BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0040.p (276 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 419 e-119 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 113 2e-26 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 97 2e-21 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 66 3e-12 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 419 bits (1078), Expect = e-119, Method: Compositional matrix adjust. Identities = 225/264 (85%), Positives = 237/264 (89%) Query: 12 DPKRLWSLRRHIFGWGSAQVGLVSLGLFGAALAVGIDWRVALIGALGMSLSSTAIALATL 71 DPKRLW+LRR IFGWGS QV VSL L AALA + WR+ LIGALGMSLSSTAIALATL Sbjct: 74 DPKRLWALRRSIFGWGSIQVAAVSLALLAAALAASVPWRIGLIGALGMSLSSTAIALATL 133 Query: 72 GERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLLGVAVAQGSGQGWLHTLQVVAVIALL 131 GERKLTATPAGSA F+ILLFQDIAAIPMMAAVPLLGVA +QGSGQGWLH QV VIALL Sbjct: 134 GERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGWLHAGQVALVIALL 193 Query: 132 IFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLLMQSVGMSMALGTFLAGVLLADS 191 IFGGR L+RPILRIIAKTDMREIFTAF+LLLVIATCLLMQSVGMSMALGTFLAGVLLADS Sbjct: 194 IFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMALGTFLAGVLLADS 253 Query: 192 EYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLEQPWLILGMVLGFLVIKTVVLYGLA 251 EYRHALESDLEPFKGLLLGLFFIAVGMSVDFG+F QPW ILGMVLGFL IKT V++ L Sbjct: 254 EYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLGFLAIKTCVMWALG 313 Query: 252 KCFRIPRDQQIFFAFLLSQGGEFA 275 +CF IPR QQ+FFAFLLSQGGEFA Sbjct: 314 RCFGIPRGQQLFFAFLLSQGGEFA 337 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 97/288 (33%), Positives = 158/288 (54%), Gaps = 20/288 (6%) Query: 1 VLLLFLIGLELDPKRLWSLRRHIFGWGSAQVGL-----VSLGLFGAALAV----GIDWRV 51 V L+F IGLE +L ++R +FG G AQV + LG +GAA + I W+ Sbjct: 63 VFLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLG-WGAATFLPQFFNISWQA 121 Query: 52 ALIGALGMSLSSTAIALATLGERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLLGVAVA 111 A +++SSTAI L ER T G +LLFQD+A +P++ VP A+A Sbjct: 122 AFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVP----ALA 177 Query: 112 QGSGQ-----GWLHTLQVVAVIALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIAT 166 SG GW +V + LL FG + L+R RI+ K +E+F LL+ + Sbjct: 178 NESGNIMVTLGWAAGKALVVLALLLFFGSK-LMRGWFRIVVKRRSQELFMLNLLLITLGA 236 Query: 167 CLLMQSVGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFL 226 + + G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ + Sbjct: 237 AWITEKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVI 296 Query: 227 EQPWLILGMVLGFLVIKTVVLYGLAKCFRIPRDQQIFFAFLLSQGGEF 274 + +L+L ++ G +++K ++ LA+ F + L+Q GEF Sbjct: 297 DHWFLVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEF 344 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 93/287 (32%), Positives = 143/287 (49%), Gaps = 14/287 (4%) Query: 1 VLLLFLIGLELDPKRLWSLRRHIFGWGSAQVGLVSLGLFGAALAVGIDWRVA--LIGALG 58 +LL+F +GL K L S++ AQ+ + +L G AL + W V + L Sbjct: 66 ILLMFGVGLHFSLKDLLSVKNIAIPGAVAQITIATL--LGMALGWLLGWPVGEGFLFGLA 123 Query: 59 MSLSSTAIALATLGERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLL-GVAVAQG---S 114 +S++ST + L L ER+L T G L+ +DIA + + +P L G+ +G S Sbjct: 124 LSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALS 183 Query: 115 GQGWLHTL-----QVVAVIALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLL 169 G L TL +VVA A+++ GR +I IL IA T RE+F L + + L Sbjct: 184 GNAVLLTLGITLGKVVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALG 243 Query: 170 MQSV-GMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLEQ 228 V G+S ALG F AG++L++SE H + P + LFF++VGM D I + Sbjct: 244 SAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVARE 303 Query: 229 PWLILGMVLGFLVIKTVVLYGLAKCFRIPRDQQIFFAFLLSQGGEFA 275 P L+L VL +V K++ + + F P + + L+Q GEF+ Sbjct: 304 PLLVLATVLIIVVGKSLGALLIVRAFGYPNGTALTISASLAQIGEFS 350 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 58/230 (25%), Positives = 115/230 (50%), Gaps = 6/230 (2%) Query: 1 VLLLFLIGLELDPKRLWSLRRHIFGWGSAQVGLVSLGLFGAALAVGIDWR---VALIGAL 57 + LLF +GLE K+L + F +A++ L + G + + W+ +GA+ Sbjct: 68 IFLLFSLGLEFSLKKLAKVGATAFIGAAAEILL--MIWIGYEIGLYFGWKRMDAIFLGAM 125 Query: 58 GMSLSSTAIALATLGERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLLGVAVAQGSGQG 117 +++SST I + L E + F IL+ +DI AI M+A + + + + S + Sbjct: 126 -LAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVDSTEV 184 Query: 118 WLHTLQVVAVIALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLLMQSVGMSM 177 + ++V + + + G + +L I++ E+ L ++ CLL+ + S+ Sbjct: 185 FTTVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKLQYSV 244 Query: 178 ALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLE 227 ALG FL G ++A+S + H +E +EP + + +FF+A+G+ D + ++ Sbjct: 245 ALGAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVK 294 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.146 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 904,852 Number of extensions: 41558 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 4 Length of query: 276 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 192 Effective length of database: 1,222,130 Effective search space: 234648960 Effective search space used: 234648960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)