BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0015.p (837 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313659|ref|YP_003777751.1| amidase family protein [Herbasp... 744 0.0 gi|300313661|ref|YP_003777753.1| glycine/D-amino acid oxidase [H... 476 e-135 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 198 2e-51 gi|300312309|ref|YP_003776401.1| 6-aminohexanoate-cyclic-dimer h... 126 7e-30 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 120 5e-28 >gi|300313659|ref|YP_003777751.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 506 Score = 744 bits (1921), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/505 (75%), Positives = 409/505 (80%), Gaps = 46/505 (9%) Query: 5 SIVEKSAVALRALIGARQLSPVELLDACISRIEDINPRVNAVTATCFERARDEARRAEQA 64 +VEKSAV LR LIG++QLSPVELL+ACI+RIEDINP +NAVTATCFERAR EAR AEQA Sbjct: 4 KLVEKSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAAEQA 63 Query: 65 VMAGAPLGLLHGLPIGIKDLEETEGLLTTYGSAIYRSNIPARDNVLVARLRRAGAIVAGK 124 V+ G PLGLLHGLPIGIKDLEETEGLLTTYGS +YR NIPARDN LVARLR AGAIVAGK Sbjct: 64 VIDGKPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVAGK 123 Query: 125 TNVPEMGAGANSRNTVWGATGNPFNPLLNAGGSSGGSAAALACDLLPLCSGSDTGGSLRI 184 TNVPEMGAGANSRN VWGATGNPFNPLLNAGGSSGGSAAALA DLLPLCSGSDTGGSLRI Sbjct: 124 TNVPEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSLRI 183 Query: 185 PAAKCGVVGFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRASAGMDSSDPLSY 244 PAAKCGVVGFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRA+ G+ +SDPL Y Sbjct: 184 PAAKCGVVGFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDPLGY 243 Query: 245 PLGDDPFATLPDIDLSQLRIGYTEDFGVCDVDNDIRAVSRLTQHSILVEHYCVHAVFLVS 304 + D FA+LP +DLSQLRIGYTEDFGVCDVDN IR V R Sbjct: 244 AVSDSEFASLPQVDLSQLRIGYTEDFGVCDVDNGIRQVFR-------------------- 283 Query: 305 HSPGHSLLTDRHAQKTERMKTCPQVXIKIAAIRPHVKTCEQIDVDMGEAHRCFDVIRAES 364 K+AAI P+VK CE +DVDMGEAHR FDVIRAE+ Sbjct: 284 --------------------------DKMAAIAPYVKLCEPVDVDMGEAHRAFDVIRAEA 317 Query: 365 FFTGFDAAYRRDPSSLGPNTRANYELGAAMKLADTAWAHSEQTRIFRRFQAQFSRFDLIL 424 F GFDAAYRRDP+SLGPNTRANYE+GAAM L D AWA SEQTRIFRRFQ + R+DLIL Sbjct: 318 FVAGFDAAYRRDPASLGPNTRANYEMGAAMSLLDCAWAQSEQTRIFRRFQQLYERYDLIL 377 Query: 425 SPTTPVSPFPWSQLYLKEINGVELENYYRWLALTYVVTLATNPSISLPCGTDHRQMPFGL 484 SPTTPVSPFPWS+LYLKE+NGV LENYYRWLALTYVVTLATNP+ISLPCG DHR MPFGL Sbjct: 378 SPTTPVSPFPWSELYLKEVNGVALENYYRWLALTYVVTLATNPAISLPCGRDHRGMPFGL 437 Query: 485 QVTGAFRADRHLLGCAAALENLFDG 509 QVTG FR D +L CAAALE F G Sbjct: 438 QVTGPFRGDARVLACAAALEQAFAG 462 >gi|300313661|ref|YP_003777753.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 379 Score = 476 bits (1224), Expect = e-135, Method: Compositional matrix adjust. Identities = 245/315 (77%), Positives = 258/315 (81%), Gaps = 4/315 (1%) Query: 511 IAGASIAYWLAPHAGVIMLERESQPGYHSTGRSAAAYMESYGPPQVRALTCASRAFFDHP 570 IAGASIAYWLA H V+++ERESQPGYHSTGRSAA YMESYGPPQVRALTCASRAFFDHP Sbjct: 16 IAGASIAYWLARHGSVLLVERESQPGYHSTGRSAALYMESYGPPQVRALTCASRAFFDHP 75 Query: 571 PAGFAEHPLLTPRGALFIARTDQLDQLDAHEALVRSVSDKVQRLSPDEACARVPVLRREA 630 P GFAEHPLLTPRGALF A + Q QLD HEALVRSVSDKVQRL + A VPVLR E Sbjct: 76 PEGFAEHPLLTPRGALFFANSAQRAQLDEHEALVRSVSDKVQRLDAQQTRAMVPVLRDED 135 Query: 631 LGGAVYEEDACDIDVHGLHQGFLRGARGAGARVVCDAEVLGLAY-LDGQ-WRLSTSQGEF 688 L GAVYEEDA DIDVH LHQGFLRGAR AGARV+C AEV + D Q WR+ TSQGE Sbjct: 136 LIGAVYEEDASDIDVHALHQGFLRGARAAGARVLCHAEVQAITRDEDSQTWRVHTSQGEL 195 Query: 689 RAPVVINAAGAWADVIGGMAGARPIGLVPKRRSAFTFTADAGQDSTSWPMFMSVDESFYI 748 V++NAAGAWAD IG +AGA PIGL PKRRSAFTF A AG S WPMFM VDESFYI Sbjct: 196 LGGVIVNAAGAWADEIGRLAGAAPIGLQPKRRSAFTFAAPAG--SEHWPMFMPVDESFYI 253 Query: 749 KPDAGLLLGSPANADPVEPHDVQAEELDIAIAIDQIQNATTLAIRRPSHVWAGLRSFVGD 808 KPDAGLLLGSPANADPV P DVQAEELDIAIAID IQNATTL IRRP+HVWAGLRSFV D Sbjct: 254 KPDAGLLLGSPANADPVPPQDVQAEELDIAIAIDHIQNATTLEIRRPAHVWAGLRSFVAD 313 Query: 809 GSFVGGFDSGLSGFL 823 GSFVGG+D L GF Sbjct: 314 GSFVGGYDRELPGFF 328 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 198 bits (503), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 168/509 (33%), Positives = 237/509 (46%), Gaps = 51/509 (10%) Query: 4 HSIVEKSAVALRALIGARQLSPVELLDACISRIEDINPRVNAVTAT-CFERARDEARRAE 62 H +V A+AL A I ++++S E++ A + IE +NP VNA+ + E DEA+RA+ Sbjct: 2 HQLVTLDALALSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRAD 61 Query: 63 QAVMAGAPLGLLHGLPIGIKDLEETEGLLTTYGSAIYRSNIPARDNVLVARLRRAGAIVA 122 Q + G LG +HG+P KDL +GL T+ GS + D VAR+R AGAI Sbjct: 62 QLLAQGRYLGWMHGMPQAPKDLASCKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIFI 121 Query: 123 GKTNVPEMGAGANSRNTVWGATGNPFNPLLNAGGSSGGSAAALACDLLPLCSGSDTGGSL 182 GKTNV E G G+NS N ++G T N ++ AGGSSGG+ AALA +LP+ GSD GSL Sbjct: 122 GKTNVSEFGLGSNSYNPLFGVTRNAYDFSRIAGGSSGGAGAALAMRMLPVADGSDMMGSL 181 Query: 183 RIPAAKCGVVGFRPSPGLVP-SERKLLGWTPISVVGPMGRDVADTVLQLRASAGMDSSDP 241 R PAA V GFRP+ G VP L +S GPMGR VAD AG D P Sbjct: 182 RNPAAFNNVYGFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQAGYDPQCP 241 Query: 242 LSYPLGDDPFATLPDIDLSQLRIGYTEDF-GVCDVDNDIRAVSRLTQHSILVEHYCVHAV 300 LS A D RIG+ ++ G ++ ++ Sbjct: 242 LSSDTDVTGDAAQLKRDFKGARIGWLGNYDGYLPIEEEV--------------------- 280 Query: 301 FLVSHSPGHSLLTDRHAQKTERMKTCPQVXIKIAAIRPHVKTCEQIDVDMGEAHRCFDVI 360 M C A I V+ C D M + + V Sbjct: 281 ----------------------MVLCEAALSDFADIGCTVEQCRP-DYPMEKLWETWLVH 317 Query: 361 RAESFFTGFDAAYRRDPSSLG---PNTRANYELGAAMKLADTAWAHSEQTRIFRRFQAQF 417 R + G + Y DP+ G P + E G + AD A ++ +R F Sbjct: 318 R-QWLVGGNLSVYFADPARRGLLKPEAQWEVESGQYLTAADVFRASMARSDWYRAIHKLF 376 Query: 418 SRFDLILSPTTPVSPFPWSQLYLKEINGVELENYYRWLALTYVVTLATNPSISLPCGTDH 477 +FD +L P+ + PF + + + + G E++ Y+RW+ + TLA P++S+P G Sbjct: 377 EQFDYLLLPSAQMFPFDVATKWPRNVAGREMDTYHRWMEVVIGPTLAGLPAMSVPVGFGA 436 Query: 478 RQMPFGLQVTGAFRADRHLLGCAAALENL 506 +P G+Q+ G R DR +L A ALE L Sbjct: 437 NGLPMGMQIIGPSRDDRGVLQLAYALEGL 465 >gi|300312309|ref|YP_003776401.1| 6-aminohexanoate-cyclic-dimer hydrolase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 126 bits (317), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 87/230 (37%), Positives = 121/230 (52%), Gaps = 9/230 (3%) Query: 18 IGARQLSPVELLDACISRIEDINPRVNAVTATCFERARDEARRAEQAVMAGAPLGLLHGL 77 IG +LSP E L I R+E +NP +NAV + EQ+V G P G G+ Sbjct: 22 IGQGELSPGEALAQAIERLEQVNPALNAVAEKLYHLG-------EQSVAQGLPDGPFRGV 74 Query: 78 PIGIKDL-EETEGLLTTYGSAIYRSNIPARDNVLVARLRRAGAIVAGKTNVPEMGAGANS 136 P+ KDL G + GS + + N+ D LV RL++AG + G T PE G + Sbjct: 75 PLLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIFGTTTSPEFGTSYTT 134 Query: 137 RNTVWGATGNPFNPLLNAGGSSGGSAAALACDLLPLCSGSDTGGSLRIPAAKCGVVGFRP 196 + ++GAT NP++ + GGSSG +AA +A +LP+ G+D GGSLR+PA+ CGV G +P Sbjct: 135 ESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSLRVPASACGVFGLKP 194 Query: 197 SPGLVP-SERKLLGWTPISVVGPMGRDVADTVLQLRASAGMDSSDPLSYP 245 S GL+P GW +S M V D+ L AG D P + P Sbjct: 195 SRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAPYAAP 244 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 120 bits (301), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 150/526 (28%), Positives = 227/526 (43%), Gaps = 95/526 (18%) Query: 6 IVEKSAVALRALIGARQLSPVELLDACISRIE---DINPRVNAVTATCF-ERARDEARRA 61 +V+ + + +R + R+ S +E +DA I+ E D+N C+ +R ++ R Sbjct: 3 LVDMTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLN---------CYLQRDPEQLRSQ 53 Query: 62 EQAVMAGAPLGLLHGLPIGIKDLEETEGLLTTYGSAIYRSNIPARDNVLVARLRRAGAIV 121 + + G L G+PI IKD + G+ +T G+A R+N+P R +V L AGA+ Sbjct: 54 ARMLDEQGGHGPLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALH 113 Query: 122 AGKTNVPEMGAGANSRNTVWGATGNPFNPLLNAGGSSGGSAAALACDLLPLCSGSDTGGS 181 AGK N+ E+ G + N V+G NP++ L AGGSSGG A LA L P G+DTGGS Sbjct: 114 AGKANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGS 173 Query: 182 LRIPAAKCGVVGFRPSPGLVPSER-KLLGWTPIS----VVGPMGRDVADTVLQLRASAGM 236 +RIPAA CGVVG RP+ S R G PIS GPM R VAD L + AG Sbjct: 174 VRIPAALCGVVGLRPT-----SRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGS 228 Query: 237 DSSDPLSYPLGDDPFATLPDIDLSQLRIGYTEDFGVCDVDNDIR-----AVSRLTQHSIL 291 S PL A P SQLR+G + ++ ++ A+ +L + + Sbjct: 229 RDSAPL--------LALPP----SQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVE 276 Query: 292 VEHYCVHAVFLVSHSPG-----HSLLTDRHA--QKTERMKTCPQVXIKIAAIRPHVKTCE 344 + + + +SH+ G H L D A Q ++ +V ++ + P V Sbjct: 277 LVDVDLPTLAPLSHAAGFAIVLHEFLIDLPAYLQASDARDVIKRVLAEVGS--PDVARII 334 Query: 345 QIDVDMGEAHRCFDVIRAESFFTGFDAAYRRDPSSLGPNTRANYELGAAMKLADTAWAHS 404 Q +D P Y L Sbjct: 335 QATLDQ-------------------------------PVPEHAYRLAL------------ 351 Query: 405 EQTRIFRRFQAQFSR---FDLILSPTTPVSPFPWSQLYLKEINGVELENYYRWLALTYVV 461 EQ R + R D ++ PTT ++ P + + G + + + Sbjct: 352 EQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGA 411 Query: 462 TLATNPSISLPCGTDHRQMPFGLQVTGAFRADRHLLGCAAALENLF 507 ++A PSIS+P G +P GL++ G +DR LL AA+LE + Sbjct: 412 SIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLETVL 457 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,509,374 Number of extensions: 104632 Number of successful extensions: 314 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 14 Length of query: 837 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 744 Effective length of database: 1,179,515 Effective search space: 877559160 Effective search space used: 877559160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)