BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0053.0009.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 139 4e-34 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 135 5e-33 gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 94 2e-20 gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspiri... 83 4e-17 gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspiri... 80 3e-16 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 139 bits (350), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 77/183 (42%), Positives = 113/183 (61%), Gaps = 1/183 (0%) Query: 219 AEADEILAEVVDSTTEMCHDE-KISTLSLKRAGILASAPAGIALTSGSHLQLAAQEHFIA 277 A A + L + VD+T + + + S K+ ++ +APAGI L + Q+ + + Sbjct: 648 ALAQDSLKDFVDATEDKYSGQGQGSANRFKKPLLMMAAPAGIGLATDQGTQVHSAQETTL 707 Query: 278 TAGGNADISVMKRFTAAAGEAVSLFAQKLGMKLFAAKGKVEIQAQSDDINIISDRNMFIT 337 T+G + I+ K A E +SLFAQ GMKLFAAKGKVEIQAQSDD+++I+++ + Sbjct: 708 TSGKDTSIASGKSLLATVKEKISLFAQNAGMKLFAAKGKVEIQAQSDDLDLIAEKVARLL 767 Query: 338 SANGRVTVEAKDELLLKCGGSYIRINASGIEDGTPGASVVKAGSHGRPGPASLPPALPQL 397 S +GRV + AKDE+++ GGS+I+IN++GI GTPG KA H PGPAS +P + Sbjct: 768 STSGRVEIHAKDEVMISAGGSFIQINSAGITQGTPGKWEAKASLHSMPGPASQNFVMPHV 827 Query: 398 PKS 400 KS Sbjct: 828 QKS 830 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 135 bits (341), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 73/171 (42%), Positives = 108/171 (63%), Gaps = 1/171 (0%) Query: 221 ADEILAEVVDSTTEMCHD-EKISTLSLKRAGILASAPAGIALTSGSHLQLAAQEHFIATA 279 E L + +D+T D + ++A +LA++P GI LT+ + A ++ Sbjct: 640 GQEALRDFIDATQADYEDPSQAQANRFQQAILLAASPDGIGLTTPKGVHTHAGGEITLSS 699 Query: 280 GGNADISVMKRFTAAAGEAVSLFAQKLGMKLFAAKGKVEIQAQSDDINIISDRNMFITSA 339 G + I+V K A+ E +SLFA K G+KLF+AKGKVE+QAQSDD+ +I+++ + SA Sbjct: 700 GADTSIAVGKSLLASVAEKISLFAFKAGIKLFSAKGKVEVQAQSDDLELIAEKVARLLSA 759 Query: 340 NGRVTVEAKDELLLKCGGSYIRINASGIEDGTPGASVVKAGSHGRPGPASL 390 +GRV + AK+E+L+ GGS+IR+NASGI GT GA KAG+H PGP +L Sbjct: 760 SGRVEIRAKEEVLITAGGSFIRLNASGITQGTSGAWEAKAGTHAMPGPTTL 810 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 94.4 bits (233), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 57/145 (39%), Positives = 83/145 (57%), Gaps = 1/145 (0%) Query: 251 ILASAPAGIALTSGSHLQLAAQEHFIATAGGNADISVMKRFTAAAGEAVSLFAQKLGMKL 310 ++ S+P+GI +SG+ LA+ EH TAG + ++ K F A+A E +SL A +LG KL Sbjct: 658 LVLSSPSGIEASSGASTHLASAEHAALTAGSHISLAAGKSFFASAAEKLSLLAYRLGAKL 717 Query: 311 FAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKDELLLKCGGSYIRINASGIEDG 370 AA G+VEIQAQ+D + +++ + + I S + ++AK + L GGS + I A GI Sbjct: 718 IAASGRVEIQAQNDGMELLAQKVVDIISTRDWINLKAKKGIRLNGGGSELVI-AEGITGF 776 Query: 371 TPGASVVKAGSHGRPGPASLPPALP 395 T GA V A H GP P P Sbjct: 777 TQGAHHVHAADHQTLGPQGRPAEFP 801 >gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 913 Score = 82.8 bits (203), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 54/172 (31%), Positives = 94/172 (54%), Gaps = 5/172 (2%) Query: 251 ILASAPAGIALTSGSHLQLAAQEHFIATAGGNADISVMKRFTAAAGEAVSLFAQKLGMKL 310 + +APAG+A+TS + L AQ+ + GN S K+ A E++ LFAQ+LG++L Sbjct: 704 VAVTAPAGVAITSDEGVALGAQKEIDLISVGNTQFSAGKKLLARVAESICLFAQRLGIQL 763 Query: 311 FAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKDELLLKCGGSYIRINASGIEDG 370 AA GK+++Q D+ + S R + +T+A+ + ++A L L+ G+ I + I Sbjct: 764 VAANGKLQLQTHGGDVEVTSARRIVLTAAD-EIVLQAP-RLRLQAQGAQIDLGGGVITQQ 821 Query: 371 TPGASVVKAGS-HGRPGPASLPPALPQLPKSVCKECLKEALRARAKTSVPNI 421 + G V+++G+ H G S PP + +LP S + + L AR + P++ Sbjct: 822 SSGDHVIRSGNFHHVSGADSQPPGV-ELPASTMRTDERFIL-ARRGSGRPHV 871 >gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 912 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 46/150 (30%), Positives = 81/150 (54%), Gaps = 9/150 (6%) Query: 259 IALTSGSHLQLAAQEHFIATAGGNADISVMKR--------FTAAAGEAVSLFAQKLGMKL 310 +A+T+ + L + E + AG N D ++ TA AG+ +SLFAQ + L Sbjct: 700 VAVTAPAGLHFSTPETAVTHAGENCDTVALRHIQLASGEHLTANAGKGISLFAQSEDLTL 759 Query: 311 FAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKDELLLKCG-GSYIRINASGIED 369 A +G++++Q+Q +D + + +N+ +T++ G+V + A DE+LL GSY+R+ I+ Sbjct: 760 IAHQGELKLQSQHNDTKVAAAKNLSLTASGGKVQIIAADEILLAASDGSYLRLGGGKIDM 819 Query: 370 GTPGASVVKAGSHGRPGPASLPPALPQLPK 399 G GA+ + A +H + P LP K Sbjct: 820 GCSGAATIHAATHRWIDAQTTPGELPTFAK 849 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,228,531 Number of extensions: 48815 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 10 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)