BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0042.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 666 0.0 gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 381 e-107 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 241 7e-65 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 93 3e-20 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 666 bits (1719), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/420 (79%), Positives = 381/420 (90%), Gaps = 2/420 (0%) Query: 1 VVNISVTGKAKRVSDEASDDVDPNDPFSEFFKRFGPQFQQQPRTPQIMRGLGSGFIISPD 60 VVNISVTGKA++V+D+ + +DPNDPFSEFFKRFGPQ Q P+ P+IM GLGSGFIISPD Sbjct: 76 VVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQM-PQQPRIMHGLGSGFIISPD 134 Query: 61 GLILTNAHVVDGAQEVTVKLTDRREFKAKVLGADKQSDIAVIRIPAKDLPTVQIGNPALV 120 GLILTNAHVVDGAQEV VKLTDRREFKAKVLG DKQSDIAVIRI AK+LPTVQIG+P+ V Sbjct: 135 GLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSRV 194 Query: 121 KVGEPVLAIGSPYGFDNTATAGIVSAKSRSLPDDNYVPFIQTDVAVNPGNSGGPLFNLKG 180 KVG+PVLAIGSPYGFDNTATAGI+SAKSRSLPDDNYVPFIQTDVAVNPGNSGGPLF+L G Sbjct: 195 KVGQPVLAIGSPYGFDNTATAGIISAKSRSLPDDNYVPFIQTDVAVNPGNSGGPLFDLNG 254 Query: 181 QVVGINSQIYSQTGGYQGLSFSIPIDVAMKVEQQLVQHGKVTRGRLGVSVQEINQALAES 240 QV+GINSQIYSQTGG+QGLSFSIPIDVAMKVEQQLV HGKVTRGRLGVSVQ++NQAL++S Sbjct: 255 QVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGVSVQDLNQALSDS 314 Query: 241 FGLKKSEGALVSSVEKGSPAEKAGVQPGDVILRFNGQAIDHSVDLPTLVADAVPGTTQKI 300 FGLKK+EGALVSSVEKGSPA+KAG+Q GDVI+ FNG AIDHSVDLPTLVAD PG+T+ + Sbjct: 315 FGLKKAEGALVSSVEKGSPADKAGLQAGDVIISFNGHAIDHSVDLPTLVADTPPGSTKPM 374 Query: 301 DVMRNGTLKSLNITIGELKPAK-EAAASRNEPQGRLGLAVRPLDKSEQQQIGVHGGLLVE 359 +VMR G ++++N+T+GE+K AK +A AS+++ QGRLGLAVRPLDK EQ Q+G GLLVE Sbjct: 375 EVMRAGKVRTINVTVGEMKQAKNDAKASKSDDQGRLGLAVRPLDKDEQAQLGGQKGLLVE 434 Query: 360 DVNGPAAVAGIQSGDVILSLNGNAVTSVEQLRSLVGKAGKSVALLVERGDDKIFVPVYLN 419 DV+GPAAVAGIQSGDVIL++NG V+SV QLR L AGK+VALLVERGD+KIFVP+ LN Sbjct: 435 DVSGPAAVAGIQSGDVILAVNGTPVSSVAQLRKLATDAGKNVALLVERGDEKIFVPLALN 494 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 381 bits (978), Expect = e-107, Method: Compositional matrix adjust. Identities = 219/444 (49%), Positives = 295/444 (66%), Gaps = 31/444 (6%) Query: 1 VVNISVTGKAKRVSDEASDDVDPNDP-FSEFFKRFG----PQFQQQPRTP---------- 45 VVNI T +RV A +DP EFF+RF P+ QQQP TP Sbjct: 52 VVNIRTT---ERVRQNAQGQGGVDDPEMQEFFRRFFGIPIPR-QQQPGTPRGNGKQGGQG 107 Query: 46 ---QIMRGLGSGFIISPDGLILTNAHVVDGAQEVTVKLTDRREFKAKVLGADKQSDIAVI 102 ++ RG+GSGFIIS DG I+TNAHVV+GA EV V LTD+REFKAK++G+D ++D+AV+ Sbjct: 108 QQEEVPRGVGSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVL 167 Query: 103 RIPAKDLPTVQIGNPALVKVGEPVLAIGSPYGFDNTATAGIVSAKSRSLPDDNYVPFIQT 162 +I +LP + +G+ ++VGE VLAIGSP+G +NT TAGIVSAK+R D Y+P IQT Sbjct: 168 KIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGD--YLPLIQT 225 Query: 163 DVAVNPGNSGGPLFNLKGQVVGINSQIYSQTGGYQGLSFSIPIDVAMKVEQQLVQHGKVT 222 DVAVNPGNSGGPL NLKG+V+GINSQIYS++GG+ G+SF++PID A++V QL G+VT Sbjct: 226 DVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVT 285 Query: 223 RGRLGVSVQEINQALAESFGLKKSEGALVSSVEKGSPAEKAGVQPGDVILRFNGQAIDHS 282 RGR+GV + E+ + +AES GL +++GALV VE G PAEKAG++ GD+IL++NG AI+ Sbjct: 286 RGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERP 345 Query: 283 VDLPTLVADAVPGTTQKIDVMRNGTLKSLNITIGEL-----KPAKEAAASRNEPQGRLGL 337 DLP +V PG + + R G+ + +++T+ EL K A+E A + LGL Sbjct: 346 SDLPRMVGSTKPGAKATVSIWRKGSARDVSVTVVELEADKPKQAEEKKAKPDNAPNSLGL 405 Query: 338 AVRPLDKSEQQQIGVHGGLLVEDVNGPAAVAGIQSGDVILSLNGNAVTSVEQLRSLVGK- 396 V L ++++++ V G+LVE V+G AA AGI+ GDVI LN V +Q LV K Sbjct: 406 VVSDLSDAQRKELKVDNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKL 465 Query: 397 -AGKSVALLVERGDDKIFVPVYLN 419 A K A+LV RGD FVP+ N Sbjct: 466 DAKKRAAVLVRRGDSSQFVPLRPN 489 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 241 bits (615), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 134/321 (41%), Positives = 197/321 (61%), Gaps = 9/321 (2%) Query: 1 VVNISVTGKAKRVSDEASDDVDPNDPFSEFFKRFGPQFQQQPRTPQIMRGLGSGFIISPD 60 VVNI T +A+ NDPF F++F + + LGSG I+SP Sbjct: 71 VVNIFTTTEARPQKSPFQ-----NDPF---FRKFFGDQFDEQQQDDKQSSLGSGVIVSPQ 122 Query: 61 GLILTNAHVVDGAQEVTVKLTDRREFKAKVLGADKQSDIAVIRIPAKDLPTVQIGNPALV 120 G ILTN HVV+ A ++ V L D R+ AKV+G D ++D+AVI+I +LP + +G+P Sbjct: 123 GYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPENS 182 Query: 121 KVGEPVLAIGSPYGFDNTATAGIVSAKSRS-LPDDNYVPFIQTDVAVNPGNSGGPLFNLK 179 VG+ VLAIG+P+G T T GIVSA R+ L + + FIQTD A+NPGNSGG L + Sbjct: 183 SVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVDTN 242 Query: 180 GQVVGINSQIYSQTGGYQGLSFSIPIDVAMKVEQQLVQHGKVTRGRLGVSVQEINQALAE 239 G ++GIN+ IYS+TGG G+ F+IP+ A V + ++ HG+V RG +GV Q+I LAE Sbjct: 243 GNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELAE 302 Query: 240 SFGLKKSEGALVSSVEKGSPAEKAGVQPGDVILRFNGQAIDHSVDLPTLVADAVPGTTQK 299 SFGL K GA+++ V KG PA++AG++PGD+++ + + + + ++ ++A PG Sbjct: 303 SFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQIA 362 Query: 300 IDVMRNGTLKSLNITIGELKP 320 + V+R LNIT+G+ P Sbjct: 363 MTVLRKSQETKLNITVGKRPP 383 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 70/173 (40%), Positives = 98/173 (56%), Gaps = 15/173 (8%) Query: 52 GSGFIISPDGLILTNAHVVDGAQEVTVKLTDRREFKAKVLGADKQSDIAVIRIP---AKD 108 G+GF I G +L+N HVV A+ V V+L RE A+VL D D+A+++ + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 109 LPTVQIGNPALVKVGEPVLAIGSPYG--FDNTATAGIVSAKSRSLPDDNYVPFIQTDVAV 166 LP +++ P L GE V A+GSP G F+ T T GI+S R L N F+Q+DVA+ Sbjct: 331 LP-LRLDEPGL---GEDVYALGSPLGDTFNTTLTRGILSG-VRLL---NGQEFLQSDVAI 382 Query: 167 NPGNSGGPLFNLKGQVVGINSQIYSQTGGYQGLSFSIPIDVAM-KVEQQLVQH 218 PG+SGGPL + GQVVGI G G++F IP+ A+ ++ QL Q Sbjct: 383 LPGSSGGPLLDRSGQVVGITVAGLGAR-GMAGMNFFIPLASAVERLNLQLQQR 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.135 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,368,052 Number of extensions: 62010 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 4 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)