BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0061.0003.p (411 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 651 0.0 gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 369 e-103 gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 223 3e-59 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 651 bits (1680), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/401 (86%), Positives = 372/401 (92%) Query: 11 FXDFGQSLKPLGDGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVVTT 70 + DFGQ+LKPLGDGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVVTT Sbjct: 27 WPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVVTT 86 Query: 71 IALLLGLVLAFVFQPGAGMNIDPTNLDASGLSSYVETAGKVRGTSFADFIMKLIPSTIVS 130 IALLLGLVLA V QPGAGMNIDP+NLDAS LS Y+ AGKV+ T FA+FIMKLIP+T VS Sbjct: 87 IALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYMANAGKVKETGFAEFIMKLIPATAVS 146 Query: 131 AFTSGDVLQVLLISVIFGCALLLIGDKGQPAVSLIASLSEAFFKCMAFIIRLAPLGVFGA 190 AFTSGDVLQVLLISV FGCALLLIG+KG P V+L+ASLS+AFFKCM+F IRLAPLGV GA Sbjct: 147 AFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALVASLSDAFFKCMSFFIRLAPLGVLGA 206 Query: 191 IAFTVGRYGIGSLKQLGMLVLLFYGAVIFFVVVVLGAILRTSGLSIFKLITYLREELIVV 250 IAFTVG+YGIGSLKQL +LVLLFYGAVIFFV+VVLG ILR SGLSIFKLI YLREEL+VV Sbjct: 207 IAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVLGGILRASGLSIFKLIRYLREELVVV 266 Query: 251 LATASSDSVLPQVMRKLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAAVFIAQATNTH 310 LAT SSDSVLPQ+M+KLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAA+FIAQATNTH Sbjct: 267 LATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAALFIAQATNTH 326 Query: 311 LAMGDLLMILAIALITSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGIMR 370 L+MGDL ILAIAL+TSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGI R Sbjct: 327 LSMGDLAAILAIALVTSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGIAR 386 Query: 371 ALGNLLGNCVATVVVATWEKDIDKTRARAVLNGEIPAATFE 411 ALGNLLGNCVATVV+A+WE+DIDK RARAVLNGE A E Sbjct: 387 ALGNLLGNCVATVVIASWERDIDKVRARAVLNGESVAPLVE 427 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 369 bits (948), Expect = e-103, Method: Compositional matrix adjust. Identities = 201/396 (50%), Positives = 277/396 (69%), Gaps = 3/396 (0%) Query: 9 RIFXDFGQSLKPLGDGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVV 68 + G+++KPLGDGF+KLIKMII ++FC VV GI G D+KKVG G A++YFEVV Sbjct: 28 HFYPSTGEAMKPLGDGFVKLIKMIIAPVIFCTVVIGIAGMEDMKKVGKTGGLALLYFEVV 87 Query: 69 TTIALLLGLVLAFVFQPGAGMNIDPTNLDASGLSSYVETAGKVRGTSFADFIMKLIPSTI 128 +T+AL++GL+L QPG GMN+DP +LD +++Y GK+ S DF++ +IP+++ Sbjct: 88 STVALIIGLLLVNFLQPGVGMNVDPASLDTKSIAAYT-APGKMG--SVTDFVLGIIPTSM 144 Query: 129 VSAFTSGDVLQVLLISVIFGCALLLIGDKGQPAVSLIASLSEAFFKCMAFIIRLAPLGVF 188 V AF GDVLQVLL++V+FG AL G +G I +S F + I+++AP+G F Sbjct: 145 VDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKISHVLFSVVGAIMKVAPIGAF 204 Query: 189 GAIAFTVGRYGIGSLKQLGMLVLLFYGAVIFFVVVVLGAILRTSGLSIFKLITYLREELI 248 GA++FT+G+YG+GSL L L+ FY + F+ VVLG I R G SI+K + Y++EEL+ Sbjct: 205 GAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGIITRLHGFSIWKFVKYIKEELL 264 Query: 249 VVLATASSDSVLPQVMRKLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAAVFIAQATN 308 +VL T+SS+SVLP+++ K+E+ G KK+ VGLVIPTGYSFNLD +IYLTMAAVFIAQATN Sbjct: 265 IVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQATN 324 Query: 309 THLAMGDLLMILAIALITSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGI 368 T + L +L + L+TSKGA G+ GS ++LAATL+ + +PV GL L+L ID F+ Sbjct: 325 TPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAVGHVPVAGLALILGIDRFMSE 384 Query: 369 MRALGNLLGNCVATVVVATWEKDIDKTRARAVLNGE 404 RAL N +GN VAT+VVA W ++D R VLN E Sbjct: 385 ARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNE 420 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 223 bits (567), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 131/372 (35%), Positives = 216/372 (58%), Gaps = 19/372 (5%) Query: 23 DGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLAFV 82 D F++LIKM+I +VF +V GI D K VG + K++ +F + + ++L LG+++A + Sbjct: 46 DLFLRLIKMVIAPLVFSTLVVGIAHMGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANL 105 Query: 83 FQPGAGMNIDPTNLDASGLSSYVETAGKVRGTSFADFIMKLIPSTIVSAFTSGDVLQVLL 142 QPGAG+ + + +GL++ T +F L+P +IV A +VLQV++ Sbjct: 106 LQPGAGVALPSPDAAGAGLATSKFTV--------KEFFNHLVPKSIVEAMAQNEVLQVVV 157 Query: 143 ISVIFGCALLLIGDKGQPAVSLIASLSEAFFKCMAFIIRLAPLGVFGAIAFTVGRYGIGS 202 S+ FG AL +G++G+ +++I LS K ++++ AP+ VF A+A TV G+ Sbjct: 158 FSMFFGIALASLGERGKHLLAVIDDLSHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEI 217 Query: 203 LKQLGMLVLLFYGAVIFFVVVVLGAILRTSGL-----SIFKLITYLREELIVVLATASSD 257 L + + FY F +V+L IL G IF L+ ++E ++ ATASS+ Sbjct: 218 LVSFAVFMRDFY-----FSLVLLWVILIAVGFIFLKKRIFHLLALIKEAFLLAFATASSE 272 Query: 258 SVLPQVMRKLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAAVFIAQATNTHLAMGDLL 317 + P+++ L+ G+K+ V+P GYSFNLD IY T A +FIAQA H+ + + Sbjct: 273 AAYPKLLDALDRFGVKRKISSFVMPMGYSFNLDGSMIYCTFATLFIAQAYGIHMPISTQI 332 Query: 318 MILAIALITSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGIMRALGNLLG 377 ++ + ++TSKG GVP +++V++AATL IP GL+++L ID F+ + R+ N +G Sbjct: 333 TMMLVLMLTSKGIAGVPRASLVVIAATLHQFN-IPEAGLLVILGIDTFLDMGRSATNAVG 391 Query: 378 NCVATVVVATWE 389 N +A+ VVA WE Sbjct: 392 NSIASAVVAKWE 403 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,146,072 Number of extensions: 45619 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 3 Length of query: 411 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 323 Effective length of database: 1,203,190 Effective search space: 388630370 Effective search space used: 388630370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)