BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0006.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate A... 485 e-138 gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate A... 334 6e-93 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 94 2e-20 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 92 4e-20 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 86 5e-18 >gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 289 Score = 485 bits (1248), Expect = e-138, Method: Compositional matrix adjust. Identities = 250/283 (88%), Positives = 271/283 (95%) Query: 8 FVRPEIVVEPNRNAVAEVQRPLSAFEAIYRISWIRKTAILLVLALVWEIYGRWLDNSLLF 67 FVRPEI+V+PN +A AEVQRPLSAFE++YR+ W+RKT ILLVLA +WE+YGRWLDN+LLF Sbjct: 7 FVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWLDNALLF 66 Query: 68 PSFSETLHAFMSGISSGVLLQRAAVSLQTLLIGYSLGILLAGLLTTVAISSRIGNDFLET 127 PSFSET+ AF+SGI SGVLLQRAAVS+QTLL+GY LGILLAGLLTTVAISSRIGND LET Sbjct: 67 PSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIGNDLLET 126 Query: 128 LTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHAGFRSVSNTLRMVGQ 187 LTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTH GFR+VSNTLRMVGQ Sbjct: 127 LTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNTLRMVGQ 186 Query: 188 NYGLKRFSLVRSVLIPAAFPSILTGLKIGWAFAWRTLIAAELVFGVSSGTGGLGWYIFEN 247 NYGL+RFSLV+S+LIPAAFPSILTGLK+GWAFAWRTLIAAELVFGVSSG GGLGWYIFEN Sbjct: 187 NYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLGWYIFEN 246 Query: 248 RNQLETANVFSGLFFVIIIGLFVENMIFAKIEKRTIRRWGMQH 290 RNQLETANVFSGLFFVIIIGL VEN+IFA+IEKRTIRRWGMQH Sbjct: 247 RNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 >gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 334 bits (856), Expect = 6e-93, Method: Compositional matrix adjust. Identities = 171/290 (58%), Positives = 215/290 (74%), Gaps = 2/290 (0%) Query: 1 MTKNSTLFVRPEIVVEPNRNAVAEVQRPLSAFEAIYRISWIRKTAILLVLALVWEIYGRW 60 M + + EI + P V +QR S + +++ W+RK AILL+LALVWE RW Sbjct: 1 MAQTPPIRDEYEIPLTPLPGLV--LQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARW 58 Query: 61 LDNSLLFPSFSETLHAFMSGISSGVLLQRAAVSLQTLLIGYSLGILLAGLLTTVAISSRI 120 +N LL PSF +T AF+ G+ SG L + +SLQ LL Y LGI+ A LTT+A+S++ Sbjct: 59 QNNDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQF 118 Query: 121 GNDFLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHAGFRSVSN 180 G D L TLTSMFNPLPAIALLP+AL+WFGLG GS+IFVLVHSVLWA+A+NT+AGF VS+ Sbjct: 119 GRDLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSD 178 Query: 181 TLRMVGQNYGLKRFSLVRSVLIPAAFPSILTGLKIGWAFAWRTLIAAELVFGVSSGTGGL 240 TLRM G+NYGL+ V VL+PAA PSIL GL+IGWAFAWRTLIAAELVFG +SG GGL Sbjct: 179 TLRMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGL 238 Query: 241 GWYIFENRNQLETANVFSGLFFVIIIGLFVENMIFAKIEKRTIRRWGMQH 290 GWYIF+NRN+L T VF+GL VI+IGL VEN++F +E+ T+++WGMQ Sbjct: 239 GWYIFQNRNELYTDKVFAGLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 74/235 (31%), Positives = 116/235 (49%), Gaps = 9/235 (3%) Query: 54 WEIYGR--WLDNSLLFPSFSETLHAFMSGISSGVLLQRAAVSLQTLLIGYSLGILLAGLL 111 WE+ + WL + +L P L A +SG L + AVS G+++G L +L Sbjct: 47 WEVAAQAGWLSSRIL-PEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLIL 105 Query: 112 TTVAISSRIGNDFLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNT 171 ++ S R L+T M +PA+AL+PL ++WFG+ + +F++ V + V LNT Sbjct: 106 GLLSGSLRSAELLLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNT 165 Query: 172 HAGFRSVSNTLRMVGQNYGLKRFSLVRSVLIPAAFPSILTGLKIGWAFAWRTLIAAELVF 231 G RSV L + ++YGL + L R V++P A P+IL GL+ W LI AE + Sbjct: 166 FHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETI- 224 Query: 232 GVSSGTGGLGWYIFENRNQLETANVFSGLFFVIIIGLFVENMIFAKIEKRTIRRW 286 S G+G+ R L+T V G+ ++G + A+ +R RW Sbjct: 225 ---SAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADTA--ARWLERFWLRW 274 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 66/243 (27%), Positives = 123/243 (50%), Gaps = 8/243 (3%) Query: 29 LSAFEAIYRISWIRKTAILLVLALVWEIYGRWLDNSLLFPSFSETLHAFMSGISSGVLLQ 88 ++ +E R WI +L W + +W++ + + ++++ SG L+ Sbjct: 20 IALWEGASRAGWINPQ----ILPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFSGELVM 75 Query: 89 RAAVSLQTLLIGYSLGILLAGLLTTVAISSRIGNDFLETLTSMFNPLPAIALLPLALIWF 148 A SL ++ G+ +G LA L + SS+ + + P+P IA +PLA++WF Sbjct: 76 DAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIPLAILWF 135 Query: 149 GLGTGSIIFVLVHSVLWAVALNTHAGFRSVSNTLRMVGQNYGLKRFSLVRSVLIPAAFPS 208 GLG +F++ + V +NT AG R V + +N G + +L V++PAA P Sbjct: 136 GLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVMLPAAVPY 195 Query: 209 ILTGLKIGWAFAWRTLIAAELVFGVSSGTGGLGWYIFENRNQLETANVFSGLFFVIIIGL 268 IL+G++IG A+ +I AE++ + + GLG+ I E R + + +G+ + ++GL Sbjct: 196 ILSGVRIGIGTAFIVVIVAEMI----AVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGL 251 Query: 269 FVE 271 ++ Sbjct: 252 AID 254 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 79/283 (27%), Positives = 124/283 (43%), Gaps = 17/283 (6%) Query: 17 PNRNAVAEVQRPLSAFEAIYR----ISWIRKTAILLVLALVWEIYGRW-LDNSLLFPSFS 71 P + A R + YR WI A LL++ E R L + L P S Sbjct: 2 PTSTSTARTHRRGWPHDDAYRPGRFTGWIVPLAFLLLV----EAGARLELIPARLLPPPS 57 Query: 72 ETLHAFMSGISSGVLLQRAAVSLQTLLIGYSLGILLAGLLTTVAISSRIGNDFLETLTSM 131 E H S G LL A SL + G+ +G LA L + SR LE Sbjct: 58 ELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQA 117 Query: 132 FNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHAGFRSVSNTLRMVGQNYGL 191 +P++A +PL L+W G+ + ++ + V LN AG R + + L VG GL Sbjct: 118 LRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGL 177 Query: 192 KRFSLVRSVLIPAAFPSILTGLKIGWAFAWRTLIAAELVFGVSSGTGGLGWYIFENRNQL 251 +L+ +L+PA+ P + GL+ G + AW L+AAEL+ + T G+G+ + + R Sbjct: 178 NTQALIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELI----AATKGVGYLLSDGRESS 233 Query: 252 ETANVFSGLFFVIIIGLFVENMIFAKIEK----RTIRRWGMQH 290 V + + + ++G + ++ A +K R RR Q Sbjct: 234 RPDLVLAAILILALLGKLSDGLLRAVEQKSLGWREARRQSSQQ 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 823,434 Number of extensions: 31671 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 14 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)