BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0042.p (505 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 798 0.0 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 209 4e-55 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 196 3e-51 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 119 4e-28 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 104 2e-23 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 798 bits (2061), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/478 (84%), Positives = 442/478 (92%) Query: 28 LPNSLLGRLSVVMIFGVLVTQLAGGLIWAAQLRTKSEAETRVAAQHLAHSASGAIRYFMT 87 +P+SLLGRLSVVMIFGVLVTQ+AGGLIWA QLR+KSEAETR AAQHLAHSA+ AIRYFMT Sbjct: 1 MPDSLLGRLSVVMIFGVLVTQVAGGLIWAVQLRSKSEAETRTAAQHLAHSAASAIRYFMT 60 Query: 88 LPANYRPIMIQQLREMGGTRFFINANSALVPIPSIASNSLADIAAEAAGETLKSDLPFLP 147 LP NYRP+MIQQLREMGGTRFFINANS+ V + SI + LA +A + A +TL+ DLPFLP Sbjct: 61 LPPNYRPLMIQQLREMGGTRFFINANSSAVAVKSIGDSRLAKLAIDVAAQTLREDLPFLP 120 Query: 148 EFHLSFAWPDNLPVSDDGLKIGDLPDSWVQHILLIKPNPAPVLVIQTQIESGKWLYLAAL 207 E L+FAWPD LPV+ DGL + DLPD+WVQHIL+ KPN APVLVIQ Q+ESG+W+YLAAL Sbjct: 121 EARLAFAWPDGLPVASDGLTVNDLPDNWVQHILMTKPNSAPVLVIQMQVESGRWIYLAAL 180 Query: 208 MPNPYFLDSSNPLSLDRVVLQGLSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVP 267 MPNPYFLDS NPL LDR+VLQGLSLAAILLLTILVVRWTTRPLAAL+DAAEAFGKGETVP Sbjct: 181 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP 240 Query: 268 ELPETGSREFVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELLDDDD 327 LPETGSRE+VKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTEL+DDD Sbjct: 241 SLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA 300 Query: 328 MRTEFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVARMIRDASMAGHEVAFEPS 387 MRT+FHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALV RMIRDA +AGHE+AF PS Sbjct: 301 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPS 360 Query: 388 GINVMAKPLALKRAIGNLFDNALHYGEKVEIAIRQSQALEGDMVEIEIRDHGPGVPEDAM 447 GINV+AKPLALKRAIGNLFDNAL+YG+KVEI+IR S EGD+VEIEIRDHGPGVPEDAM Sbjct: 361 GINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM 420 Query: 448 GSLFQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPQGGLIATIIVPAR 505 G+LFQPYVRLEHGR+ NSGGMGLGLGI+RNIVQAHGGELLLRNHP+GGL+ATII+PAR Sbjct: 421 GTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 478 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 209 bits (533), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 147/489 (30%), Positives = 234/489 (47%), Gaps = 33/489 (6%) Query: 26 RVLPNSLLGRLSVVMIFGVLVTQLAGGLIWA-AQLRTKSEAETRVAAQHLAHSASGAIRY 84 R P +L GRL+++++ G+L Q+ G IW + T E TR+AA HLA A+ Sbjct: 12 RPWPQTLSGRLALILVAGMLAAQMLTGTIWYDMRYGTAMEMPTRLAAAHLAD----AVLI 67 Query: 85 FMTLPANYRPIMIQQLREMGGTRFFIN----ANSALVPIPSIASNSLADIAAEAA----- 135 PA R + L +F ++ + A + +P +A S A+ Sbjct: 68 LDDAPATQRQALAHSLTR---AQFVLSLPDVVDRADLAVPGLAPASSPTTHADQGVVDLF 124 Query: 136 GETLKSDLPFLPEFHLSFAWPDNLPVSDDGLKIGDLPDSWVQHILLIKPNPAPVLVIQTQ 195 G L+ P L D + + SW LL P + Sbjct: 125 GTVLQQHAGPRPWKMLQLGLDD---------QHANAESSW--WTLLRDEFPHGRFRVLVT 173 Query: 196 IESGKWLYLAALMPNPYFLDSSNPLSLDRVV-LQGLSLAAILLLTILVVRWTTRPLAALS 254 ++ G WL + + + D + + L + A++L+ + VR +PL ++ Sbjct: 174 LKDGSWLQADIEEGQAGMSTAPHSAAFDYFMRIYALRIFAVVLIAWMAVRLAMKPLQVMA 233 Query: 255 DAAEAFGKGETVPELPETGSREFVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPIT 314 +AAE G L G E A+AF+ M+ RI + +R R ++SHDLR+PIT Sbjct: 234 EAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPIT 293 Query: 315 RLKLRTELLDDDDMRTEFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVARMIRD 374 R+KLRTE+L D+ + +F DLDE+D ++ L V+D D+ E + LD+L+ + D Sbjct: 294 RMKLRTEMLPDERGKEKFRKDLDEMDALISATLNVVQDIDVQERPQLIDLDSLLESLRTD 353 Query: 375 ASMAGHEVAFEPSGINVMAKPLALKRAIGNLFDNALHYGEKVEIAIRQSQALEGDMVEIE 434 + G EV + P +L+R + NL +NA+ YG++ + ++ + D V I Sbjct: 354 QAEVGGEVTLSGHAAPLQGYPRSLRRCLQNLVENAVRYGQRARVQLQD----DADQVCIR 409 Query: 435 IRDHGPGVPEDAMGSLFQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPQG 494 + D GPG+PE + + +P+ RLE R+A +GG GLGL IA +VQAH G + L N P G Sbjct: 410 VDDDGPGIPEALLERVQEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGG 469 Query: 495 GLIATIIVP 503 GL I++P Sbjct: 470 GLRVEILIP 478 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 196 bits (499), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 154/478 (32%), Positives = 232/478 (48%), Gaps = 32/478 (6%) Query: 31 SLLGRLSVVMIFGVLVTQLAGGLIWAAQLRTKSEAETRVAAQHLAHSASGAIRYFMTLPA 90 S+ R+ +++ G++V A + A R + E R QHLA ++ +P Sbjct: 7 SIGNRIFFLLLGGIVVAVFATNWL-AGNERRNTLRELRF--QHLADRVEQIVQAVDNIPP 63 Query: 91 NYRPIMIQQLREMG-GTRFFINANSALVP--IPSIASNSLADIAAEAAGETLKSDLPFLP 147 RPI++Q G R + N A+ P PS +++ L ++ GE K + Sbjct: 64 AQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIAVQRES 123 Query: 148 EFHLSFAWPDNLPVSDDGLKIG-DLPDSWVQHILLIKPNPAPVLVIQTQIESGKWLYLAA 206 E P D I L D + + L +P P L + + G L Sbjct: 124 ECPPLRTDDARAPRRDTCRVIYISLHDQALMRLRLHQPGEPPAL--RARAGPGGPASLFG 181 Query: 207 LMPNPYFLDSSNPLSLDRVVLQGLSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETV 266 L Y L L L I +L LV R TRP+ L+ AA G Sbjct: 182 LQ---YIL---------------LFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDH 223 Query: 267 PELPETGSREFVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELLDDD 326 L ETG E + A AF+ M+ RI+R ++ R + +I+HDL+TP+TR++LR E + D Sbjct: 224 APLAETGPTEIRQAAHAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDP 283 Query: 327 DMRTEFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVARMIRDASMAGHEVAFEP 386 ++ + DDL + MV+ L + D E + +D+L+ + DA+ AG +VA + Sbjct: 284 GLQQKLIDDLGVMHGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQG 343 Query: 387 SG-INVMAKPLALKRAIGNLFDNALHYGEKVEIAIRQSQALEGDMVEIEIRDHGPGVPED 445 S VMA+P AL+R + NL DNA YG+ ++I ALE + I IRD GPG+PE Sbjct: 344 STRAFVMAQPGALRRCLTNLIDNACKYGKSARVSI----ALEQQKIAIRIRDQGPGIPES 399 Query: 446 AMGSLFQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPQGGLIATIIVP 503 + ++F+P+ RLE RS ++GG GLGL IA NI + H G+L LRN +GGL + +P Sbjct: 400 ELEAVFEPFYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 119 bits (299), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 95/283 (33%), Positives = 137/283 (48%), Gaps = 16/283 (5%) Query: 230 LSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVPELPETGSREFVKTARAFSGMRE 289 LSL + ++ L+ +PLA L+ AA A KG LPE+G E + R+F+ M Sbjct: 172 LSLIGAVFISTLI----NQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVA 227 Query: 290 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELLD-DDDMRTEFHDDLDELDMMVKGALQ 348 + R DR + ISHDLRTP+ R++L E+ + D+ R H DL ++D ++ L Sbjct: 228 DLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLD 287 Query: 349 SVKDSDIHENRTEVRLDALVARMIRDASMAGHEVAFEPS-GINVMAKPLALKRAIGNLFD 407 K D T D L + ++ V + + V L R NL + Sbjct: 288 YAKPFDTSSLETIDLADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELARVFSNLVE 347 Query: 408 NALHYGEK-----VEIAIRQSQALEGDMVEIEIRDHGPGVPEDAMGSLFQPYVRLEHGRS 462 NA YG+ EI IR +EG V +E+ DHGPGVP + L +P+ RL+ R Sbjct: 348 NARRYGKTEGTDCAEIDIRSR--IEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTAR- 404 Query: 463 ANSGGMGLGLGIARNIVQAHGGELLLRNHPQ--GGLIATIIVP 503 + G GLGL I IV H G+LLL+ H + GL+ I +P Sbjct: 405 GQANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 447 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 104 bits (260), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 89/298 (29%), Positives = 141/298 (47%), Gaps = 32/298 (10%) Query: 230 LSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVPEL-PETGSR--EFVKTARAFSG 286 ++L A L+ +L+ + + P+ L +A G EL P +R E R F Sbjct: 170 VALLASLVFALLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADLGRDFDV 229 Query: 287 MRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELLDDDDMRTEFHDDLD--------- 337 M +++ +E + RL +SH+LR+PI RL++ L R + D LD Sbjct: 230 MTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGL-----ARVQDKDKLDLTLERIERE 284 Query: 338 --ELDMMVKGALQ-SVKDSDIHENRTE-VRLDALVARMIRDASMAGHEVAFE-----PSG 388 +D +V L S ++++ + E + ++A++ MI DAS + Sbjct: 285 SVRMDKLVGELLTLSRLEANVPGSMEEPIAIEAMLGDMINDASFEAEAIGKHVDDQLDCR 344 Query: 389 INVMAKPLALKRAIGNLFDNALHY---GEKVEIAIRQSQALEGDMVEIEIRDHGPGVPED 445 + V P L RAI N+ NA+ + G V+I + + ++ + I D GPGVPE+ Sbjct: 345 VQVKGNPELLYRAIENVLRNAIKHTAPGTTVQIHTQLDEI--NKLLHLSILDRGPGVPEE 402 Query: 446 AMGSLFQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPQGGLIATIIVP 503 M S+F+ + R +S NS G GLGL IAR IV+AH G + N GGL I +P Sbjct: 403 EMNSIFELFFRSSRTKS-NSSGHGLGLSIARRIVEAHQGFIQAANRSNGGLRVDITLP 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,628,427 Number of extensions: 71292 Number of successful extensions: 361 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 328 Number of HSP's successfully gapped: 37 Length of query: 505 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 416 Effective length of database: 1,198,455 Effective search space: 498557280 Effective search space used: 498557280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)