BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0030.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313838|ref|YP_003777930.1| glucosamine 6-phosphate synthas... 144 8e-36 gi|300313709|ref|YP_003777801.1| L-glutamine:D-fructose-6-phosph... 62 3e-11 >gi|300313838|ref|YP_003777930.1| glucosamine 6-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 144 bits (362), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 73/89 (82%), Positives = 77/89 (86%), Gaps = 1/89 (1%) Query: 102 DALRAADS-DWSKAVEILAPATSIMVVGRGIGFPLALEAALKFKETSAIQAEAFSGAEIK 160 DALRAA + DWS AV+ L PA IMVVGRGIGFPLALEAALK KET AIQAEAFSGAEIK Sbjct: 177 DALRAACACDWSDAVQALTPADRIMVVGRGIGFPLALEAALKCKETCAIQAEAFSGAEIK 236 Query: 161 HGPMALINEGYPLLMFATRGPTQAGMLQL 189 HGP+ALI EGYPLLMFA RGP+QAGML L Sbjct: 237 HGPIALIEEGYPLLMFAIRGPSQAGMLAL 265 Score = 122 bits (305), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 69/88 (78%), Positives = 72/88 (81%) Query: 5 MLKEARSAGSHVAEQLARDQARYAELGRWLRTAPPSGIVTVARGSSDHAASYCAYLTMVR 64 MLKEARSA +VA QL RDQ RYA LG LR APP+ IVTVARGSSDHAA+YCAYL M R Sbjct: 1 MLKEARSAADYVAVQLTRDQDRYAALGARLRAAPPANIVTVARGSSDHAATYCAYLIMAR 60 Query: 65 LGHIVASLPMSLVTLNKAPLIARDALAI 92 LG IVASLPMSLVTLN APL RDALAI Sbjct: 61 LGRIVASLPMSLVTLNHAPLQVRDALAI 88 >gi|300313709|ref|YP_003777801.1| L-glutamine:D-fructose-6-phosphate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 605 Score = 62.4 bits (150), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 27/62 (43%), Positives = 42/62 (67%) Query: 116 EILAPATSIMVVGRGIGFPLALEAALKFKETSAIQAEAFSGAEIKHGPMALINEGYPLLM 175 E A + + +GRG+ +P+ALE ALK KE + I AEA++ E+KHGP+AL+ + P++ Sbjct: 456 ESFARRENALFLGRGLHYPIALEGALKLKEITYIHAEAYAAGELKHGPLALVTDQMPVVT 515 Query: 176 FA 177 A Sbjct: 516 IA 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 630,808 Number of extensions: 22902 Number of successful extensions: 80 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 80 Number of HSP's successfully gapped: 3 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)