BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0045.p (84 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 89 5e-20 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 88 1e-19 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 87 2e-19 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 89.4 bits (220), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 41/56 (73%), Positives = 46/56 (82%) Query: 18 LQFPIYLDYSATTPVDPLVATRMVAYLTEKFGNPASSSHSFGWEAKAAVEKARKQV 73 L+FPIYLDY ATTPVDP VA M +LT+KFGN ASSSH FGWEA+ AVE+AR QV Sbjct: 13 LRFPIYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRAVEQARAQV 68 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 88.2 bits (217), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 41/56 (73%), Positives = 46/56 (82%) Query: 18 LQFPIYLDYSATTPVDPLVATRMVAYLTEKFGNPASSSHSFGWEAKAAVEKARKQV 73 L PIYLDYSATTPVDP VA +M+ YLT+ GNPAS SHSFGW A+AAVE AR+QV Sbjct: 5 LSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQV 60 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 87.4 bits (215), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 37/55 (67%), Positives = 46/55 (83%) Query: 19 QFPIYLDYSATTPVDPLVATRMVAYLTEKFGNPASSSHSFGWEAKAAVEKARKQV 73 FPIY+DYSATTPVDP VA +M+ YL E+FGNPAS SH +GW A+ AVE+AR++V Sbjct: 17 HFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTAEKAVEEAREEV 71 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 248,642 Number of extensions: 7807 Number of successful extensions: 24 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 24 Number of HSP's successfully gapped: 3 Length of query: 84 Length of database: 1,619,870 Length adjustment: 54 Effective length of query: 30 Effective length of database: 1,364,180 Effective search space: 40925400 Effective search space used: 40925400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 123 (52.0 bits)