BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0016.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 367 e-103 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 246 2e-66 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 191 9e-50 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 157 9e-40 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 150 1e-37 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 367 bits (942), Expect = e-103, Method: Compositional matrix adjust. Identities = 181/286 (63%), Positives = 233/286 (81%) Query: 1 VAGIGILRLKAVTEETRSMMDEPLRSERLVSEWNTLLLNAIQRTTSVVKSKDPELDAFLS 60 VA +GI RLK V +T+ MMD PL++ERLVS+WNTLLL AIQRTT+VVKS DPEL+AFL+ Sbjct: 25 VAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTLLLIAIQRTTTVVKSHDPELEAFLA 84 Query: 61 KEAAQSSKASAETLAQVEKLLSTEEERQLFKNINDYRKKFLTVRDSIYKLKKDGKAEEIA 120 KEAA SSK SA+TL VE+LL++EEE+++F IN RK+FLT+RD IY KK G A ++ Sbjct: 85 KEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVARKQFLTIRDQIYAAKKQGDAAQVE 144 Query: 121 AVFEAEYLPVAKGTQDAMRKLLDFERGRIDTIAQHVETTAENSQKLIFALEGVILLAGIF 180 +++ +Y+ +AK TQDAMR LLDFER RID I+ ++ A +SQ I LE VILL GI Sbjct: 145 ELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQIQQDASSSQHQIMLLEVVILLCGIA 204 Query: 181 FAIVLTKSITRPIDMALAISKQVSSGDLTSEARIVSKDEVGQLVMSLQSMSEQLHVIVSN 240 FA++LT+SITRP+ +ALAIS++V++GDLTS A+ + +DE+GQLV SLQ MSE+LH +VS Sbjct: 205 FAVLLTRSITRPVHVALAISQRVAAGDLTSRAQAMGRDELGQLVASLQGMSERLHGVVST 264 Query: 241 VRNGADSIAIASGEIASGNLDLSSRTEEQAGALEETAASIEQXAAS 286 VR+GADSIA+ASGEIA+GNLDLS+RTEEQAGALE+TAASIE+ +S Sbjct: 265 VRHGADSIAMASGEIANGNLDLSARTEEQAGALEQTAASIEELTSS 310 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 246 bits (628), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 139/282 (49%), Positives = 194/282 (68%) Query: 1 VAGIGILRLKAVTEETRSMMDEPLRSERLVSEWNTLLLNAIQRTTSVVKSKDPELDAFLS 60 +AGIG+ RL V + TR MM EPL++ERL+++W T L+ I+RT ++ KS DP L + + Sbjct: 27 IAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNLVAGIRRTIAIAKSSDPALGPYFA 86 Query: 61 KEAAQSSKASAETLAQVEKLLSTEEERQLFKNINDYRKKFLTVRDSIYKLKKDGKAEEIA 120 E A SSK+S E QVE LLST EE+ LF+ I + RK +L+ RD I K K G EE Sbjct: 87 AEVAASSKSSGEYQKQVEALLSTPEEKALFQKIGELRKLYLSSRDEINKAKAAGNTEEAM 146 Query: 121 AVFEAEYLPVAKGTQDAMRKLLDFERGRIDTIAQHVETTAENSQKLIFALEGVILLAGIF 180 + + ++P A QD MRKL+DF+R +D A+ ++ A S+ LI LEG+ILL GI Sbjct: 147 RILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAREIDAIAAKSRTLILVLEGLILLLGIV 206 Query: 181 FAIVLTKSITRPIDMALAISKQVSSGDLTSEARIVSKDEVGQLVMSLQSMSEQLHVIVSN 240 FA LT IT+P+ A+ +S+ V+ GDL+SE + S+DE GQL+ SL+ M++ L IVSN Sbjct: 207 FARYLTLGITKPLHRAVDVSRCVAEGDLSSEIAVTSRDETGQLLQSLKDMNQSLRGIVSN 266 Query: 241 VRNGADSIAIASGEIASGNLDLSSRTEEQAGALEETAASIEQ 282 VR+G D+I+ AS EIA+GNLDLSSRTE+QA +L ETA+++E+ Sbjct: 267 VRSGTDTISTASSEIAAGNLDLSSRTEQQASSLAETASAMEE 308 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 191 bits (484), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 115/285 (40%), Positives = 181/285 (63%), Gaps = 3/285 (1%) Query: 1 VAGIGILRLKAVTEETRSMMDEPLRSERLVSEWNTLLLNAIQRTTSVVKSKDPELDAFLS 60 V + I RL+ + T MM EPL ERLVS+W + +++RTT++ KS DP L AF + Sbjct: 27 VVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTIHTSVRRTTAIAKSSDPSLAAFFA 86 Query: 61 KEAAQSSKASAETLAQVEKLLSTEEERQLFKNINDYRKKFLTVRDSIYKLKKDGKAEEIA 120 +AA +S+ S E +E L+ +++E+ +F + + RK ++ RD+I K K +G+ EE A Sbjct: 87 DDAAAASRMSTEQQKAIEALIESDQEKAVFTKLGEVRKDYIKYRDAISKAKAEGRTEEAA 146 Query: 121 AVFEAEYLPVAKGTQDAMRKLLDFERGRIDTIAQHVETTAENSQKLIFALEGVILLAGIF 180 + + KG D +++LL +R ID +A H++T S+ L+ AL + ++ G Sbjct: 147 QILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAAHIQTVYRESRNLMLALAFLQVVLGWL 206 Query: 181 FAIVLTKSITRPIDMALAISKQVSSGDLTS--EARIVSK-DEVGQLVMSLQSMSEQLHVI 237 FA L ITRP++ A+ +++ V++GDLT + R+ ++ DE G+L+ +L +M+ L I Sbjct: 207 FARTLALGITRPLEQAVEVAEAVAAGDLTHRIDERLAARGDETGKLMHALHAMTGNLARI 266 Query: 238 VSNVRNGADSIAIASGEIASGNLDLSSRTEEQAGALEETAASIEQ 282 V VR G D+IA AS EIA+GNLDLS+RTE QAGALEETA+++E+ Sbjct: 267 VGQVRGGTDAIATASKEIATGNLDLSARTERQAGALEETASAMEE 311 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 157 bits (397), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 96/280 (34%), Positives = 161/280 (57%), Gaps = 2/280 (0%) Query: 4 IGILRLKAVTEETRSMMDEPLRSERLVSEWNTLLLNAIQRTTSVVKSKDPELDAFLSKEA 63 +G+ RL ++ + T M L+ ER +W+ + RT +V+KS D A+ K+ Sbjct: 27 LGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKENGVRTFAVMKSDDDAFQAYFQKQI 86 Query: 64 -AQSSKASAETLAQVEKLLSTEEERQLFKNINDYRKKFLTVRDSIYKLKKDGKAEEIAAV 122 AQS++ S + +VE ++ EE++LF + R + R SI+ +K G + Sbjct: 87 DAQSARIS-QLQKEVEAAITDAEEKRLFDEVGKLRAAYRATRQSIFDVKTAGDDARAREM 145 Query: 123 FEAEYLPVAKGTQDAMRKLLDFERGRIDTIAQHVETTAENSQKLIFALEGVILLAGIFFA 182 + + + D + + DF++ ID A + + + ++ AL + ++ G A Sbjct: 146 TDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRDYRSGRSMLLALGAIEIVLGAALA 205 Query: 183 IVLTKSITRPIDMALAISKQVSSGDLTSEARIVSKDEVGQLVMSLQSMSEQLHVIVSNVR 242 +LT+SITRP++ A+ I++ V++GDLTS S+DE G+L+ +L++M+ L IV VR Sbjct: 206 WLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGRVR 265 Query: 243 NGADSIAIASGEIASGNLDLSSRTEEQAGALEETAASIEQ 282 G ++I AS EIA+GNLDLS+RTE QAG+LEETA+++EQ Sbjct: 266 MGTETINTASREIATGNLDLSARTEAQAGSLEETASAMEQ 305 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 150 bits (379), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 92/229 (40%), Positives = 146/229 (63%), Gaps = 9/229 (3%) Query: 58 FLSKEAAQSSKASAETLAQVEKLLSTEEERQLFKNINDYRKKFLTVRDSIYKLKKDGKAE 117 +L + AA S+K + E L ++EK+LST R+LF++I + R + T RD + +L K K E Sbjct: 84 YLDRSAANSAKTT-EALGKMEKILSTARGRELFQSIQEARSAYNTPRDKLRELIKQQKKE 142 Query: 118 EIAAVFEAEYLPVAKGTQDAMRKLLD----FERGRIDTIAQHVETTAENSQKLIFALEGV 173 E + + +P QD ++LD F++ ++ T +++ ++ L V Sbjct: 143 EAVELLFTQVIPA----QDKYFQVLDQFVAFQKSLMEQSVTEGRDTTQSAIAMMLGLSAV 198 Query: 174 ILLAGIFFAIVLTKSITRPIDMALAISKQVSSGDLTSEARIVSKDEVGQLVMSLQSMSEQ 233 + + A ++T+SITRP++ A++++ V++GDLT + SKDE G L+ +L+SM++ Sbjct: 199 AIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGATSKDETGALLAALKSMNDS 258 Query: 234 LHVIVSNVRNGADSIAIASGEIASGNLDLSSRTEEQAGALEETAASIEQ 282 LH IVS VR G+D+I AS EIA+GNLDLSSRTEEQAGALEETA+++E+ Sbjct: 259 LHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEETASAMEE 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.332 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 708,099 Number of extensions: 24149 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 61 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 61 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)