BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0066.0041.p
         (198 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium...   318   2e-88
gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium...    74   9e-15
gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans...    57   1e-09

>gi|300313677|ref|YP_003777769.1| glutathione-regulated
           potassium-efflux system transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 606

 Score =  318 bits (814), Expect = 2e-88,   Method: Compositional matrix adjust.
 Identities = 156/198 (78%), Positives = 174/198 (87%), Gaps = 1/198 (0%)

Query: 1   VIIAGFGRFGQIVGRLLHANGIGLTILDHDPDQVESLRKFGFKVFYGDATRIDLLRTAGA 60
           VIIAGFGRFGQI+GRLL ANGIG T+LDHDPDQ++ LRKFGFKVFYGDATRIDLLRTAGA
Sbjct: 402 VIIAGFGRFGQIIGRLLAANGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGA 461

Query: 61  AKARLIVIAIDDAPASLKLVEAVQRAFPHLKIAARARNVTHLYDLMDRGVTVLERETFES 120
            KAR++VIAIDDA A+L++V+ V+  FPHL+IAARARNVTH YDLMDRGVTVLERETFES
Sbjct: 462 DKARVLVIAIDDAQAALEMVDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFES 521

Query: 121 ALQMGRQVLRQLDFGAYRAHRAAMKFRAHNIETLHKLYPHYKDQQQMVSMAVQAREELEA 180
           AL MGRQVL+QLDFGAYRA  AAMKFRAHN++TL  LYPHYK+Q QMVSMAVQAREELEA
Sbjct: 522 ALHMGRQVLQQLDFGAYRAREAAMKFRAHNLKTLEALYPHYKNQAQMVSMAVQAREELEA 581

Query: 181 MFASDNDAISDGDKH-RW 197
           MFA D  A+     H +W
Sbjct: 582 MFAGDRQAMEREHSHDQW 599


>gi|300313254|ref|YP_003777346.1| glutathione-regulated
           potassium-efflux system K+/H+ antiporter transmembrane
           protein [Herbaspirillum seropedicae SmR1]
          Length = 663

 Score = 73.6 bits (179), Expect = 9e-15,   Method: Compositional matrix adjust.
 Identities = 47/132 (35%), Positives = 66/132 (50%)

Query: 1   VIIAGFGRFGQIVGRLLHANGIGLTILDHDPDQVESLRKFGFKVFYGDATRIDLLRTAGA 60
           VIIAGFGR GQ + RLL   GI    L+ DP+ V+  R  G  V YGDA R + L  AG 
Sbjct: 417 VIIAGFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGI 476

Query: 61  AKARLIVIAIDDAPASLKLVEAVQRAFPHLKIAARARNVTHLYDLMDRGVTVLERETFES 120
            +A  +V+     P++LK++       P L +  R+ +   L  L+  G T +  E  E 
Sbjct: 477 HRAAALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEG 536

Query: 121 ALQMGRQVLRQL 132
           +L +    L  L
Sbjct: 537 SLMLASHALVML 548


>gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux
           transporter NAD-binding protein [Herbaspirillum
           seropedicae SmR1]
          Length = 546

 Score = 56.6 bits (135), Expect = 1e-09,   Method: Compositional matrix adjust.
 Identities = 38/130 (29%), Positives = 69/130 (53%), Gaps = 3/130 (2%)

Query: 1   VIIAGFGRFGQIVGRLLHANGIGLTILDHDPDQVESLRKFGFKVFYGDATRIDLLRTAGA 60
           V++ GFGR G+ +G  L A G    +++   + +E+LR  G    YG+A + +LL+ A  
Sbjct: 415 VVLVGFGRVGRGIGEQLRAQGKPFVVIEAQLENLEALRAAGVPALYGNAAQSELLQAAAI 474

Query: 61  AKARLIVIAIDDAPASLKLVEAVQRAFPHLKIAARARN---VTHLYDLMDRGVTVLERET 117
            +AR +++AI +   + +++E      P L++ ARA +   + HL     + V + ERE 
Sbjct: 475 DRARWLLVAIPEVFEAGQVIENALELNPGLQVVARAHSDAEIEHLEKHGAQRVIMGEREI 534

Query: 118 FESALQMGRQ 127
               L++  Q
Sbjct: 535 ARGMLELVGQ 544


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.138    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 588,523
Number of extensions: 21808
Number of successful extensions: 118
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 118
Number of HSP's successfully gapped: 3
Length of query: 198
Length of database: 1,619,870
Length adjustment: 80
Effective length of query: 118
Effective length of database: 1,241,070
Effective search space: 146446260
Effective search space used: 146446260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 128 (53.9 bits)