BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0064.0002.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309895|ref|YP_003773987.1| methionine synthase II protein ... 58 1e-09 >gi|300309895|ref|YP_003773987.1| methionine synthase II protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 57.8 bits (138), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 38/136 (27%), Positives = 64/136 (47%), Gaps = 7/136 (5%) Query: 168 PTTTIGSFPQTAEIRHARSEFKAGRLHTAAYKTAMQAEIERSVREQETLGLDVLVHGEAE 227 PT+T GS P+ A + + +L A + A + + S++EQ+ G+D++ GE Sbjct: 6 PTSTAGSLPKPAWLAEPEKLWSPWKLQGAELEDAKRDALRLSLQEQQHAGIDIVSDGEQT 65 Query: 228 RNDMVEYFGEQLQGYAFSQFGWVQSYGSRCVKPPVLFGDISRPRAMTVEWITYAQSLTAK 287 R V F E L G F++ V+ P + G +SRP+ + V+ + +S T + Sbjct: 66 RQHFVTTFIEHLDGVDFAKRETVRIRNRYDASVPTVVGAVSRPKPVFVDDARFLRSQTDQ 125 Query: 288 PMKXQQKSRLWTLISP 303 P+K W L P Sbjct: 126 PIK-------WALPGP 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 981,000 Number of extensions: 37058 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 1 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)