_003777775.1| dihydroneopterin aldolase [Herbaspirillum seropedicae SmR1] Length = 131 Score = 235 bits (599), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 109/128 (85%), Positives = 116/128 (90%) Query: 190 MFSLSHPELNDCRRLFLRNYEVLINIGVHEFEKKGEQRILINVDLFIPLAMSTPEHDQLQ 249 M LSHP L DCRRLFLRNYEV INIGVHEFEKKGEQRILINVDL++PLA +TP D L Sbjct: 1 MLFLSHPSLQDCRRLFLRNYEVFINIGVHEFEKKGEQRILINVDLYVPLAETTPTADHLD 60 Query: 250 EVVDYDFIRSTIAKRMAQGHIHLQETLCDDVVKAMLAHPKVRAVRVSTEKPDVYPDCDSV 309 EVVDYDFIRSTIA+RMA+GHIHLQETLCDDV+ AMLAHPKVRAVRVSTEKPDVYPDCDSV Sbjct: 61 EVVDYDFIRSTIAQRMARGHIHLQETLCDDVMTAMLAHPKVRAVRVSTEKPDVYPDCDSV 120 Query: 310 GVEVFRVK 317 GVE FR+K Sbjct: 121 GVETFRIK 128 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 65.5 bits (158), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 60/179 (33%), Positives = 92/179 (51%), Gaps = 8/179 (4%) Query: 10 QVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQTLVAEIAALGRRAIALPCELA 69 +V LVTG ++ IG AL + G+ +AV+Y + A A +VA+I A G RAIA+ ++A Sbjct: 6 KVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDVA 65 Query: 70 DEAGVRALVRQA-GRLGPVACVVNNASLF-REDRAESFSMAALDAHMRANVGAPVLLAQA 127 E V L G LG V +VNNA + + R E S ++ + ANV +L A+ Sbjct: 66 REEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCARE 125 Query: 128 LYD--ATPEGRQ-AVVINL--LDQKLYNPNPDFLSYTLSKAALQSATTLLAQALVTXFI 181 +T G Q ++NL + KL P +++ Y SK A+ + T LA+ + I Sbjct: 126 AVKRMSTRHGGQGGAIVNLSSMAAKLGGPG-EYVDYAASKGAIDAFTIGLAKEVAAEGI 183 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 53/172 (30%), Positives = 83/172 (48%), Gaps = 9/172 (5%) Query: 10 QVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQTLVAEIAALGRRAIALPCELA 69 ++AL+TG ++ +G+ AL LARDG + + Y + A+ VAE+ ALG A AL +LA Sbjct: 9 RIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALRLDLA 68 Query: 70 DEAGVRAL---VRQAGRLGPVA----CVVNNASLFREDRAESFSMAALDAHMRANVGAPV 122 D A A VR+A + A +VNN + E S A DA + ++ P Sbjct: 69 DSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDALFKVHLKGPF 128 Query: 123 LLAQALYDATPEGRQAVVINLLDQKLYNPNPDFLSYTLSKAALQSATTLLAQ 174 L QAL +G + +IN+ P + +Y K ++ T +A+ Sbjct: 129 FLTQALLPLLAQGGR--IINISSGLARFSLPGYAAYASMKGGVEVMTRYMAK 178 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 55.5 bits (132), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 52/178 (29%), Positives = 85/178 (47%), Gaps = 12/178 (6%) Query: 10 QVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQTLVAEIAALGRRAIALPCELA 69 +VAL+TG IGR +ALA AR+G +A+ Y +A+ ++ AL + LP ++ Sbjct: 126 KVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVG 185 Query: 70 D----EAGVRALVRQAGRLGPVACVVNNAS-LFREDRAESFSMAALDAHMRANVGAPVLL 124 + VRA++ GRL +VNNA+ + E S A L+ R N+ + + Sbjct: 186 SPEFCRSAVRAVMDAYGRLD---VLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 242 Query: 125 AQALYDATPE-GRQAVVINLLDQKLYNPNPDFLSYTLSKAALQSATTLLAQALVTXFI 181 + +A P R A +IN Y + + Y +K A+ + T L+Q L + I Sbjct: 243 VK---EAMPFLTRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGI 297 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 855,509 Number of extensions: 31843 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 9 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 130 (54.7 bits)