BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0043.p (156 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312099|ref|YP_003776191.1| carboxylesterase [Herbaspirillu... 221 2e-59 >gi|300312099|ref|YP_003776191.1| carboxylesterase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 221 bits (563), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 100/139 (71%), Positives = 123/139 (88%) Query: 1 LHGLCGSSLELGTIPKALKKANCTVVEMQIPNYSASLTDPGVTPNWMDWCAMVESKVVKL 60 LHGLCGS LE+G+IPK LKKA CTV+ + I NYSA+LT+P V+PNW +WCA+VES+V +L Sbjct: 34 LHGLCGSQLEMGSIPKQLKKAGCTVIPLNIDNYSAALTNPRVSPNWEEWCAVVESRVARL 93 Query: 61 KEEHKTVSLCGLSMGATLALAVASFSKEILSVVLLSPVLRYDGWSIPWYHRMMNVPYRLG 120 KEE +TVSLCGLSMGATLALAVA+ SKE+LSVVLLSPVL+YDGWS+PWY +++ V Y LG Sbjct: 94 KEEFRTVSLCGLSMGATLALAVAARSKELLSVVLLSPVLQYDGWSVPWYWKLLTVVYDLG 153 Query: 121 YRNWTYKESDPYGIKNYYM 139 +RNW+YKES+PYGIKN+ M Sbjct: 154 FRNWSYKESEPYGIKNFEM 172 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 460,454 Number of extensions: 16191 Number of successful extensions: 42 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 42 Number of HSP's successfully gapped: 1 Length of query: 156 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 78 Effective length of database: 1,250,540 Effective search space: 97542120 Effective search space used: 97542120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)