BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0056.0020.p (159 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311987|ref|YP_003776079.1| acyl-CoA thioester hydrolase [H... 287 3e-79 gi|300313155|ref|YP_003777247.1| acyl-CoA hydrolase [Herbaspiril... 80 1e-16 >gi|300311987|ref|YP_003776079.1| acyl-CoA thioester hydrolase [Herbaspirillum seropedicae SmR1] Length = 159 Score = 287 bits (735), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 133/157 (84%), Positives = 145/157 (92%) Query: 1 MDMPSHQLTMTVLMTPDMANFSGNVHGGTILKFLDQVAYACASRYAGQYVVTLSVDQVMF 60 MD+PSHQLTMTVLMTPDMANF+GNVHGGTILK LDQVAYAC SRYAGQYVVTLSVDQVMF Sbjct: 1 MDLPSHQLTMTVLMTPDMANFAGNVHGGTILKLLDQVAYACGSRYAGQYVVTLSVDQVMF 60 Query: 61 RQPIHVGELVSFLASVNHTGTSSMEVGIKVVAENIRTQEVRHVNSCFFTMVAVDGERKPV 120 RQPIHVGELV+FLASVNHTG SSME+GIKVVAENIRTQE RHVNSCFFTMVAVD +RKP+ Sbjct: 61 RQPIHVGELVTFLASVNHTGKSSMEIGIKVVAENIRTQETRHVNSCFFTMVAVDDQRKPI 120 Query: 121 AVPSLRPFTGEEKRRFEAAKLRKKLRAELSQRFEEAK 157 VP LRPF+ EEKRRFEAA +RK LR EL++R++E K Sbjct: 121 PVPPLRPFSQEEKRRFEAAVIRKSLRQELARRYDEVK 157 >gi|300313155|ref|YP_003777247.1| acyl-CoA hydrolase [Herbaspirillum seropedicae SmR1] Length = 144 Score = 79.7 bits (195), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 45/125 (36%), Positives = 66/125 (52%), Gaps = 1/125 (0%) Query: 4 PSHQLTMTVLMTPDMANFSGNVHGGTILKFLDQVAYACASRYAGQYVVTLSVDQVMFRQP 63 P + V+ P AN G+V GG I+ +D A+R A V T++V+ +F+ P Sbjct: 10 PGKAPQLRVMPMPSDANVYGDVFGGWIMAQVDIAGSLPATRRANGRVATIAVNSFLFKNP 69 Query: 64 IHVGELVSFLASVNHTGTSSMEVGIKVVAENIRTQ-EVRHVNSCFFTMVAVDGERKPVAV 122 + VG+L+SF A + G +S+ V ++V AE R Q E+ V T VA D RKP + Sbjct: 70 VFVGDLLSFYADIVKVGNTSITVNVEVYAERNRLQTEIVKVTEATLTYVATDENRKPRPL 129 Query: 123 PSLRP 127 P L P Sbjct: 130 PPLDP 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 432,928 Number of extensions: 15172 Number of successful extensions: 43 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 43 Number of HSP's successfully gapped: 2 Length of query: 159 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 81 Effective length of database: 1,250,540 Effective search space: 101293740 Effective search space used: 101293740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)