BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0052.0002.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 224 7e-60 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 70 2e-13 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 224 bits (570), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 107/133 (80%), Positives = 118/133 (88%), Gaps = 3/133 (2%) Query: 6 IDRTPEAYGYPLLIKQLLHSSMATAPAQEIVYGIDNQRRHTYADLNVRIQRLGNMLRELG 65 I +TP AYGYPLLIKQLLHS++ATA QEI+YG QRR +YA R+QRL N L+E+G Sbjct: 5 IHQTPSAYGYPLLIKQLLHSALATASGQEIIYG---QRRLSYAGFYERVQRLANALKEMG 61 Query: 66 LQPGNTVAVMDWDSHRYLECFFAVPMLGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSD 125 LQPGNTVAVMDWDS+RYLECFFAVPM+GCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSD Sbjct: 62 LQPGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSD 121 Query: 126 FLPVLKQIRGELN 138 FLP+LKQIRGEL Sbjct: 122 FLPLLKQIRGELT 134 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 44/136 (32%), Positives = 69/136 (50%), Gaps = 12/136 (8%) Query: 36 VYG-----IDNQRRHTYADLNVRIQRLGNMLRELGLQPGNTVAVMDWDSHRYLECFFAVP 90 VYG I R + R +RL + L+ LG+ +TV+ M ++ +E F VP Sbjct: 30 VYGKRTAIIHGALRQDWDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVP 89 Query: 91 MLGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSDFLPVLKQIRGELNINILMELFMPSR 150 M G VL +N+RL E I + L H +A VLL++S+F + +Q+R +L ++E+F Sbjct: 90 MAGAVLNALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIVEVFDELG 149 Query: 151 PGAGASARPPASRRRL 166 P PP + R Sbjct: 150 P-------PPVAGERF 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 781,234 Number of extensions: 29318 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 85 Number of HSP's successfully gapped: 2 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)