BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0031.p (156 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313837|ref|YP_003777929.1| N-acetylglucosamine-6-phosphate... 134 2e-33 >gi|300313837|ref|YP_003777929.1| N-acetylglucosamine-6-phosphate deacetylase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 134 bits (338), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 82/142 (57%), Positives = 94/142 (66%), Gaps = 16/142 (11%) Query: 16 GWIDGAI-SFGDLICSIDGAQLEFEPADGTPHANDGDYILPGFIDLHVHGGGGXTPGGVR 74 G I A+ S L C D A G P DGDY L + VH GGVR Sbjct: 249 GAIRAALRSIPRLFCVTDSTA-----AAGMP---DGDYALGRQV---VH----KCAGGVR 293 Query: 75 LADGTLAGSTLTMDQALRNLIAMGLDISEASLRTSTYPADYLGVKERGRLKPGCFADILV 134 L DGTLAGS LTMDQALRNLI++G+D++EASLRTSTY ADYLG+ +RGRL GC AD++V Sbjct: 294 LDDGTLAGSILTMDQALRNLISLGMDVAEASLRTSTYAADYLGLADRGRLATGCHADVVV 353 Query: 135 FDRQFKLKEVYVEGEKIDLAHA 156 DRQF LK VYVEGE+IDLA A Sbjct: 354 LDRQFALKAVYVEGEEIDLADA 375 Score = 75.5 bits (184), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 38/67 (56%), Positives = 46/67 (68%), Gaps = 4/67 (5%) Query: 1 MSSITQVRGNVLTPHGWIDGAISFGDLICSIDGAQLEFEPADGTPHANDGDYILPGFIDL 60 M++ T++ GNVLTP GW G++ F I SIDG L EP ++GDYILPGFIDL Sbjct: 1 MNTPTELHGNVLTPQGWTHGSMVFDQRIRSIDGRPLRREPRL----IDEGDYILPGFIDL 56 Query: 61 HVHGGGG 67 HVHGGGG Sbjct: 57 HVHGGGG 63 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 500,474 Number of extensions: 19432 Number of successful extensions: 58 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 2 Length of query: 156 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 78 Effective length of database: 1,250,540 Effective search space: 97542120 Effective search space used: 97542120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)