BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0029.p (666 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 100 5e-22 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 96 1e-20 gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 96 1e-20 gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspiri... 82 2e-16 gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspiri... 81 3e-16 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 100 bits (248), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 80/255 (31%), Positives = 124/255 (48%), Gaps = 22/255 (8%) Query: 399 VRFDLDFDEWNPGGE--SVPLRLAKPFAGAHETGFHFPIIDGTEAAIAFMDGNPNKPYIA 456 V+ +D NPG E S +R++ P AG + P + G E I+F++G+P++P Sbjct: 452 VKVQFRWDRINPGDEKASCWVRVSYPNAGENWGEVKVPRV-GQELIISFLNGDPDRPVAT 510 Query: 457 HLHHNSRQPDHITTEDRW--LSRNVIRTQSNNKLRMEDWKGQEHIKLSTEHSGKSQLNLG 514 N QP T+ R L N+L M+D Q + L + S +QLNLG Sbjct: 511 GRLFNQEQPPQWHTDGRLSGLKSKEFGGNGFNQLVMDDTTSQNRVHLYSSQS-NAQLNLG 569 Query: 515 HLV----DSKRQPRGQGVELRTDAHAAIRGGKGVFINADAQPGAQGKQLDMQAAIANLEA 570 +LV + +R G G L TD + A+ KG+++++ +PG QG QLD A + ++A Sbjct: 570 YLVSQADNERRNFYGSGFALSTDDYGAVVTQKGLYLSSYGRPGPQGTQLDASEATSLMKA 629 Query: 571 SLEQTYALTQATKSAQAIAADYAEQKAFLDDT-----------LKQLKQAGIVMSAPAGI 619 S + L+ + A A A K F+D T + K+ ++M+APAGI Sbjct: 630 SADLARGLSDSASRAGAEALAQDSLKDFVDATEDKYSGQGQGSANRFKKPLLMMAAPAGI 689 Query: 620 ALASGTDVQV-SARE 633 LA+ QV SA+E Sbjct: 690 GLATDQGTQVHSAQE 704 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 95.9 bits (237), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 82/275 (29%), Positives = 128/275 (46%), Gaps = 38/275 (13%) Query: 399 VRFDLDFDEWNPGGE--SVPLRLAKPFAGAHETGFHFPIIDGTEAAIAFMDGNPNKPYIA 456 ++ +D NPG E S +R++ P AG + P I G E ++F++G+P++P + Sbjct: 441 IKVQFRWDRINPGNEAASCWVRVSYPNAGQYWGAIQVPRI-GQEVIVSFLNGDPDRPVVT 499 Query: 457 HLHHNSRQPDHITTEDRW--LSRNVIRTQSNNKLRMEDWKGQEHIKLSTEHSGKSQLNLG 514 N+ Q T R + N++ M+D Q I L + ++ +QLNLG Sbjct: 500 GRLFNAEQRPQWHTNGRLSGVKSKEFGGTGFNQMVMDDTPEQNRIHLYSTNT-HAQLNLG 558 Query: 515 HLV----DSKRQPRGQGVELRTDAHAAIRGGKGVFINADAQPGAQGKQLDMQAAIANLE- 569 HLV + +R G G L TDA+ AI KG++I+ +PGAQG QLD + A L+ Sbjct: 559 HLVSQTGNERRSFFGSGFALSTDAYGAIVTHKGLYISTYGRPGAQGTQLDAREATGQLKS 618 Query: 570 -ASLEQTYALTQATKSAQAIA-------------ADY-----AEQKAFLDDTLKQLKQAG 610 A+L +T + T A A+ +A ADY A+ F L G Sbjct: 619 GANLSRTLSETAAKAGAEPLAGQEALRDFIDATQADYEDPSQAQANRFQQAILLAASPDG 678 Query: 611 IVMSAPAG--------IALASGTDVQVSAREHLIA 637 I ++ P G I L+SG D ++ + L+A Sbjct: 679 IGLTTPKGVHTHAGGEITLSSGADTSIAVGKSLLA 713 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 95.5 bits (236), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 143/633 (22%), Positives = 246/633 (38%), Gaps = 83/633 (13%) Query: 85 RTFVGCITHFNKRKQTHDFHRYEFTIEPLVARLRLTHTSRVFQKKTAPQIIEAILRRHDF 144 R F G + F + YE + P +A L H +R+F + + AI R + Sbjct: 85 RHFTGYVERFAFLRSDGGIAFYEARLVPWLAFLASRHNNRIFHHLSLEGLSAAIFRAYPM 144 Query: 145 NGNQFQFKTRRTYPEHAFRLQYQMSDWDYIHLLMQQEGLHSYFTPGKFGDIIVFGDDIDH 204 + ++ + R P Q+ SD + +H + G H ++ G + DD Sbjct: 145 HA-RWDCQLRHGDPVRTEMFQFDESDSNLLHRRWEDAGWHYHYEHQADGHQLRLSDD--- 200 Query: 205 YIYQPEL----KVPYRETAG-LEANTETVFSLQTQSQTVPASYRVADYHPADAWERFKEE 259 Y + +VP++ G +E + +S Q Q P+S R++ Y A + E Sbjct: 201 STYAKPIDGTGQVPFQRHGGAIEEDGMAQWSAVRQFQ--PSSTRLSSYDFKAARPQEVEV 258 Query: 260 ANIDRADKTTYGQPYVYGSHH--LNQKDAKWEAQLRHEAAIARQIVYTGESNVLELCPAR 317 +++ + Y Y + N+ D +++LR E A V+ G N L P R Sbjct: 259 PTLNKQGAVLQVEHYEYAGAYGFANRDDGDRQSRLRMEEFEAAAQVFEGTGNCRFLQPGR 318 Query: 318 ILRMDQSLPDAPNGQ--------VIVSVTHSGARDAAYKNSYTAIPSDRRFRLQLDEANW 369 R+ + +I+SV HS A + ++S T R R W Sbjct: 319 TFRLTGHFSGSAASHDHDRDPELLIISVEHS-ATNNYLQDSETPPFYANRVRCVRKRIPW 377 Query: 370 ----------PKIAGTLSARVTSPGKYPHAYITKHGHYTVRFDLDFDEWNPGGESVPLRL 419 + G +A V P + ++ ++G V+F D N S LR+ Sbjct: 378 RPGRSYYSQPTALLGIQTATVVGPAG-ENLHVDEYGRVKVQFHWDQIGRNDERSSAWLRV 436 Query: 420 AKPFAGAHETGFHFPIIDGTEAAIAFMDGNPNKPYIAHLHHNSRQPDHITTEDRWLSRNV 479 A ++G + P I G + ++ G+P++P I N T S++ Sbjct: 437 ASSWSGGEQGLVSVPRI-GQLVIVQWLGGHPDRPIITGSVVNQLNMPPWTLP----SQSA 491 Query: 480 IRTQSNNKL--RMEDWKGQE--HIKLSTEHSG----------KSQLNLGHLVDSKRQ--- 522 + + +L R + G H+ H SQL LGH+ +R Sbjct: 492 LSGMRSRELAPRTGNAPGGRSGHVLFDDTHEAIQTQVRSDAFDSQLALGHITRIERHAGR 551 Query: 523 --PRGQGVELRTDAHAAIRGGKGVFINADAQPGAQGKQLDMQAAIANL---EASLEQTYA 577 RG+G ELRTDAH +R +G+ + ++ +P A D+ +A L ++E Sbjct: 552 QDARGEGFELRTDAHGVVRAAQGLLLTSEPRPKAHRHTTDIGETVARLTQARGTVESLSK 611 Query: 578 LTQATKSAQAIAADYAEQKAF--LDDTLK-----------QLKQAGIVMSAPAGIALASG 624 L Q ++ A Y +A +D +K +L + +V+S+P+GI +SG Sbjct: 612 LAQEHEAQDKNADQYDAAQAIKAQNDAIKGDGAAKGGRFPELTEPHLVLSSPSGIEASSG 671 Query: 625 TDVQVSAREH----------LIAKKFFTKNTAE 647 +++ EH L A K F + AE Sbjct: 672 ASTHLASAEHAALTAGSHISLAAGKSFFASAAE 704 >gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 913 Score = 81.6 bits (200), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 83/276 (30%), Positives = 129/276 (46%), Gaps = 43/276 (15%) Query: 413 ESVPLRLAKPFAGAHETGFHFPIIDGTEAAIAFMDGNPNKPYIAHLHHNSRQ-PDHITT- 470 +S +R+A +AG P + G E + F+ G+P+KP + +N + P + Sbjct: 483 DSAWVRVASHWAGRQWGAISLPRV-GDEVLVDFLGGDPDKPIVVGRVYNGQSLPPAFSRV 541 Query: 471 ----EDRWLS---RNVIRTQSNNKLRMEDWKGQEHIKLSTEHSGKSQLNLGHLV----DS 519 +R+L+ I+ Q N+LR++D Q +L+++H G+S+LNLG L Sbjct: 542 GSLPGNRFLAGIKSKEIQGQRYNQLRLDDTPQQISAQLASQH-GQSELNLGWLTHPRAGG 600 Query: 520 KRQPRGQGVELRTDAHAAIRGGKGVFINADAQPGAQGKQLDMQAAIANLEASLEQTYALT 579 + RG+G ELRTD A+RGG+GV I+A + A G QLD +A + L L+ Sbjct: 601 RSDARGEGAELRTDQALALRGGQGVLISAVMRQEASGTQLD-RAEMLGLLHVLQDVQ--Q 657 Query: 580 QATKSAQAIAADYAEQKAF--LDDTLK--------QLKQAG-----IVMSAPAGIALASG 624 Q AQ AD + + F L D L Q K G + ++APAG+A+ S Sbjct: 658 QLATLAQTHHADDTQLQTFSRLRDQLDAWEAGSNTQAKSDGGGKPMVAVTAPAGVAITSD 717 Query: 625 TDVQVSAREHL----------IAKKFFTKNTAESEC 650 V + A++ + A K AES C Sbjct: 718 EGVALGAQKEIDLISVGNTQFSAGKKLLARVAESIC 753 >gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 912 Score = 80.9 bits (198), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 77/303 (25%), Positives = 132/303 (43%), Gaps = 41/303 (13%) Query: 312 ELCPARILRMDQSLPDAPNGQVIVSVTHSGARDAAYKNSYTAIPSDRRFRLQLDEANWPK 371 E PAR DQ+ D P Y N + + SD + D + PK Sbjct: 388 EWLPARAALGDQTALDLP----------------VYCNGFDCVQSDTAIVPRYDPEDVPK 431 Query: 372 IAGTLSARVTSPGKYPHAYITKHGHYTVRFDLDFDEWNP--------GGESVPLRLAKPF 423 + G +SA V G + + G VRF + +P G+S +R+ +P+ Sbjct: 432 L-GLMSAVVVGDGD-KEVDVDELGRIAVRFLFTRPQEHPRGRGASGTTGDSARIRILQPW 489 Query: 424 AGAHETGFHFPIIDGTEAAIAFMDGNPNKPY-IAHLHHNSRQPDHITTEDRW-------- 474 A A +P + G+E IAF+ +P+KP + L+ + P + + + Sbjct: 490 ADAGFGTAFWPRV-GSEILIAFLQNHPDKPVALGGLYDGTHYPPKFSRQGKLPENSALSG 548 Query: 475 LSRNVIRTQSNNKLRMEDWKGQEHIKLSTEHSGKSQLNLGHL----VDSKRQPRGQGVEL 530 + ++ +N L +D +GQ ++ ++H+ +QLN G L + + PRG+G EL Sbjct: 549 IRSKELKANRHNHLLFDDSRGQISAQMFSDHAA-TQLNQGWLGSPRTEGRSAPRGEGFEL 607 Query: 531 RTDAHAAIRGGKGVFINADAQPGAQGKQLDMQAAIANLEASLEQTYALTQATKSAQAIAA 590 TDA +R G+ I A A+ A G QL + +A ++ ++ + + QA A Sbjct: 608 ATDAAGCLRTAHGLLITAFARLNASGNQLAREETLALMQQCVQLFTGIGEYAAQHQAKAG 667 Query: 591 DYA 593 D+A Sbjct: 668 DHA 670 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,089,734 Number of extensions: 87343 Number of successful extensions: 312 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 298 Number of HSP's successfully gapped: 8 Length of query: 666 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 575 Effective length of database: 1,188,985 Effective search space: 683666375 Effective search space used: 683666375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)