BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0021.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 738 0.0 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 664 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 312 4e-86 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 287 1e-78 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 280 2e-76 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 738 bits (1906), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/423 (86%), Positives = 393/423 (92%), Gaps = 1/423 (0%) Query: 1 MTSHTSAKSSKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTF 60 MT+ T A SKF+TVLRVTSGNFMEMFDFFLFGFYA++ISKAFFPS +EFASLMLTFMTF Sbjct: 1 MTTST-ASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTF 59 Query: 61 GAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPGYATIGLLAPALVLI 120 GAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVP YATIG LAP LVLI Sbjct: 60 GAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLI 119 Query: 121 GRLLQGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIVAAAIGYGLNVWLTK 180 GRLLQGFSAGVELGGVSVYLAEMATPG KGFYVSWQSASQQVAIIVAAAIGY L+ LT Sbjct: 120 GRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTP 179 Query: 181 DQVGDWGWRVPFFIGCMIVPVLFVIRRSLQETDEFLARKRKPNLNEIFRSMLENWKLVIA 240 +V WGWR+PFFIGC+IVPVLFVIRRSLQET+EF+ RK +P+ +IFRSM+ENWKLVIA Sbjct: 180 AEVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIA 239 Query: 241 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIG 300 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTE++LIVT CVG+SNFIWLP+MGALSDR+G Sbjct: 240 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVG 299 Query: 301 RRPLLLVFTALTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPV 360 RRPLL+ FT LTILTAYPA+ WLV DA+F KML+VELWLSFLYASYNGAMVVALTEVMP Sbjct: 300 RRPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPA 359 Query: 361 DVRTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATLVLYRK 420 DVRTAGFSLAYSLATAVFGGFTPAIATGLIE THDKAAPG+WMS AAVCGLIATL+LYR Sbjct: 360 DVRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRN 419 Query: 421 KSL 423 K++ Sbjct: 420 KAV 422 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 664 bits (1713), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/427 (77%), Positives = 374/427 (87%) Query: 2 TSHTSAKSSKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFG 61 T+ T+AK SK +TVLRVTSGNF+EMFDFFL+GFYASYISK FFPS +EF SL+LTF TFG Sbjct: 3 TTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFG 62 Query: 62 AGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPGYATIGLLAPALVLIG 121 AGFLMRPLGAIILG+Y+DRVGRRQGLIVTLA+MA+GT+LIAFVPGYATIG+ AP LVL G Sbjct: 63 AGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTG 122 Query: 122 RLLQGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIVAAAIGYGLNVWLTKD 181 RLLQGFSAGVELGGVSVYLAEMATPG KGFYVSWQSASQQ AI+ +A IGY ++ L+ Sbjct: 123 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPV 182 Query: 182 QVGDWGWRVPFFIGCMIVPVLFVIRRSLQETDEFLARKRKPNLNEIFRSMLENWKLVIAG 241 Q+ DWGWR+PFFIGCMIVP +F+IRRSLQETDEFLARKR P+ EI S+ +NWK+V+AG Sbjct: 183 QISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAG 242 Query: 242 MMLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIGR 301 MM+V MTTVSFYLITVYTPTFGKNVLKLS +LIVTFCVG+SNF WLP+MGALSDR+GR Sbjct: 243 MMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGR 302 Query: 302 RPLLLVFTALTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPVD 361 RP+L+ FT LT+LTAYP M+WLV D +F +L+ ELWLSFLYASYNGA VVALTEVMP Sbjct: 303 RPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAH 362 Query: 362 VRTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATLVLYRKK 421 VRT GFSLAYSLATA+FGGFTP ++T LIE + DKAAPG WMSFAAVCGL+ATLVLYR K Sbjct: 363 VRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGK 422 Query: 422 SLAVPVA 428 S A V+ Sbjct: 423 SAAATVS 429 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 312 bits (799), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 176/422 (41%), Positives = 254/422 (60%), Gaps = 11/422 (2%) Query: 1 MTSHTSAKSSKFA------TVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLM 54 M+S+T+A + ++ + GN +E FD ++G +A I+K FFP+GD+ SL+ Sbjct: 1 MSSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL 60 Query: 55 LTFMTFGAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPGYATIGLLA 114 LTF TFG F MRPLGA++LGAY DR GR+ L++++ LM +GT +IAF+P YA+IGLLA Sbjct: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA 120 Query: 115 PALVLIGRLLQGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIVAAAIGYGL 174 PA + +G+++QGFSAG E G + +L E A P R+GF+ SWQ ASQ +++++AA G L Sbjct: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVL 179 Query: 175 NVWLTKDQVGDWGWRVPFFIGCMIVPVLFVIRRSLQETDEFLARKRKPNLNEIFRSMLEN 234 N LT +Q+ WGWRVPF G +I P IRR L E EF ++ N R + Sbjct: 180 NNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF--KESSEKTNAPLRDTFAH 237 Query: 235 WKL-VIAGMMLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMG 293 K+ ++ G V M TVS YL ++Y PT+ L L S+ G+ FI P++G Sbjct: 238 QKMRLLIGAGSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVG 296 Query: 294 ALSDRIGRRPLLLVFTALTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVA 353 ALSD+IGR P ++ +AL I+ YP +L F ++L+++ + L Y AM Sbjct: 297 ALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPAL 356 Query: 354 LTEVMPVDVRTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIA 413 L ++ PV R G SLAY++A +FGGF I T LI+ T DK + ++ F AV LIA Sbjct: 357 LADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIA 416 Query: 414 TL 415 +L Sbjct: 417 SL 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 287 bits (734), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 162/414 (39%), Positives = 236/414 (57%), Gaps = 9/414 (2%) Query: 3 SHTSAKSSKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFGA 62 S+ + + ATV+ GN +E FDF ++ F+A I+K FFP+G++ SL+L TFG Sbjct: 15 SNQQRRRAIVATVI----GNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGV 70 Query: 63 GFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPGYATIGLLAPALVLIGR 122 GF MRP+G I+LG Y DRVGR+ L VT+ LMA+GT +I P Y +IGL AP ++++ R Sbjct: 71 GFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVAR 130 Query: 123 LLQGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIVAAAIGYGLNVWLTKDQ 182 LLQGFSAG E+G + +L E A + +Y SW AS VA+++ AA+G + L Sbjct: 131 LLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAA 190 Query: 183 VGDWGWRVPFFIGCMIVPVLFVIRRSLQETDEFLARKRKPN--LNEIFRSMLENWKLVIA 240 + WGWR+PF +G +I PV + IR L+ET FL K K + L E+ R+ A Sbjct: 191 LASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETS---A 247 Query: 241 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIG 300 +V + TV Y++ Y PT+ VLKL + I G+ + P++G L+DRIG Sbjct: 248 SFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIG 307 Query: 301 RRPLLLVFTALTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPV 360 RRPLL AL ++ AYP ++ + +L+ +L L A+Y G ++ A +E+ P Sbjct: 308 RRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPA 367 Query: 361 DVRTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIAT 414 V + G S+AY+LA +FGGF T LI T AP ++ AA + T Sbjct: 368 KVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 421 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 280 bits (715), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 155/417 (37%), Positives = 236/417 (56%), Gaps = 3/417 (0%) Query: 10 SKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFGAGFLMRPL 69 +K V+ GN +E +DF ++GF+++ I++ FFP+ +E+ SL++ TFG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 70 GAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPGYATIGLLAPALVLIGRLLQGFSA 129 G ++LG Y DR GR+ + V + LM L +IAF P YATIG+ AP ++++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 130 GVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIVAAAIGYGLNVWLTKDQVGDWGWR 189 G E + +L E A ++G Y SWQ Q +A+ A +G + L+ + + WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 190 VPFFIGCMIVPVLFVIRRSLQETDEFLARKRKPNLNEI--FRSMLENWKLVIAGMMLVSM 247 +PF +G +I PV IRR + ETD+FL KPN I + N + V+ M V Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 248 TTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIGRRPLLLV 307 TV+FY++ V PTF L L + A +V +P GALSDR+GRR +LL Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 308 FTALTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPVDVRTAGF 367 + +L YP W+ + ++LV+++ L + + G M AL+E P VR+ G Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 368 SLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATLVLYRKKSLA 424 ++AY+LA +FGGF P I T L + + AP ++ FAA G+ A + R +S A Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAM-RDQSPA 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,254,628 Number of extensions: 51382 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 326 Number of HSP's successfully gapped: 17 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)