BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0053.0008.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 183 2e-47 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 182 5e-47 gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 128 5e-31 gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspiri... 128 7e-31 gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspiri... 127 1e-30 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 183 bits (465), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 112/290 (38%), Positives = 167/290 (57%), Gaps = 16/290 (5%) Query: 43 NKLRMEDWKGQEHIKLSTEHSGKSQLNLGHLVDSQRKER----GQGVELRTDAHAAIRGG 98 N+L M+D Q + L + S +QLNLG+LV ER G G L TD + A+ Sbjct: 542 NQLVMDDTTSQNRVHLYSSQS-NAQLNLGYLVSQADNERRNFYGSGFALSTDDYGAVVTQ 600 Query: 99 KGVFINADAQPGAQGKQLDMQAAQTLLEQALQQTQSLAESAN--AAQALAADCEQQRALL 156 KG+++++ +PG QG QLD A +L++ + + L++SA+ A+ALA D + Sbjct: 601 KGLYLSSYGRPGPQGTQLDASEATSLMKASADLARGLSDSASRAGAEALAQDSLKDFVDA 660 Query: 157 NDTLLS---------LKRAGILASAPAGIALTSGSHLQLAAQEHFIATAGGNADISVMKR 207 + S K+ ++ +APAGI L + Q+ + + T+G + I+ K Sbjct: 661 TEDKYSGQGQGSANRFKKPLLMMAAPAGIGLATDQGTQVHSAQETTLTSGKDTSIASGKS 720 Query: 208 FTAAAGEAVSLFAQKLGMKLFAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKDE 267 A E +SLFAQ GMKLFAAKGKVEIQAQSDD+++I+++ + S +GRV + AKDE Sbjct: 721 LLATVKEKISLFAQNAGMKLFAAKGKVEIQAQSDDLDLIAEKVARLLSTSGRVEIHAKDE 780 Query: 268 LLLKCGGSYIRINASGIEDGTPGASVVKAGSHGRPGPASLPPALPQLPKS 317 +++ GGS+I+IN++GI GTPG KA H PGPAS +P + KS Sbjct: 781 VMISAGGSFIQINSAGITQGTPGKWEAKASLHSMPGPASQNFVMPHVQKS 830 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 182 bits (461), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 114/281 (40%), Positives = 166/281 (59%), Gaps = 17/281 (6%) Query: 43 NKLRMEDWKGQEHIKLSTEHSGKSQLNLGHLVDSQRKER----GQGVELRTDAHAAIRGG 98 N++ M+D Q I L + ++ +QLNLGHLV ER G G L TDA+ AI Sbjct: 531 NQMVMDDTPEQNRIHLYSTNT-HAQLNLGHLVSQTGNERRSFFGSGFALSTDAYGAIVTH 589 Query: 99 KGVFINADAQPGAQGKQLDMQAAQTLLEQALQQTQSLAESANAAQALA-ADCEQQRALLN 157 KG++I+ +PGAQG QLD + A L+ +++L+E+A A A A E R ++ Sbjct: 590 KGLYISTYGRPGAQGTQLDAREATGQLKSGANLSRTLSETAAKAGAEPLAGQEALRDFID 649 Query: 158 DTLLS-----------LKRAGILASAPAGIALTSGSHLQLAAQEHFIATAGGNADISVMK 206 T ++A +LA++P GI LT+ + A ++G + I+V K Sbjct: 650 ATQADYEDPSQAQANRFQQAILLAASPDGIGLTTPKGVHTHAGGEITLSSGADTSIAVGK 709 Query: 207 RFTAAAGEAVSLFAQKLGMKLFAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKD 266 A+ E +SLFA K G+KLF+AKGKVE+QAQSDD+ +I+++ + SA+GRV + AK+ Sbjct: 710 SLLASVAEKISLFAFKAGIKLFSAKGKVEVQAQSDDLELIAEKVARLLSASGRVEIRAKE 769 Query: 267 ELLLKCGGSYIRINASGIEDGTPGASVVKAGSHGRPGPASL 307 E+L+ GGS+IR+NASGI GT GA KAG+H PGP +L Sbjct: 770 EVLITAGGSFIRLNASGITQGTSGAWEAKAGTHAMPGPTTL 810 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 94/271 (34%), Positives = 136/271 (50%), Gaps = 28/271 (10%) Query: 66 SQLNLGHLVDSQRKE-----RGQGVELRTDAHAAIRGGKGVFINADAQPGAQGKQLDMQA 120 SQL LGH+ +R RG+G ELRTDAH +R +G+ + ++ +P A D+ Sbjct: 535 SQLALGHITRIERHAGRQDARGEGFELRTDAHGVVRAAQGLLLTSEPRPKAHRHTTDIGE 594 Query: 121 AQTLLEQALQQTQSL-------------AESANAAQALAADCEQQRALLND------TLL 161 L QA +SL A+ +AAQA+ A Q A+ D Sbjct: 595 TVARLTQARGTVESLSKLAQEHEAQDKNADQYDAAQAIKA---QNDAIKGDGAAKGGRFP 651 Query: 162 SLKRAGILASAPAGIALTSGSHLQLAAQEHFIATAGGNADISVMKRFTAAAGEAVSLFAQ 221 L ++ S+P+GI +SG+ LA+ EH TAG + ++ K F A+A E +SL A Sbjct: 652 ELTEPHLVLSSPSGIEASSGASTHLASAEHAALTAGSHISLAAGKSFFASAAEKLSLLAY 711 Query: 222 KLGMKLFAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKDELLLKCGGSYIRINA 281 +LG KL AA G+VEIQAQ+D + +++ + + I S + ++AK + L GGS + I A Sbjct: 712 RLGAKLIAASGRVEIQAQNDGMELLAQKVVDIISTRDWINLKAKKGIRLNGGGSELVI-A 770 Query: 282 SGIEDGTPGASVVKAGSHGRPGPASLPPALP 312 GI T GA V A H GP P P Sbjct: 771 EGITGFTQGAHHVHAADHQTLGPQGRPAEFP 801 >gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 913 Score = 128 bits (321), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 108/333 (32%), Positives = 170/333 (51%), Gaps = 28/333 (8%) Query: 26 NSFVLGWLXNVIRTQSNNKLRMEDWKGQEHIKLSTEHSGKSQLNLGHLVD----SQRKER 81 N F+ G I+ Q N+LR++D Q +L+++H G+S+LNLG L + R Sbjct: 547 NRFLAGIKSKEIQGQRYNQLRLDDTPQQISAQLASQH-GQSELNLGWLTHPRAGGRSDAR 605 Query: 82 GQGVELRTDAHAAIRGGKGVFINADAQPGAQGKQLDMQAAQTLLEQALQQTQSLAESANA 141 G+G ELRTD A+RGG+GV I+A + A G QLD LL LQ Q + A Sbjct: 606 GEGAELRTDQALALRGGQGVLISAVMRQEASGTQLDRAEMLGLL-HVLQDVQQ--QLATL 662 Query: 142 AQALAADCEQQRAL--LNDTLLSLK-------------RAGILASAPAGIALTSGSHLQL 186 AQ AD Q + L D L + + + + +APAG+A+TS + L Sbjct: 663 AQTHHADDTQLQTFSRLRDQLDAWEAGSNTQAKSDGGGKPMVAVTAPAGVAITSDEGVAL 722 Query: 187 AAQEHFIATAGGNADISVMKRFTAAAGEAVSLFAQKLGMKLFAAKGKVEIQAQSDDINII 246 AQ+ + GN S K+ A E++ LFAQ+LG++L AA GK+++Q D+ + Sbjct: 723 GAQKEIDLISVGNTQFSAGKKLLARVAESICLFAQRLGIQLVAANGKLQLQTHGGDVEVT 782 Query: 247 SDRNMFITSANGRVTVEAKDELLLKCGGSYIRINASGIEDGTPGASVVKAGS-HGRPGPA 305 S R + +T+A+ + ++A L L+ G+ I + I + G V+++G+ H G Sbjct: 783 SARRIVLTAAD-EIVLQAP-RLRLQAQGAQIDLGGGVITQQSSGDHVIRSGNFHHVSGAD 840 Query: 306 SLPPALPQLPKSVCKECLKEALRARAKTSVPNI 338 S PP + +LP S + + L AR + P++ Sbjct: 841 SQPPGV-ELPASTMRTDERFIL-ARRGSGRPHV 871 >gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 954 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 103/352 (29%), Positives = 168/352 (47%), Gaps = 46/352 (13%) Query: 29 VLGWLXNVIRTQSNNKLRMEDWKGQEHIKLSTEHSGKSQLNLGHLVD-----SQR-KERG 82 + GW N+ ++D + Q ++L++ + KSQLNLGHLV+ +QR RG Sbjct: 555 ISGWHSTAHDGSGFNQWVVDDTQSQLRMRLASS-TAKSQLNLGHLVEQADTGAQRGSYRG 613 Query: 83 QGVELRTDAHAAIRGGKGVFINADAQP----GAQGKQLDMQAAQTLLEQALQQTQSLAES 138 QG ELR+DA +RGG+G+ ++ A+P G Q+D A L+ A Q++++S Sbjct: 614 QGFELRSDAWGVVRGGEGLLLSTTARPLNGASVTGTQMDAAGAIAQLKGAKSLAQTMSDS 673 Query: 139 ANAAQALA------------------------ADCEQQRALLND----------TLLSLK 164 A QAL+ A Q AL +D + Sbjct: 674 ATQQQALSSTQALQAKTDFLALIDPSDQGKHPASVNGQDALKSDGSTRNTDAAQPVEKFA 733 Query: 165 RAGILASAPAGIALTSGSHLQLAAQEHFIATAGGNADISVMKRFTAAAGEAVSLFAQKLG 224 +A +L +P+ I TS + L A E+ T G+ S +AAAG+A S ++ G Sbjct: 734 KAAVLMDSPSSINWTSNASTLLYAAENLQWTTQGDVHWSAADTASAAAGKAASFYSHDGG 793 Query: 225 MKLFAAKGKVEIQAQSDDINIISDRNMFITSANGRVTVEAKDELLLKCGGSYIRINASGI 284 ++ FAA G V +QA +D + I++D+++ +TS N + ++A+ +++L+ G + + + I Sbjct: 794 IQAFAANGPVSLQAHTDALEILADKDVTVTSVNDSIEIKARQKIVLQAGQASVTLEGGNI 853 Query: 285 EDGTPGASVVKAGSHGRPGPASLPPALPQLPKSVCKECLKEALRARAKTSVP 336 PG VK +H PG AS+ P L LP + K EA + P Sbjct: 854 TFACPGTFSVKGATHAFPGGASVSPDLMALPDTKYK-LFDEAFVVKDPNGTP 904 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 863,304 Number of extensions: 30758 Number of successful extensions: 171 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 9 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)