BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0037.p (89 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313674|ref|YP_003777766.1| hypothetical protein Hsero_4391... 115 7e-28 gi|300310527|ref|YP_003774619.1| transmembrane protein [Herbaspi... 102 4e-24 >gi|300313674|ref|YP_003777766.1| hypothetical protein Hsero_4391 [Herbaspirillum seropedicae SmR1] Length = 102 Score = 115 bits (288), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 78/89 (87%), Positives = 84/89 (94%) Query: 1 MKTLVKDAQELFREAAAATGDKADELRSKGLTMLEAAVEAAQNAQAVAIEKGKEVAAATD 60 MKTLVKDAQ LF EAAAATG+KADELR++GLT+LEAA EAAQ+AQA AIEKGKEVAA TD Sbjct: 14 MKTLVKDAQALFAEAAAATGEKADELRARGLTLLEAAAEAAQSAQAAAIEKGKEVAAKTD 73 Query: 61 DYVHENPWRAVAISAGVGLLVGLVIGRSK 89 DYVHENPWRAVAISAG+GLLVGLVIGRSK Sbjct: 74 DYVHENPWRAVAISAGLGLLVGLVIGRSK 102 >gi|300310527|ref|YP_003774619.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 144 Score = 102 bits (255), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 45/88 (51%), Positives = 65/88 (73%) Query: 1 MKTLVKDAQELFREAAAATGDKADELRSKGLTMLEAAVEAAQNAQAVAIEKGKEVAAATD 60 +++L++ AQ++F EAA ATG KAD+LR+ +LE A+ AQ+AQA ++ G+++ TD Sbjct: 53 VQSLLQQAQDMFAEAANATGKKADDLRASAQELLEQALNTAQDAQAAVVDTGRQIVTTTD 112 Query: 61 DYVHENPWRAVAISAGVGLLVGLVIGRS 88 DYV NPWRA+AI G+GLLVGL +GR Sbjct: 113 DYVQSNPWRAIAIGTGIGLLVGLAVGRC 140 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.126 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 172,719 Number of extensions: 3634 Number of successful extensions: 28 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 28 Number of HSP's successfully gapped: 2 Length of query: 89 Length of database: 1,619,870 Length adjustment: 59 Effective length of query: 30 Effective length of database: 1,340,505 Effective search space: 40215150 Effective search space used: 40215150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 123 (52.0 bits)