BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0011.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 178 6e-46 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 155 5e-39 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 111 1e-25 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 96 7e-21 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 69 6e-13 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 178 bits (452), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 135/421 (32%), Positives = 210/421 (49%), Gaps = 22/421 (5%) Query: 1 MIFFRALQGFLGGSMIPMVFTSAFFYFSGHQRVIAAATVGALSSLAPTLGPTVGGWITSN 60 MI R QGF GG++IP T Q+ + A GA + L P LGP +GGW+T N Sbjct: 108 MIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMFGATAILGPVLGPVMGGWLTEN 167 Query: 61 YSWHWLFFINLVPGIFVAVVVPMLVRIDKPNLSLLKGADYLGMILLAASLGCLEYSLEEG 120 SWH+ FFINL G + ++ + + ++P L AD+ G+ LA LG L LEEG Sbjct: 168 VSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEG 227 Query: 121 PRLGWLGDDVIRTTVWISIVAGIGFIWRSLAY---DNPVVDLRALKDRNFALGCFFSFVT 177 R W IR W++ + +GFI ++ +PV+ LR L+DR F + + Sbjct: 228 EREQWFSSPDIR---WLAATSVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIV 284 Query: 178 GIGIFATVYLTPVFLAHVRGFSALDIGLAIFATGLFQICAIPFYTYLGRRVDLRWLMMFG 237 G+ ++ T Y+ P FL+ + G+++L G + +G+ I +PF L R VD+R + G Sbjct: 285 GVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTG 344 Query: 238 MACFALSMW---NFTPITHDWGWRELLLPQALRGFAQQFAVAPVVTLTLGALKPERLKQA 294 A S + + +P+++ + Q +RG A+ + + ++ P + A Sbjct: 345 FLLLAASAFIETDLSPLSYG---GSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDA 401 Query: 295 SGLFNLMRNLGGAIGIAVCGTILNDRTNLHFLRLAEHLNSANESMNNLLQNTGANMLAWG 354 SGL++ RNLGG++ +A I + R LH RL E LN AN ++L+Q++ M A G Sbjct: 402 SGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESLN-AN---SDLVQSS---MTAQG 454 Query: 355 -LDGGSAPTGALQQLWKLTMREAQTQTFADAFLAVMVCFLVAIVLIPLMHKVAPPKAPSA 413 L GG ALQ + +A T T+AD F + V L L+ + + AP A Sbjct: 455 HLLGGQ--DVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVLFLRPLPTHAAPVA 512 Query: 414 D 414 Sbjct: 513 S 513 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 155 bits (392), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 105/350 (30%), Positives = 166/350 (47%), Gaps = 7/350 (2%) Query: 5 RALQGFLGGSMIPMVFTSAFFYFSGHQRVIAAATVGALSSLAPTLGPTVGGWITSNYSWH 64 R LQG + G M+P+ + + + A A G + +AP GP +GGWIT +Y+W Sbjct: 119 RVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWP 178 Query: 65 WLFFINLVPGIFVAVVVPMLVRIDKPNLSLLKGADYLGMILLAASLGCLEYSLEEGPRLG 124 W+F+IN+ G+ A V + R + L D +G+ LL + + L++G L Sbjct: 179 WIFYINIPVGLIAAGVTLAIYRSRETPTHKLP-IDKIGLALLVTWVAAFQIMLDKGKDLD 237 Query: 125 WLGDDVIRTTVWISIVAGIGFIWRSLAYDNPVVDLRALKDRNFALGCFFSFVTGIGI-FA 183 W I T I+++A F+ L D+PVVDLR RNFA G + G G+ F Sbjct: 238 WFASPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFA-GGTIAISIGYGVFFG 296 Query: 184 TVYLTPVFLAHVRGFSALDIGLAIFATGLFQICAIPFYTYLGRRVDLRWLMMFGMACFAL 243 + + P +L G+ ++D GL+ G+F + +P VD R + FA+ Sbjct: 297 NLVILPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAI 356 Query: 244 SMWNFTPITHDWGWRELLLPQALRGFAQQFAVAPVVTLTLGALKPERLKQASGLFNLMRN 303 W + ++LP L+G P+ L L L PER+ A+GL + +R Sbjct: 357 VFWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRV 416 Query: 304 LGGAIGIAVCGTILNDRTNLHFLRLAEHLNSAN----ESMNNLLQNTGAN 349 GA+G ++ T N+R+ +H +L EH + E++NNL G N Sbjct: 417 FCGAVGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLN 466 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 87/326 (26%), Positives = 147/326 (45%), Gaps = 4/326 (1%) Query: 1 MIFFRALQGFLGGSMIPMVFTSAFFYFSGHQRVIAAATVGALSSL-APTLGPTVGGWITS 59 +I FR LQG GG ++P+ T F ++ AA + A+++L AP GP +GG + Sbjct: 124 LIAFRVLQGLAGGPLMPLSQTLLLRIFP-KEKAPAAVGLWAMTTLVAPIAGPILGGVLCD 182 Query: 60 NYSWHWLFFINLVPGIFVAVVVPMLVRIDKPNLSLLKGADYLGMILLAASLGCLEYSLEE 119 YSW ++F+IN+ + + +++ + +S L D +G++L+ + L+ L+E Sbjct: 183 QYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLP-IDRVGLVLMVVWVAALQLMLDE 241 Query: 120 GPRLGWLGDDVIRTTVWISIVAGIGFIWRSLAYDNPVVDLRALKDRNFALGCFFSFVTGI 179 G W I ++V I F+ L NP+VDLR + R F + Sbjct: 242 GKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFG 301 Query: 180 GIFATVYLTPVFLAHVRGFSALDIGLAIFATGLFQICAIPFYTYLGRRVDLRWLMMFGMA 239 F +V LTP++L G++A G TG+ + P + +D R L+ G+ Sbjct: 302 AFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVT 361 Query: 240 CFALSMWNFTPITHDWGWRELLLPQALRGFAQQFAVAPVVTLTLGALKPERLKQASGLFN 299 + T D + ++ P L+G P+ L L ++ E A+GL + Sbjct: 362 WLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMS 421 Query: 300 LMRNLGGAIGIAVCGTI-LNDRTNLH 324 R + GAI ++ T ND T H Sbjct: 422 FCRTMAGAIATSIVNTSWENDATYFH 447 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 95.5 bits (236), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 81/320 (25%), Positives = 147/320 (45%), Gaps = 16/320 (5%) Query: 5 RALQGFLGGSMIPMVFTSAFFYFSGHQRVIAAATVGALSSLAPTLGPTVGGWITSNYSWH 64 R +QG G ++P+ + G Q + A A++ L P +GP +GGW+ + SWH Sbjct: 148 RVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWH 207 Query: 65 WLFFINLVPGIFVAVVVPMLVRIDKPNLSLLKGADYLGMILLAASLGCLEYSLE---EGP 121 W+F INL G+ V L + +L+ D LG +LL+A + +L+ + Sbjct: 208 WIFLINLPVGVLGMGAV--LYFLPDDSLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSH 265 Query: 122 RLGWLGDDVIRTTVWISIVAGIGFI--WRSLA-YDNPVVDLRALKDRNFALGCFFSFVTG 178 R GW ++ + +VA + + WR + + P+ L + NF++G + V Sbjct: 266 RTGW--------SIALFVVAALTVLGYWRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCR 317 Query: 179 IGIFATVYLTPVFLAHVRGFSALDIGLAIFATGLFQICAIPFYTYLGRRVDLRWLMMFGM 238 IG A +L P+ L G+S L G+ + + A P+ L +R ++ Sbjct: 318 IGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNT 377 Query: 239 ACFALSMWNFTPITHDWGWRELLLPQALRGFAQQFAVAPVVTLTLGALKPERLKQASGLF 298 A ++ +F IT DW ++ A+ G + A + ++TL L + LF Sbjct: 378 AIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLF 437 Query: 299 NLMRNLGGAIGIAVCGTILN 318 ++++ L +G+ + GT++N Sbjct: 438 SMVQMLAIGLGVTIGGTMVN 457 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 69.3 bits (168), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 59/191 (30%), Positives = 101/191 (52%), Gaps = 13/191 (6%) Query: 5 RALQGFLGGSMIPMVFTSAFFYFSGHQRVIAA-ATVGALSSLAPTLGPTVGGWITSNYSW 63 R LQG LGG+M+ V +G R++ A AT+ + +AP +GP++GG+IT+ SW Sbjct: 126 RVLQG-LGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASW 184 Query: 64 HWLFFINLVPGIFVAVVVPMLVRIDKPNLSLLKGADYLGMILLAASLGCLEYSLEEGPRL 123 HW+F +N+ G+ V M+VR ++ +S + D +G +L A+L L Y E + Sbjct: 185 HWIFLMNVPFGLAAFTAVMMVVR-NEHGVS-KRPLDLVGFLLSGAALTSLLYGTEIASHM 242 Query: 124 GWLGDDVIRTT---VWISIVAGIGFIWRSLAY-DNPVVDLRALKDRNFALGCFFSFVTGI 179 + I+ V + +V G G WR + ++P++D L+ +++ +T I Sbjct: 243 ----ESNIKVALGFVALGVVLGYG-AWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRI 297 Query: 180 GIFATVYLTPV 190 G+ A YL P+ Sbjct: 298 GVDAVPYLMPL 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,376,934 Number of extensions: 59769 Number of successful extensions: 297 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 11 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)