BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0063.0002.p (154 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312591|ref|YP_003776683.1| tRNA pseudouridine synthase [He... 183 6e-48 >gi|300312591|ref|YP_003776683.1| tRNA pseudouridine synthase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 183 bits (464), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 93/150 (62%), Positives = 107/150 (71%), Gaps = 17/150 (11%) Query: 22 ARARXTLADIGTTCAGRTDSGVHALEQVVHFDTVIARETASWVRGVNAFLPPSIAVRWAC 81 A R + I TTCAGRTDSGVHALEQ+VHFDT IAR++ SWVRG NAFLPP+IAVRWAC Sbjct: 44 ALQRFSTQPIATTCAGRTDSGVHALEQIVHFDTPIARDSFSWVRGTNAFLPPTIAVRWAC 103 Query: 82 EVPLEEGVG----------INAGER-------EDGFHARFSATARTYHYVLYNHPVRSPL 124 E+P + ++ +R + GFHARFSA ARTYHYVLYNH VR+PL Sbjct: 104 ELPYVDDAAIASAASQAAELHGADRAGADSLSQFGFHARFSAIARTYHYVLYNHAVRTPL 163 Query: 125 LAGKAGWTFRPLDAGLMQQGADYLIGMHDF 154 LAGKAGWTFRPLDA M + A +LIG HDF Sbjct: 164 LAGKAGWTFRPLDADRMHRAAQHLIGEHDF 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 468,153 Number of extensions: 18707 Number of successful extensions: 35 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 34 Number of HSP's successfully gapped: 1 Length of query: 154 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 77 Effective length of database: 1,255,275 Effective search space: 96656175 Effective search space used: 96656175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)