BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0044.p (267 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 251 4e-68 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 205 3e-54 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 197 1e-51 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 102 3e-23 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 251 bits (641), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 126/163 (77%), Positives = 136/163 (83%) Query: 1 VQAVGKIAIDLAVLKVDLMSFSAHKIYGPKGIGALFVRHNPALRLEAQIDGGGHENGMRS 60 VQA GKIAIDL LKVDLMSFSAHK+YGPKGIGAL+VRHNP L+LEAQIDGGGHENGMRS Sbjct: 194 VQAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEAQIDGGGHENGMRS 253 Query: 61 GTLPTHQIVGMGEAFALAASLQDTEAARIGALRDRLWEGLKDLPGVVLNGSATARIPHNL 120 GTL THQIVGMGEAFALA L + E+ RIGALRDRLW GL DLPGV LNG A R+PHNL Sbjct: 254 GTLATHQIVGMGEAFALAGRLLEEESRRIGALRDRLWAGLADLPGVQLNGCARQRVPHNL 313 Query: 121 NVSFDLDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALGR 163 NVSF ++ QLL IAVSAGSACNSA AAPS+VL A+GR Sbjct: 314 NVSFPPTRHGTLGSQLLGIAVSAGSACNSAAAAPSFVLLAIGR 356 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 205 bits (522), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 105/163 (64%), Positives = 125/163 (76%), Gaps = 2/163 (1%) Query: 2 QAVGKIAIDLAVLKVDLMSFSAHKIYGPKGIGALFVRHNPALRLEAQIDGGGHENGMRSG 61 QA GK+ IDL KVDLMSFSAHK YGPKGIGAL+VR P +R+EAQ+ GGGHE G+RSG Sbjct: 196 QATGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGHERGLRSG 255 Query: 62 TLPTHQIVGMGEAFALAASLQDTEAARIGALRDRLWEGLKDLPGVVLNGSATARIPHNLN 121 TL THQIVGMGEAF LA D+E ARI ALRDRL +GL+++ +NG R+PHNLN Sbjct: 256 TLATHQIVGMGEAFRLAREEMDSELARIRALRDRLAKGLQEIEETYINGDMEHRVPHNLN 315 Query: 122 VSFD-LDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALGR 163 VSF+ ++GE S+ + DIAVS+GSAC SA+ PSYVL ALGR Sbjct: 316 VSFNYVEGE-SLIMAIKDIAVSSGSACTSASLEPSYVLRALGR 357 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 197 bits (500), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 104/162 (64%), Positives = 120/162 (74%), Gaps = 2/162 (1%) Query: 2 QAVGKIAIDLAVLKVDLMSFSAHKIYGPKGIGALFVRHNPALRLEAQIDGGGHENGMRSG 61 QA GK+AIDLA LKVDLMSFSAHK YGPKGIGAL+V P +RLEAQ+ GGGHE G+RSG Sbjct: 185 QATGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLRSG 244 Query: 62 TLPTHQIVGMGEAFALAASLQDTEAARIGALRDRLWEGLKDLPGVVLNGSATARIPHNLN 121 TL THQIVGMGEAF LA TE RI LRDRL G D+ +NG R+PHNLN Sbjct: 245 TLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHNLN 304 Query: 122 VSFD-LDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALG 162 VSF+ ++GE S+ + D+AVS+GSAC SA+ PSYVL ALG Sbjct: 305 VSFNYVEGE-SLLMAIKDVAVSSGSACTSASLEPSYVLRALG 345 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 102 bits (254), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 72/168 (42%), Positives = 97/168 (57%), Gaps = 14/168 (8%) Query: 1 VQAVGKIAIDLAVLKVDLMSFSAHKIYGPKGIGALFVRHNPALRLEAQIDGGGHENGMRS 60 VQAVG++ D+A L VDL+S SAHK++GPKGIGAL+VR L + G E G R Sbjct: 178 VQAVGRVPCDVAGLGVDLLSLSAHKLHGPKGIGALYVRKGIKL---PPLLFGHQERGRRG 234 Query: 61 GTLPTHQIVGMG-EAFALAASLQDTEAARIGALRDRLWEGLKDLPGVVLNGSATARIPHN 119 GT IVG+G A A+L +A + ALR R GL LP V +NG+ AR+P+ Sbjct: 235 GTEDVAGIVGLGVAAAQARAALPRMQA--VSALRQRFERGLARLPQVKINGAGAARLPNT 292 Query: 120 LNVSFDLDGEISVAGQL-----LDIAVSAGSACNSANAAPSYVLTALG 162 ++V F G++ L L I S+GSAC ++ PS+VL A+G Sbjct: 293 VSVRF---GQVHAELVLERLDRLGICASSGSACTASGDMPSHVLMAMG 337 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 783,125 Number of extensions: 31361 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 4 Length of query: 267 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 183 Effective length of database: 1,222,130 Effective search space: 223649790 Effective search space used: 223649790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)