BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0048.0003.p (121 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspir... 71 2e-14 >gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 355 Score = 70.9 bits (172), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 37/58 (63%), Positives = 43/58 (74%), Gaps = 1/58 (1%) Query: 65 LFMAGANYSLSKRTLLYVGAGTVRNSSTANFSVEA-SNNNPLPGQNQNGAYAGISHSF 121 LFMAG NY LSKRTLLYVG GT+RN + AN+S+EA S+ G NQ G Y GI+HSF Sbjct: 298 LFMAGVNYFLSKRTLLYVGGGTMRNGNNANYSLEADSSTGGGKGTNQTGGYFGIAHSF 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 285,087 Number of extensions: 7913 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 121 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 47 Effective length of database: 1,269,480 Effective search space: 59665560 Effective search space used: 59665560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 125 (52.8 bits)