BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0051.0004.p (181 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312672|ref|YP_003776764.1| TetR family transcription regul... 86 1e-18 >gi|300312672|ref|YP_003776764.1| TetR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 185 Score = 86.3 bits (212), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 45/132 (34%), Positives = 72/132 (54%), Gaps = 1/132 (0%) Query: 3 APTLSRDEVVQRILAE-FRRSGYQGATLASLSEATGLGKSSLYHYFPNGKADMGEAALQA 61 A ++ E V +LAE FR G++GA+LA +SE T LG+ S+YH+FP GK +M +A L Sbjct: 2 ARVIAEKEDVIPLLAEVFRTYGFEGASLARISEGTKLGRGSIYHFFPGGKEEMAQAVLGH 61 Query: 62 VGIWLGENVLPALSEPGSPEKRLKKFSAGLSEFYAKGSKPCLTDLFTIGEAGELYQQYLK 121 V W +V +L+E E +K +S ++ G + CL +F +G + + ++ Sbjct: 62 VDAWFRRHVFESLAEHEDAEAGIKAMFDDVSRYFLSGQRVCLVGVFALGNERDRFAVMVR 121 Query: 122 QRLGGLIKALAA 133 I ALA+ Sbjct: 122 DYFTAWIDALAS 133 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 592,322 Number of extensions: 23198 Number of successful extensions: 67 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 1 Length of query: 181 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 102 Effective length of database: 1,245,805 Effective search space: 127072110 Effective search space used: 127072110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)