BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0009.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 165 5e-42 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 151 7e-38 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 140 2e-34 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 75 6e-15 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 165 bits (417), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 120/288 (41%), Positives = 170/288 (59%), Gaps = 1/288 (0%) Query: 1 VPGNIVKVLVTDNQRVKAGDLLIKIDDRDYVAVLEKLDAAVQAQQAALANIDAERRLQEA 60 V G + +VLV DNQ VKAG L++ID RDY A + A + A A+ +A+ Q+A Sbjct: 85 VSGYVDQVLVADNQIVKAGQPLVRIDARDYSAQAAQAKAQIDAAAASEEAANAQLAEQQA 144 Query: 61 VIEQGKAGVAASNADLTRTRDDQTRYQSLSAKSAVSVQSFQKADADYKQAQANGQKAQAG 120 I Q KA +AA++ D + RY L+A A + + QA++ +A Sbjct: 145 AIAQAKAQLAAADEDARFATAEVERYTPLAASGAETRERLTTLHNQQAQARSTVAARRAA 204 Query: 121 LIAAQRQLDVLATQKQKVQAAMQQAVAERNLAQLNVAYTELRAPVDGVVGNRRARTGAYA 180 L AAQ ++ L Q ++ QA + A A+ + A +N+ YTE+RA VDG +G+++ R G + Sbjct: 205 LDAAQLRVRSLQAQVRQAQAQGETAKAQYDAASVNLGYTEIRASVDGRIGDKQVRVGQFV 264 Query: 181 ATGAQLLSVVPADGLWVDANFKESQIARFQTGMTVKIKADVLPGETFEGHVASIAPATGA 240 + G ++++VVP +V ANFKE+QI + G IK D LPG F GHV S++P TGA Sbjct: 265 SPGTRMMTVVPTR-FYVSANFKETQIGIMRIGQPAIIKVDALPGVEFHGHVESLSPGTGA 323 Query: 241 QFSILPAENATGNFTKIVQRVPVRILLDGEAARLGKLRPGLSVTAEVD 288 QFS+LP +NATGNFTKIVQRVPVR+ +D GLSVT EV+ Sbjct: 324 QFSLLPPQNATGNFTKIVQRVPVRVAIDATPEETRAFVAGLSVTVEVN 371 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 151 bits (381), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 111/311 (35%), Positives = 168/311 (54%), Gaps = 23/311 (7%) Query: 1 VPGNIVKVLVTDNQRVKAGDLLIKIDDRDY-------VAVLEKLDAAVQAQQAALANIDA 53 + G + V V D Q VK GD+L++IDD D A L++ + V+ A A + A Sbjct: 80 INGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAA 139 Query: 54 ERRLQEAVIEQGKAGVAASNADLTRTRDDQTRYQSLSAKSAVSVQSFQKADADYKQAQAN 113 + +EA ++ A + ++ ADL R D R ++L+ +VS + A AQAN Sbjct: 140 QVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQAN 199 Query: 114 GQKAQAGLIAAQRQLDVLATQ-KQKVQAAMQQ------------AVAERNLAQLNVAYTE 160 + A+A + Q + ++ ATQ QK + A A+ A+L++ T Sbjct: 200 LKAAEAAEV--QSRANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQAKLDLERTV 257 Query: 161 LRAPVDGVVGNRRARTGAYAATGAQLLSVVPADGLWVDANFKESQIARFQTGMTVKIKAD 220 LRAPVDGV+ R+ + G +GA LLSVVP + VDANFKE Q+ + + G V +KAD Sbjct: 258 LRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKAD 317 Query: 221 VLPGET-FEGHVASIAPATGAQFSILPAENATGNFTKIVQRVPVRILLDGEAARLGKLRP 279 + G + G V ++ TG+ F+++PA+NATGN+ K+VQR+PVRI LD + L Sbjct: 318 LYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRISLDPKELAQRPLSV 377 Query: 280 GLSVTAEVDER 290 GLS+ E+D R Sbjct: 378 GLSMVVEIDTR 388 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 111/294 (37%), Positives = 149/294 (50%), Gaps = 21/294 (7%) Query: 1 VPGNIVKVLVTDNQRVKAGDLLIKIDDRD-----YVAVLEKLDAAVQAQQAALANIDAER 55 V G +V V D Q VKAG L+ +D D A A Q +Q L N Sbjct: 68 VGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFLNN----- 122 Query: 56 RLQEAVIEQGKAGVAASNADLTRTRDDQTRYQSLSAKSAVSVQSFQKADADYKQAQANGQ 115 + A VAA++++L R R+D R Q+ + AVS + A K A A Sbjct: 123 -------DTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALD 175 Query: 116 KAQAGLIAAQRQLDVLATQKQKVQAAMQQAVAERNLAQLNVAYTELRAPVDGVVGNRRAR 175 +A+A A + D + + +Q A A RN A LN A + APV G V R + Sbjct: 176 QARAAAAANRALTDHTSVTEHP--NVLQAATAVRN-AYLNYARVNIVAPVSGFVSKRSVQ 232 Query: 176 TGAYAATGAQLLSVVPADGLWVDANFKESQIARFQTGMTVKIKADVLPGET-FEGHVASI 234 G A G L+++VP + +W+DANFKESQ+ + G V++ ADV ++G V Sbjct: 233 VGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGF 292 Query: 235 APATGAQFSILPAENATGNFTKIVQRVPVRILLDGEAARLGKLRPGLSVTAEVD 288 + TG FS+LPA+NATGN+ K+VQRVPVRI LD E R LR GLS TA VD Sbjct: 293 SAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVD 346 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 75.5 bits (184), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 90/273 (32%), Positives = 136/273 (49%), Gaps = 36/273 (13%) Query: 1 VPGNIVKVLVTDNQRVKAGDLLIKIDDRDYVAVLEKLDAAVQAQQAALANIDAERRLQEA 60 V G +V + V DNQ VK GDLL++ID Y L+ QA+ A A ++A Sbjct: 52 VSGQVVALPVRDNQLVKKGDLLMEIDPARYTLALQ------QAEAAVTA--------RKA 97 Query: 61 VIEQGKAGVAASNADLTRTRDDQTRYQSLSAKSAVSVQSFQKADADYKQAQANGQKAQAG 120 ++ +A V A ADL R R + A VS ++ + A + +QA + A Sbjct: 98 ELDVRRAEVEARRADLNMRRAQARRRADVDAL-VVSREAREDAGSQVTASQAQLEAA--- 153 Query: 121 LIAAQRQLDVLATQKQKVQAAMQQAVAERNLAQLNVAYTELRAPVDGVVGNRRARTGAYA 180 Q Q QA Q A+++R+ A+LN+ T++ +PVDG + N R G YA Sbjct: 154 -----------QAQLQGAQAQYQAALSQRDTARLNLERTKVFSPVDGYITNLNVRRGDYA 202 Query: 181 ATGAQLLSVVPADGLWVDANFKESQIARFQTGMTVKIKADVLPGETFEGHVASIAPAT-- 238 A GA ++V+ + WV A F+E+++ G +I+ ++ G + EGHV SIA Sbjct: 203 AAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRARIQ--LINGASMEGHVDSIARGIYD 260 Query: 239 --GAQFSILPAE-NATGNFTKIVQRVPVRILLD 268 Q L A+ N T N+ ++ QR+PVRI LD Sbjct: 261 RDNPQSRELTADVNPTFNWVRLAQRIPVRIQLD 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.334 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 661,430 Number of extensions: 21591 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 7 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)