BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0058.0016.p (278 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 131 8e-32 gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 128 5e-31 gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 126 2e-30 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 119 2e-28 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 86 2e-18 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 131 bits (329), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 71/235 (30%), Positives = 128/235 (54%), Gaps = 2/235 (0%) Query: 42 VVAVWEAIIRFFEIPIYIMPAPSNIALALVNTG--YLDNALYTLGEAMLGFLLAALFGIV 99 ++ VW+ I +++ +I P+P +I A+V+ ++AL T M+GF +A + G+ Sbjct: 25 ILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPITEHALSTFWVTMVGFAIAVVVGVT 84 Query: 100 LGGLIAQFPLAEKTLYPYLIAIQTTPKVAVAPLFIMWFGFGMTSKVIIAATIAFFPILVN 159 LG +I P+ YP + A PK A P+ ++WFG G ++ A I+FFPI+VN Sbjct: 85 LGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLISFFPIMVN 144 Query: 160 VISGLRSTDPARLELMRSLRATRWQIFTMVRLPGALPMIFAGLNIAIIFSILGAIVGEFL 219 + +GL + +P +++R L A R I V LP ++P FA L +AI + +G+ V E Sbjct: 145 IATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLAFVGSTVSEMN 204 Query: 220 GSRKGLGNAIMQMNVNLDTAGVFATLFVLSAIGVCLHVLMSFLQRKALFWADSSR 274 S +G+G ++ ++ FA L V+ A+ + ++ L + ++++ WA + Sbjct: 205 ASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAMYELFAIIEKRTTGWAHRGK 259 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 86/241 (35%), Positives = 131/241 (54%), Gaps = 7/241 (2%) Query: 40 VAVVAVWEAIIRFFEIPIYIMPAPSNI--ALALVNTGYLDNALYTLGEAMLGFLLAALFG 97 +A++ +W+ + E+P Y++P P+ I LA + + + L TL L F LA + G Sbjct: 25 IALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWSMLMASLLVTLKITFLAFALAVVLG 84 Query: 98 IVLGGLIAQFPLAEKTLYPYLIAIQTTPKVAVAPLFIMWFGFGMTSKVIIAATIAFFPIL 157 + + + Q E +L+PY I +Q TP VA+APL I+W + + A +A FPI+ Sbjct: 85 VAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVCATIMALFPII 144 Query: 158 VNVISGLRSTDPARLELMRSLRATRWQIFTMVRLPGALPMIFAGLNIAIIFSILGAIVGE 217 N GLRS +P L L R +ATRWQ +R+P ALP F GL I+ +++GA+V E Sbjct: 145 SNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSGLALIGAVVAE 204 Query: 218 FL----GSRKGLGNAIMQMNVNLDTAGVFATLFVLSAIGVCLHVLMSFLQRKALF-WADS 272 F+ G+ GL I+Q L+ +FA L +++ G+ L LMS L R L W +S Sbjct: 205 FVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITLTGILLFWLMSALSRAMLAGWHES 264 Query: 273 S 273 Sbjct: 265 E 265 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 126 bits (316), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 70/197 (35%), Positives = 111/197 (56%) Query: 77 DNALYTLGEAMLGFLLAALFGIVLGGLIAQFPLAEKTLYPYLIAIQTTPKVAVAPLFIMW 136 + L TL E +L F++ +FG+ +G +A P A L PY+ A + P+V +AP+F MW Sbjct: 81 SHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVILAPIFAMW 140 Query: 137 FGFGMTSKVIIAATIAFFPILVNVISGLRSTDPARLELMRSLRATRWQIFTMVRLPGALP 196 FG G+ SKV +A T+ FF + NV G++ P L R L A Q+ V LP A Sbjct: 141 FGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRTVYLPSATS 200 Query: 197 MIFAGLNIAIIFSILGAIVGEFLGSRKGLGNAIMQMNVNLDTAGVFATLFVLSAIGVCLH 256 +F+ L+ +I + +GA+VGE+LGS +G+G I+Q D VFA + VL+ + L Sbjct: 201 WVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVLTIFALVLD 260 Query: 257 VLMSFLQRKALFWADSS 273 + + ++++ + W S Sbjct: 261 LAVGVIEKRLMKWQPKS 277 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 119 bits (299), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 84/279 (30%), Positives = 142/279 (50%), Gaps = 10/279 (3%) Query: 1 MESVHNNPAAPNVTPASTSFSLRRTLRARPELL--LIPTVFVAVVAVWEAIIR------- 51 M + +A + A+ +R +RAR +L+ L + V V+ WE R Sbjct: 1 MTQAQASQSAATMDIAAVEAEAQRKIRARKQLVIGLRIAILVIVLGGWEVCARIGWIDPF 60 Query: 52 FFEIPIYIMPAPSNIALALVNTGYL-DNALYTLGEAMLGFLLAALFGIVLGGLIAQFPLA 110 FF P I+ + + + G L L TL E +LGFL+ ++ G++ G ++ + L Sbjct: 61 FFSQPSLIVAQIYDWMVEGTSQGPLWTQVLVTLEETVLGFLIGSVAGVIAGIVLGRNKLL 120 Query: 111 EKTLYPYLIAIQTTPKVAVAPLFIMWFGFGMTSKVIIAATIAFFPILVNVISGLRSTDPA 170 Y+ + P+V + +F++ FG GM SKV +A + FF + N G+R D Sbjct: 121 SDVFSLYIQIANSIPRVVLGSIFVIAFGLGMASKVALAVVMVFFVVFANAFQGVREADRY 180 Query: 171 RLELMRSLRATRWQIFTMVRLPGALPMIFAGLNIAIIFSILGAIVGEFLGSRKGLGNAIM 230 + + L A+R Q+ V +P AL I A L+++ F+++GA+VGEFLGS++G+G I Sbjct: 181 MIANAQILGASRRQVTMAVVIPSALSWILASLHVSFGFALVGAVVGEFLGSKQGIGLLIS 240 Query: 231 QMNVNLDTAGVFATLFVLSAIGVCLHVLMSFLQRKALFW 269 + +GVFA + VL+ + + L+ L+R+ L W Sbjct: 241 TAQGAFNASGVFAAMIVLAVVALAADWLLHALERRLLKW 279 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 86.3 bits (212), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 66/245 (26%), Positives = 121/245 (49%), Gaps = 7/245 (2%) Query: 34 LIPTVFVAVVAVWEAIIRFFEIPIYIMPAPSNIA---LALVNTGYL-DNALYTLGEAMLG 89 ++P F+ +V EA R IP ++P PS +A +L G L + +L G Sbjct: 30 IVPLAFLLLV---EAGARLELIPARLLPPPSELAHTLWSLAQRGELLPHIAASLARVAAG 86 Query: 90 FLLAALFGIVLGGLIAQFPLAEKTLYPYLIAIQTTPKVAVAPLFIMWFGFGMTSKVIIAA 149 F++ + + LG L+ AE L P A++ P +A PL ++W G T KV++ A Sbjct: 87 FVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIA 146 Query: 150 TIAFFPILVNVISGLRSTDPARLELMRSLRATRWQIFTMVRLPGALPMIFAGLNIAIIFS 209 AFFP+ +N+++G+R D +E+ + + + LP +LP +FAGL + + Sbjct: 147 IGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLFAGLRGGLSLA 206 Query: 210 ILGAIVGEFLGSRKGLGNAIMQMNVNLDTAGVFATLFVLSAIGVCLHVLMSFLQRKALFW 269 + + E + + KG+G + + V A + +L+ +G L+ +++K+L W Sbjct: 207 WMFLVAAELIAATKGVGYLLSDGRESSRPDLVLAAILILALLGKLSDGLLRAVEQKSLGW 266 Query: 270 ADSSR 274 ++ R Sbjct: 267 REARR 271 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 765,618 Number of extensions: 28492 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 14 Length of query: 278 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 194 Effective length of database: 1,222,130 Effective search space: 237093220 Effective search space used: 237093220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)