BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0014.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 573 e-164 gi|300309906|ref|YP_003773998.1| major facilitator superfamily p... 248 5e-67 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 127 2e-30 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 119 5e-28 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 100 2e-22 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 573 bits (1476), Expect = e-164, Method: Compositional matrix adjust. Identities = 279/358 (77%), Positives = 320/358 (89%), Gaps = 6/358 (1%) Query: 1 LLSSFFLGYSLLQLPGGWLADRFGSRPVIVISILMWSVFTAFTGLAWSISSLVIIRFVFG 60 LLSSFFLGYSLLQLPGGWLADR+GSRPVIVISIL+WS+FT TG+AWS++SLV+IRF+FG Sbjct: 53 LLSSFFLGYSLLQLPGGWLADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFG 112 Query: 61 LGEGAFPAASVKGVAETFSKEERPKMSSLLMSSNYVGSMLAPLIIAPMIIQFGWRNVFHY 120 +GEGAFPAASVKGVAE FS++ERPKMSSLLMSSNY+GSMLAPL+IAP+I+ +GWR VFHY Sbjct: 113 VGEGAFPAASVKGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHY 172 Query: 121 IGIAGLAFALMYWFVVKPVRAARGDAAAEAEKKAINKQAFRTLLKMPLMWQIVAVWFGLS 180 IGIAG+ FAL+YWF V+PVR G A A INKQAF L+KMPLMWQIVAVWFGLS Sbjct: 173 IGIAGVVFALVYWFCVRPVRPGVGGAGA------INKQAFMALMKMPLMWQIVAVWFGLS 226 Query: 181 IVNKGLDSWMPTYLMTQRGLNLKSVGLLLPLPYVLAGIATGIGGWVMMRFFDGRERYLLI 240 I+NKGLD+WMPTYLMT RGLNLK+VGLLLPLPY++AG++T IGGWVM+RFFDGRE+ LLI Sbjct: 227 IINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVRFFDGREKLLLI 286 Query: 241 GSSILTAIFVYCMYTSNSVAALITYQCIAYFFKSFVLATCIALPTKMLPANLIGTSIGMV 300 GSS+LTA+FVYCMY++ SV +I +QC+AYFFKSFVLATCIALPTKML A L+G+ +GMV Sbjct: 287 GSSLLTAVFVYCMYSAASVEGVIAWQCLAYFFKSFVLATCIALPTKMLQAQLVGSGVGMV 346 Query: 301 NLGGQSAGFISPLVIGFLVSAFSNYDYAFSFLIAAACFSVLVSLFIRTLKSSAYAQHA 358 NLGGQ AGFISPLVIGFLVSAF+NYDYAFSFLI AA FSVLVSLFIRT KS + Q A Sbjct: 347 NLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAAFFSVLVSLFIRTHKSDSQMQPA 404 >gi|300309906|ref|YP_003773998.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 248 bits (633), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 132/348 (37%), Positives = 219/348 (62%), Gaps = 6/348 (1%) Query: 1 LLSSFFLGYSLLQLPGGWLADRFGSRPVIVISILMWSVFTAFTGLAWSISSLVIIRFVFG 60 +LS+F++ Y+L+QL GGWL+DR+GSR V+V+ ++ WS+FT T +AWS +SL++IRF+FG Sbjct: 52 VLSAFYVSYALMQLMGGWLSDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFG 111 Query: 61 LGEGAFPAASVKGVAETFSKEERPKMSSLLMSSNYVGSMLAPLIIAPMIIQFGWRNVFHY 120 GEG F AS VAE F ++ER + S L+S+ ++G+ + ++A ++ GWR FH Sbjct: 112 AGEGCFSPASSVTVAEVFPQQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHI 171 Query: 121 IGIAGLAFALMYWFVVKPVRAARGDAAAEAEKKAINKQAFRTLLKMPLMWQIVAVWFGLS 180 + G+ A + W ++ G AA +A A K R L+K P ++ A+WF S Sbjct: 172 LAFVGMIVAAILWLALR-----SGMAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTS 226 Query: 181 IVNKGLDSWMPTYLMTQRGLNLKSVGLLLPLPYVLAGIATGIGGWVMMRFFDGRERYLLI 240 IV G+ SWMP++L+ ++L +G+ +PY+LA + T + GW++ + GRE+Y ++ Sbjct: 227 IVYIGMISWMPSFLLKVYQIDLLHIGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYFMM 286 Query: 241 GSSILTAIFVYCMYTSNSVAALITYQCIAYFFKSFVLATCIALPTKMLPANLIGTSIGMV 300 G ++ A+F+ M ++ + L+ Y + +FV AT ++P K P++LIG++ G++ Sbjct: 287 GGALACAVFLALMISTTVIELLVVYWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLM 346 Query: 301 NLGGQSAGFISPLVIGFLVSAFSN-YDYAFSFLIAAACFSVLVSLFIR 347 N GGQ AG I+P+V+G L+ AF+ Y AF FL+ +A S L+++ R Sbjct: 347 NFGGQLAGSIAPVVMGSLIVAFNGAYLAAFWFLVGSAMMSFLIAMTWR 394 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 103/377 (27%), Positives = 185/377 (49%), Gaps = 34/377 (9%) Query: 1 LLSSFFLGYSLLQLPGGWLADRFGSRPVIVISILMWSVFTAF-TGLAW-----SISSLVI 54 + S+F Y L QLPGGWL DRFGS+ + + SI +WSVFT G+ W +++SL + Sbjct: 54 IFSAFSWAYVLAQLPGGWLLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFL 113 Query: 55 IRFVFGLGEG-AFPAASVKGVAETFSKEERPKMSSLLMSSNYVGSMLAPLIIAPMIIQFG 113 +RF+ GL E +FPA + VA F +ER S++ S+ Y ++L ++A ++ + Sbjct: 114 LRFMVGLAEAPSFPANG-RIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYS 172 Query: 114 WRNVFHYIGIAGLAFALMYWFVV-KPVRAARGDAAAEAEKKA-------INKQA------ 159 W +VF +G+ G+A AL++ ++ P R + A A ++ N++ Sbjct: 173 WHHVFTVMGVVGIALALIWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTK 232 Query: 160 ----FRTLLKMPLMWQIVAVWFGLSIVNKGLDSWMPTYLMTQRGLNLKSVGLLLPLPYVL 215 R LL ++ + + ++++ +W P YL+ +RG+++ G + +P V Sbjct: 233 GRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVC 292 Query: 216 AGIATGIGGWVMMRFFDG------RERYLLIGSSILTAIFVYCMYTSNSVAALITYQCIA 269 I +GG + R + ++G +L+ + C Y + ++ +A Sbjct: 293 GFIGGVVGGLISDRLIKRGLSVTWARKIPIVGGMLLSMTMILCNYV-EAQWMVVGIMALA 351 Query: 270 YFFKSFVLATCIALPTKMLPANLIGTSIGMVNLGGQSAGFISPLVIGFLVSAFSNYDYAF 329 +F K V A A+ P ++G S G+ N+ G AG +P+VIG++V ++ A Sbjct: 352 FFGKG-VGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGAL 410 Query: 330 SFLIAAACFSVLVSLFI 346 F+ A +V+ L I Sbjct: 411 VFVGINALVTVISYLVI 427 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 119 bits (297), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 97/379 (25%), Positives = 174/379 (45%), Gaps = 36/379 (9%) Query: 1 LLSSFFLGYSLLQLPGGWLADRFGSRPVIVISILMWSVFTAFTGLAWSISSLVIIRFVFG 60 L S F YSL QLP G RFG V +++L SVFT G A S+ +L I RF G Sbjct: 58 LASIFLFAYSLAQLPSGLAYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFGLG 117 Query: 61 LGEGAFPAASVKGVAETFSKEERPKMSSLLMSSNYVGSMLAPLIIAPMIIQFGWRNVFHY 120 L EG P + F E+ M+ + +++ G +L P + A ++ +GWR++F++ Sbjct: 118 LAEGPLPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYF 177 Query: 121 IGIAGLAFALMYWFVVK---------------PVRAARGDAAAEAEKKAINKQ------- 158 + G+ ++++ VVK + ++G AA + A+ + Sbjct: 178 FAVPGVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRI 237 Query: 159 -------AFRTLLKMPLMWQIVAVWFG----LSIVNKGLDSWMPTYLMTQRGLNLKSVGL 207 T + W I+ G +IVN L +W+PTYL++ +G ++ ++G+ Sbjct: 238 IRRSKVATIETTKGVFCSWNIMGAALGYFFMTAIVNV-LLAWIPTYLISVKGYSIANMGM 296 Query: 208 LLPLPYVLAGIATGIGGWVMMRFFDGRERYLLIGSSILTAIFVYCMYTSNSVAALITYQC 267 + P+V A I +GG + R+ + R + ++ S+ T I + + S + L Sbjct: 297 VAAAPWVGAVIGNLLGGAMTDRWLEKRRKPAMMMSAFFTTIMMVILINSPADPLLYGALL 356 Query: 268 IAYFFKSFV-LATCIALPTKMLPANLIGTSIGMVNLGGQSAGFISPLVIGFLVSAFS-NY 325 + + +T + P M + + MVN GQ G +PL GFL+ ++S N+ Sbjct: 357 LLTGILLNIGYSTYMVYPMGMTSKKVYPMACAMVNTLGQLGGACAPLAAGFLLDSYSWNH 416 Query: 326 DYAFSFLIAAACFSVLVSL 344 + F + + CF ++++ Sbjct: 417 VWIFMAVCSILCFITILTI 435 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 94/366 (25%), Positives = 173/366 (47%), Gaps = 23/366 (6%) Query: 1 LLSSFFLGYSLLQLPGGWLADRFGSRPVIVISILMWSVFTAFTGLAWSISSLVIIRFVFG 60 LLS+F L Y+ QLP G + D+FG+R ++ + +WSV G ++ ++ R G Sbjct: 57 LLSAFSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLG 116 Query: 61 LGEG-AFPAASVKGVAETFSKEERPKMSSLLMSSNYVGSMLAPLIIAPMIIQFGWRNVFH 119 LGE FPA + K V+E F+ ER + + ++S+ +G LAP I+ +++ FGWR +F Sbjct: 117 LGEAPQFPAGA-KVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFV 175 Query: 120 YIGIAGLAFALMYWFVVKPVR------------AARGDAAAEAEKKAINKQAFRTLLKMP 167 +G+ G+A ++ ++ + A D A K ++ +++L Sbjct: 176 IMGVLGIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAK-LSWSEWKSLFTYR 234 Query: 168 LMWQIVAVWFGLSIVNKGLDSWMPTYLMTQRGLNLKSVGLLLPLPYVLAGIATGIGGWV- 226 W ++ + G+ + +W+P YL +R L++ G ++ +PY+ + G++ Sbjct: 235 TTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIA 294 Query: 227 ---MMRFFDG-RERYLLIGSSIL--TAIFVYCMYTSNSVAALITYQCIAYFFKSFVLATC 280 M R R R I + ++ A + YT ++ A I Y +A F + Sbjct: 295 DGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMA-IVYISLAMAFVNMASGGA 353 Query: 281 IALPTKMLPANLIGTSIGMVNLGGQSAGFISPLVIGFLVSAFSNYDYAFSFLIAAACFSV 340 AL + +P L+ + M N GG G ++P++ G +V ++ A A A + Sbjct: 354 WALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAA 413 Query: 341 LVSLFI 346 LV +F+ Sbjct: 414 LVYMFV 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,014,909 Number of extensions: 39757 Number of successful extensions: 312 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 280 Number of HSP's successfully gapped: 29 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)