BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0016.p (281 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 197 7e-52 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 192 3e-50 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 189 2e-49 gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspir... 174 6e-45 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 74 2e-14 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 197 bits (501), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 126/288 (43%), Positives = 171/288 (59%), Gaps = 26/288 (9%) Query: 2 GSMGTVHLGRQKDYIDDLAPYNGALDFGSLVNNIHALNLDRTQGSRVNNSVRYNSPVFGG 61 G GTV LGRQ D+ D ++ Y DFG +V N +L+R QG+R NNSV Y + G Sbjct: 118 GGFGTVLLGRQTDFADTISAYTAVADFGGVVAN-SGSSLNRLQGTRTNNSVSYTTANLSG 176 Query: 62 VTLSALYGFGEQAGSTGAGQSFGVGGSYNNGPLSFGAAYFQNKLTANNTFTGSSDSGIAP 121 T + +YGFGEQAG T AGQ++G+GG Y+NGPL G Y+Q+K A +T SD+ + P Sbjct: 177 FTGNLIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSK--AGST---PSDTSLIP 231 Query: 122 GAVA-------GSIASTSLKTFLIGGSYKLGDVRLYGSYSQVKQPLAVAAGTAMTGFTGA 174 A A G +T+LK+ + SY+ G R+Y +YS+VKQ L GT Sbjct: 232 AAGATNANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDL---KGT-------P 281 Query: 175 TGAAFTFGGVNNSKVDLIDFGVNYNVTATTQLLANVVTTRSKFVGATSDGKATQVSLGVD 234 T A T N K D + G Y+++ + +LLA V +R+ F G + GK TQ+SLG D Sbjct: 282 TVATATL--ANAQKADTYEIGTAYSLSPSLKLLAAVDHSRATFNGVNTKGKLTQISLGAD 339 Query: 235 YFLSKRTDLYLFYANIRSSDMFNPGLYVAPGGSN-TQNVLTAGIRHKF 281 YFLSKRTDLY F +N+R+SD+ NPG+ GS+ +Q + GIRHKF Sbjct: 340 YFLSKRTDLYAFVSNMRASDIQNPGVTGGATGSDASQTAIITGIRHKF 387 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 192 bits (488), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 118/293 (40%), Positives = 163/293 (55%), Gaps = 27/293 (9%) Query: 2 GSMGTVHLGRQKDYIDDLAPYNGALDFGSLVNNIHALNLDRTQGSRVNNSVRYNSPVFGG 61 G+ G+V LGRQ D+ D ++ Y DFG NLDR +G+R NNS+ Y + G Sbjct: 112 GNFGSVLLGRQTDFADTISSYTSVQDFGGWTG-AAGHNLDRLEGTRTNNSISYTTANLNG 170 Query: 62 VTLSALYGFGEQAGSTGAGQSFGVGGSYNNGPLSFGAAYFQNKL------------TANN 109 T + +YGFGE AG T AGQ+FGVGG Y+NGPL G Y+Q+K T N Sbjct: 171 FTGNLMYGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTN 230 Query: 110 TFTGSSDSGIAPGAVAGSIASTSLKTFLIGGSYKLGDVRLYGSYSQVKQPLAVAAGTAMT 169 FT +++G ST+ K + SY+ G R+YG++S+VKQ L A Sbjct: 231 QFTNGTNNG-----------STAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAA 279 Query: 170 GF-TGATGAAFTFGGVNNSKVDLIDFGVNYNVTATTQLLANVVTTRSKFVGATSDGKATQ 228 G + AT + T N K D+ + G Y+++ + +LLA V TR++F G + K TQ Sbjct: 280 GVGSLATISNRTLAASN--KADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQ 337 Query: 229 VSLGVDYFLSKRTDLYLFYANIRSSDMFNPGLYVAPGGSNTQNVLTAGIRHKF 281 VSLG DY+LSKRTDLY F A +++D+ NPG+ + G Q + GIRHKF Sbjct: 338 VSLGTDYWLSKRTDLYAFLAYSKATDLNNPGVADSSGTDGNQGAVAVGIRHKF 390 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 189 bits (481), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 118/301 (39%), Positives = 167/301 (55%), Gaps = 26/301 (8%) Query: 2 GSMGTVHLGRQKDYIDDLAPYNGALDFGSLVNNIHALNLDRTQGSRVNNSVRYNSPVFGG 61 G+ GTV +GRQ D ++D+ A FG + +H+ LDRT G R+NNS+RYNSP F G Sbjct: 110 GNFGTVLVGRQNDPLNDIGGIASAGGFG-VPGTVHSKGLDRTDGQRLNNSIRYNSPNFNG 168 Query: 62 VTLSALYGFGEQAGSTGAGQSFGVGGSYNNGPLSFGAAYFQNKLTANNTFTGSSDSGIAP 121 ++ S +YGFGEQ G G+S G+G Y NGPLS G YFQ+K+ + GSSD A Sbjct: 169 LSFSGIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGS--DGSSDVN-AK 225 Query: 122 GAVAGS-----IASTSLKTFLIGGSYKLGDVRLYGSYSQVKQPL--------------AV 162 A G+ T LKT+ + +Y+ G ++YG++S V+ PL A+ Sbjct: 226 AACNGAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAM 285 Query: 163 AAGTAMTGFTGATGAAFTFGGVNNSKVDLIDFGVNYNVTATTQLLANVVTTRSKFVGATS 222 TG+ + AF+ GG N+ D G++Y + + L++ V TR+KFVGA S Sbjct: 286 YDAKTGTGYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFVGAAS 345 Query: 223 DGKATQVSLGVDYFLSKRTDLYLFYANIRSSDMFNPGLYV--APGGSNTQNVLTAGIRHK 280 G+ Q++LG Y LSKRT Y N+RSSDM+N GL PG +Q ++ G+ H Sbjct: 346 -GRLLQLNLGAKYDLSKRTSAYALVRNLRSSDMYNVGLSKDQEPGIDRSQTAISTGLIHT 404 Query: 281 F 281 F Sbjct: 405 F 405 >gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 372 Score = 174 bits (442), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 106/291 (36%), Positives = 161/291 (55%), Gaps = 37/291 (12%) Query: 2 GSMGTVHLGRQKDYIDDLAPYNGALDF--GSLVNNIHALNLDRTQGSRVNNSVRYNSPVF 59 G GT+ +GRQ DY++++ + G V H +LDR G+R NNS+R+++ + Sbjct: 108 GRFGTLTVGRQTDYLEEIGQQYTSFQIFGGGGVRAAHFNSLDRITGARSNNSLRFDTAEY 167 Query: 60 GGVTLSALYGFGEQAGSTGAGQSFGVGGSYNNGPLSFGAAYFQNKLTANNTFTGSSDSGI 119 GG+ S YG GE AGST +GQS GVGG++ G + GAAYFQ+KL ++ Sbjct: 168 GGLRGSFYYGAGEVAGSTRSGQSLGVGGNFRRGNVGVGAAYFQSKLASD----------- 216 Query: 120 APGAVAGSIASTSLKTFLIGGSYKLGDVRLYGSYSQVKQPLAVA-AGTAMTGFTGATGAA 178 A+ T LKTF +G SY+ G +L+ ++SQ+++P A + + T AT Sbjct: 217 ---ALPARAGDTELKTFTLGASYQAGPAKLFAAWSQIRRPTQQPLASSGLVAITSAT--- 270 Query: 179 FTFGGVNNSKVDLIDFGVNYNVTATTQLLANVVTTR---SKFVGATSDGKATQVSLGVDY 235 + +++D GV+Y + + LLA+V+ R S+ G + G TQV+LGVD Sbjct: 271 ---------RANVLDVGVDYALDSKLHLLASVIHDRADLSRAAGGATRGSTTQVNLGVDL 321 Query: 236 FLSKRTDLYLFYANIRSSDMFNPGLY-----VAPGGSNTQNVLTAGIRHKF 281 LSKRTD+Y + ++ D+ NPG+ AP ++QNVL G+RHKF Sbjct: 322 LLSKRTDVYAMASRQQARDVLNPGIISAAYSSAPADDSSQNVLRVGLRHKF 372 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 81/295 (27%), Positives = 119/295 (40%), Gaps = 71/295 (24%) Query: 4 MGTVHLGRQKDYIDD---------LAPYNGALDFGSLVNNIH--ALNLDRTQGSRVNNSV 52 G++ +GRQ D++ + L+P G+ +G + H ALN G R NNS+ Sbjct: 112 WGSLSVGRQYDFMANIGGQYAMGALSP-AGSFGWGLHADAAHGTALNNHIYAGDRTNNSI 170 Query: 53 RYNSPVFGGVTLSALYGFGEQAGSTGAGQSFGVGGSYNNGPLSFGAAYFQNKLTANNTFT 112 +YNS FGG + A+ G GE AG+ AG++ G Y+NGP S AY Sbjct: 171 KYNSAKFGGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTD---------- 220 Query: 113 GSSDSGIAPGAVAGSIASTSLKTFLIGGSYKLGDVRLYGSYSQVKQPLAVAAGTAMTGFT 172 + S S S + + G Y++G +G +QVK Sbjct: 221 -----------IKDSTGSFSTRIYGGGARYQIGAFTPFGVVTQVKNT------------- 256 Query: 173 GATGAAFTFGGVNNSKVDLIDFGVNYNVTATTQLLANV-VTTRSKFVGATSDGKATQVSL 231 ++K + G Y +T L A R++ V G A + Sbjct: 257 -----------NTSAKATTYELGTVYALTPLWDLAAAYQFQKRNQGV-----GNAQALVA 300 Query: 232 GVDYFLSKRTDLYLFYANIRSS-----DMFNPGLYVAPGGSNTQNVLTAGIRHKF 281 VDY +SKRTDLY+ R +F G+ A G Q L G+RH+F Sbjct: 301 TVDYKISKRTDLYVGAVYDRDKGYNAYPVFGGGIQSATG---NQTALRLGMRHRF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 809,971 Number of extensions: 32170 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 128 Number of HSP's successfully gapped: 11 Length of query: 281 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 197 Effective length of database: 1,222,130 Effective search space: 240759610 Effective search space used: 240759610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)