BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0007.p (303 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbasp... 122 3e-29 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 85 9e-18 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 84 2e-17 gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 78 9e-16 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 77 1e-15 >gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 122 bits (306), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 100/302 (33%), Positives = 145/302 (48%), Gaps = 28/302 (9%) Query: 28 VRISITAEAADAYFRIRSAQARIRIAEDQVQTSDNLLGLVKLRLRDGLSNQREDALAQAQ 87 V++S+ AE A+AY +R Q R+ + + VQ + +L L R+ G ++ + Q Q Sbjct: 187 VQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQLRIQNQ 246 Query: 88 LSQARSGIPPLRTELERQLNRLDVLMGAVPGTYARELDAGADGNIVVPAISVADG-SAGL 146 L +R+ + PL+ + + LN+L L+G PGT EL A + +P VA G L Sbjct: 247 LDASRADLVPLQADRDAYLNQLATLLGQEPGTLDGELAATVP--VPLPPQQVAVGDPVSL 304 Query: 147 LRRRPDVLAAERKLAASNARIGVAMAEYYPKLSLSALLGF-EXRPC-------------- 191 LRRRPDV AAER LAA A+IG A A YP L L ++G P Sbjct: 305 LRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAP 364 Query: 192 ----------RIDAEVARAKGVNQEALARYRQSILRATEDVENAIVTLVQLEKQSIELAD 241 R A V +A+ V EA +YRQS+L A D E+A+ +A Sbjct: 365 MLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVATIAR 424 Query: 242 EVAARQRARSATQDAYQGGVLSLTEVLEEDRLLLHARDQLAQVRADDARAAVSTFRSLGG 301 + RA + + YQ G +L EVL+ R + A+ L Q A+ R+ V ++LG Sbjct: 425 TKLSADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGL 484 Query: 302 GW 303 GW Sbjct: 485 GW 486 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 84.7 bits (208), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 86/302 (28%), Positives = 149/302 (49%), Gaps = 43/302 (14%) Query: 34 AEAADAYFRIRSAQARIRIAEDQVQTSDNLLGLVKLRLRDGLSNQREDALAQAQLSQAR- 92 A+ A Y ++R A+ ++ E+ VQ + L + + + G+ + + AQ QL A+ Sbjct: 188 AQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQA 247 Query: 93 ----SGIPPLRTELERQLNRLDVLMGAVPGTYARELDAGADGNIVVPAISVADGSAGLLR 148 +G+ R ++E + + +L+G P +A A V P + S LL Sbjct: 248 SALDNGV--TRAQVE---HAIALLVGKTPAQFALP---RAPLAAVAPPVPQGVPSM-LLE 298 Query: 149 RRPDVLAAERKLAASNARIGVAMAEYYPKLSLSALLGFEXR--------PCRID------ 194 RRPDV AAER++A++NA+IGVA A Y+P L+LSA G++ P R+ Sbjct: 299 RRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQL 358 Query: 195 AEV-----ARAKGVNQE------ALARYRQSILRATEDVENAIVTLVQLEKQSIELADEV 243 A+ ARA +Q +A Y+Q++L A ++VE+ + L LE+++ + + Sbjct: 359 AQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEAL 418 Query: 244 AARQRARSATQDAYQGGVLSLTEVLEEDRLLLHARDQLAQVRADDARAAVSTF--RSLGG 301 A ++A + + Y+ G +S V+ L A +L+ + + R A S ++LGG Sbjct: 419 QAARQAVTLVNNQYKAGTVSYVNVITAQATALSA--ELSSLNILNRRMAASVLLAKALGG 476 Query: 302 GW 303 GW Sbjct: 477 GW 478 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 99/336 (29%), Positives = 161/336 (47%), Gaps = 41/336 (12%) Query: 1 VSVXGSFKRGAEAADAERDAAEADHLGVRISITAEAADAYFRIRSAQARIRIAEDQVQTS 60 V + G +AA A+ +EA V +++ A+ A YF +R A + V Sbjct: 166 VDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLR 225 Query: 61 DNLLGLVKLRLRDGLSNQREDALAQAQLSQARS---GIPPLRTELERQLNRLDVLMGAVP 117 L LV+ R +G ++ + A A+A+L+ A+S GI R E + L +L+G P Sbjct: 226 QESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQRATAE---HALAILLGRTP 282 Query: 118 GTYARELDAGADGNIVVPAISVADG-SAGLLRRRPDVLAAERKLAASNARIGVAMAEYYP 176 ++ + A++V G + LL RRPD+ AAER +AA+NAR+GVA + ++P Sbjct: 283 AEFSLPPQP-----LTRLAVTVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFP 337 Query: 177 KLSLSALLGFEX-------------------------RPC----RIDAEVARAKGVNQEA 207 L+L+ LG+E P R A V RA+ +E Sbjct: 338 SLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQ 397 Query: 208 LARYRQSILRATEDVENAIVTLVQLEKQSIELADEVAARQRARSATQDAYQGGVLSLTEV 267 +A YR ++L A +VE+ + L L++Q+ + VAA RA + Y+ G +S V Sbjct: 398 VASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSV 457 Query: 268 LEEDRLLLHARDQLAQVRADDARAAVSTFRSLGGGW 303 ++ DR +L + + + AR+ V+ R+LGGGW Sbjct: 458 IDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 78.2 bits (191), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 96/330 (29%), Positives = 157/330 (47%), Gaps = 32/330 (9%) Query: 1 VSVXGSFKRGAEAADAERDAAEADHLGVRISITAEAADAYFRIRSAQARIRIAEDQVQTS 60 + G + G EAA+A A D V +++T+ Y ++ S + R+ IA VQ + Sbjct: 153 IDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLRDRLAIARQNVQNA 212 Query: 61 DNLLGLVKLRLRDGLSNQREDALAQAQLSQARSGIPPLRTELERQLNRLDVLMGAVPGTY 120 + +L LV+ + R G ++ + A ++ L+ R IP L+ + + L +L+G P + Sbjct: 213 ERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRPPQNF 272 Query: 121 ARELDAGADGNIVVPAISVADGSAGLLRRRPDVLAAERKLAASNARIGVAMAEYYPKLSL 180 E A I +P IS S LL RRPD+ AE LAA+NA + VA A +P ++L Sbjct: 273 KVEEKGLA--RIQMPRISAGLPSE-LLTRRPDIRRAEASLAAANANVAVARAALFPSITL 329 Query: 181 --------SALLG-------------------FEXRPCRIDAEVARAKGVNQEALARYRQ 213 SALL F+ R ++A A+ +E + YRQ Sbjct: 330 TGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQ--KEELVQVYRQ 387 Query: 214 SILRATEDVENAIVTLVQLEKQSIELADEVAARQRARSATQDAYQGGVLSLTEVLEEDRL 273 ++ + +VE A+ + LE++ EV + A + Y+ G L VL+ R Sbjct: 388 RVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRT 447 Query: 274 LLHARDQLAQVRADDARAAVSTFRSLGGGW 303 L A++QL Q++ +A VS +++LGGGW Sbjct: 448 LSEAQNQLGQIKLQRLQATVSLYKALGGGW 477 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 94/326 (28%), Positives = 151/326 (46%), Gaps = 53/326 (16%) Query: 18 RDAAEADHLGV-------RISITAEAADAYFRIRSAQARIRIAEDQVQTSDNLLGLVKLR 70 +DAA A +L +IS+ A+ A AY RS ++++A++ +++ + L L + R Sbjct: 158 KDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKR 217 Query: 71 LRDGLSNQREDALAQAQLSQARSGIPPLRTELERQL----NRLDVLMGAVPGTYARELDA 126 L G S + L Q + AR +T+LERQ N L +L+G G EL Sbjct: 218 LDVGASTALDFRLTQTLVESARVA----KTQLERQRAQAENALTLLVGKKIG----ELPP 269 Query: 127 G---ADGNIV--VPAISVADGSAGLLRRRPDVLAAERKLAASNARIGVAMAEYYPKLSLS 181 +D IV +PA +D LL RPD+ +AE+ L A+NA IG A A ++P++SL+ Sbjct: 270 AQLLSDEKIVTDIPAGLPSD----LLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLT 325 Query: 182 ALLG---------FE------------XRPC----RIDAEVARAKGVNQEALARYRQSIL 216 G FE P R A + A+ +A+ Y ++I Sbjct: 326 GNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQ 385 Query: 217 RATEDVENAIVTLVQLEKQSIELADEVAARQRARSATQDAYQGGVLSLTEVLEEDRLLLH 276 A +V +A+V L++Q +AA+Q T ++ G+ S + L+ R L Sbjct: 386 TAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFS 445 Query: 277 ARDQLAQVRADDARAAVSTFRSLGGG 302 A+ L Q R A+ +++LGGG Sbjct: 446 AQQALIQARQLRLNNAIDLYKALGGG 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 688,212 Number of extensions: 24991 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 17 Length of query: 303 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 218 Effective length of database: 1,217,395 Effective search space: 265392110 Effective search space used: 265392110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)